Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out

Abstract

The UV-nanoimprint lithography(UV-NIL) fabrication of a novel network of micron-sized channels, forming an open channel microfluidic system is described. Details about the complete manufacturing process, from mastering to fabrication in small batches and in high throughput with up to 1200 micro titer plates per hour is presented. Deep insight into the evaluation of a suitable UV-curable material, mr-UVCur26SF is given, presenting cytotoxic evaluation, cell compatibility tests and finally a neuronal assay. The results indicate how the given pattern, in combination with the resist, paves the way to faster, cheaper, and more reliable drug screening.

Rotating Cylinder‐Assisted Nanoimprint Lithography for Enhanced Chemisorbable Filtration Complemented by Molecularly Imprinted Polymers

“…In particular, our newly developed technique is applicable not only to rigid and flexible substrates but to perforated and uneven substrates, unlike other previous reports. [ 28,29 ] The main advantageous feature was to carefully control the local pressure of rolling mold in the NIL system. Furthermore, a custom‐made MIP‐resin was successfully applied to the nanopatterning process to provide selective binding sites to capture volatile organic compounds.…”

Editorial for Special Issue: Nanoimprint Lithography Technology and Applications

“…One of the exciting things about nanoimprinting is the big versatility of the process and the broad range of applications it can be used for. This special issue includes 10 articles [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ] which represent a small glimpse of the challenges and possibilities of this technology.…”

“…Six contributions deal with nanoimprint processes aiming at specific applications [ 2 , 4 , 6 , 8 , 9 , 10 ], while the other four papers focus on more general aspects of nanoimprint processes [ 3 , 5 , 7 ] or present novel materials [ 1 , 3 ]. Several different types of nanoimprint processes are used: plate-to-plate [ 1 , 2 , 3 , 5 , 7 , 10 ], roll-to-plate [ 4 , 6 ], and roll-to-roll [ 8 ]. Plate-to-plate NIL here also includes the use of soft and flexible stamps.…”

“…A novel concept of nanoimprint-fabricated micro titer plates for neuronal cells is presented by Lohse et al in [ 8 ]. Roll-to-roll nanoimprinting is used in this paper as a fabrication method.…”

“…In summary the application fields in this special issue can be identified as plasmonics [ 2 ], superhydrophibicity [ 4 ], biomimetics [ 4 , 9 , 10 ], optics/datacom [ 6 ], and life sciences [ 8 ]. The nanoimprint process related papers discuss filling and wetting aspects during nanoimprinting [ 3 , 5 , 7 ] as well as materials for stamps [ 3 ] and imprinting [ 1 ].…”