Novel complexes of Co(III) and Ni(II) containing peptide ligands: Synthesis, DNA binding and photonuclease activity

Sudhamani, C.N.; Naik, H.S. Bhojya; Girija, D.; Gowda, K R Sangeetha; Giridhar, M.; Arvinda, T.

doi:10.1016/j.saa.2013.08.074

Cited by 17 publications

(7 citation statements)

References 38 publications

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“…The concentration of stock solution was estimated via UV absorption at 260 nm, taking the molar absorption coefficient as 6600 M −1 cm −1 . UV absorption at 260 and 280 nm had a ratio ( A 260 / A 280 ) of 1.83 (usually around 1.8–1.9), which indicated that the prepared CT‐DNA solution was sufficiently free of protein contamination . The stock solution was stored at 4°C and was utilized within 4 days.…”

Section: Methodsmentioning

confidence: 99%

“…Spectrophotometric scan experiments were executed by keeping the metal chelates at a constant molar concentration while changing the concentration of DNA. The absorption due to free CT‐DNA was removed through using equimolar CT‐DNA to formulate a buffer solution in the blank compartment and the spectra were considered to result from the metal chelates and the DNA–metal chelate aggregation . From the absorption data, the intrinsic binding constant ( K b ) was determined by way of plotting [DNA]/( ε a − ε f ) versus [DNA] according to the following expression:

\frac{([], DNA)}{ε_{a} - ε_{f}} = \frac{([], DNA)}{ε_{b} - ε_{f}} + \frac{1}{K_{b} ((), ε_{b} - ε_{f})}

where [DNA] is the concentration of DNA in base pairs and ε a , ε f and ε b are the apparent, free and completely bound metal chelate extinction coefficients, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The information was reasonable for the above equation with a slope equivalent to 1/( ε b − ε f ) and y ‐intercept equivalent to 1/ K b ( ε b − ε f ); K b was obtained from the ratio of slope to intercept. The standard Gibbs free energy for DNA binding was assessed from the following expression:

normalΔ G_{b}^{\neq} = - italicRT ln K_{b}

…”

Section: Methodsmentioning

confidence: 99%

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Some new Ag(I), VO(II) and Pd(II) chelates incorporating tridentate imine ligand: Design, synthesis, structure elucidation, density functional theory calculations for DNA interaction, antimicrobial and anticancer activities and molecular docking studies

Abdel‐Rahman

Abu‐Dief

Shehata

et al. 2019

Applied Organom Chemis

111

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Some new complexes derived from VO(II), Ag(I) and Pd(II) metal ions and HNA imine ligand (L), i.e. (2‐((6‐allylidene‐2‐hydroxycyclohexa‐1,3‐dienylmethylene)amino)benzoic acid), have been prepared and their structures elucidated via molar conductance measurements, elemental analyses, infrared, NMR and electronic spectra and magnetic susceptibility estimations. Moreover, stability constants of the synthesized complexes were evaluated utilizing a spectrophotometric technique. On the basis of molar conductance and elemental analyses, the metal imine chelates have structure [M(L)], where M = Pd(II), VO(II) and Ag(I). The results indicate that the prepared HNA imine ligand acts as a tridentate moiety via nitrogen atom of azomethine group and two oxygen atoms of phenolic and carboxylic groups. All the complexes are found to be monomeric with 1:1 stoichiometry with square planar geometry for Pd(II), tetrahedral geometry for Ag(I) and distorted square pyramidal for VO(II). Theoretical density functional theory calculations were applied to verify the molecular geometry of the chelators and their metal chelates. The geometry optimization results are in agreement with experimental observations. The antimicrobial properties of the prepared HNA imine ligand and its metal chelates were evaluated against numerous plant pathogenic fungi and bacteria. The results of these studies indicate that the metal complexes exhibit a stronger antibacterial and antifungal effect compared to the imine ligand. In addition, the interaction of the metal imine chelates with calf thymus DNA was observed by way of viscosity, gel electrophoreses and spectral studies. Absorption titration studies reveal that each of the complexes is an avid binder to calf thymus DNA. Also, there are appreciable changes in the relative viscosity of DNA, which are consistent with enhanced hydrophobic interaction of the aromatic rings and intercalation mode of binding. Additionally, the cytotoxic activity of the investigated compounds against various cancer cell lines shows promising results which makes them prospective compounds for antibiotic and anticancer medicament studies. Furthermore, docking studies of the prepared compounds were conducted for confirming the biological results.

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Section: Methodsmentioning

confidence: 99%

\frac{([], DNA)}{ε_{a} - ε_{f}} = \frac{([], DNA)}{ε_{b} - ε_{f}} + \frac{1}{K_{b} ((), ε_{b} - ε_{f})}

where [DNA] is the concentration of DNA in base pairs and ε a , ε f and ε b are the apparent, free and completely bound metal chelate extinction coefficients, respectively.…”

Section: Methodsmentioning

confidence: 99%

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Some new Ag(I), VO(II) and Pd(II) chelates incorporating tridentate imine ligand: Design, synthesis, structure elucidation, density functional theory calculations for DNA interaction, antimicrobial and anticancer activities and molecular docking studies

Abdel‐Rahman

Abu‐Dief

Shehata

et al. 2019

Applied Organom Chemis

111

View full text Add to dashboard Cite

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“…The titration processes were repeated until there was no change in the spectra, indicating binding saturation had been achieved. The absorption data were estimated for an evaluation of the intrinsic binding constant ( K b ) of the complexes with CT‐DNA …”

Section: Biological Activitymentioning

confidence: 99%

Ni(II) and Cu(II) complexes with ONNO asymmetric tetradentate Schiff base ligand: synthesis, spectroscopic characterization, theoretical calculations, DNA interaction and antimicrobial studies

Abdel‐Rahman

Abu‐Dief

Moustafa

et al. 2016

Applied Organom Chemis

137

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A novel Schiff base, namely Z‐3‐((2‐((E)‐(2‐hydroxynaphthyl)methylene)amino)‐5‐nitrophenylimino)‐1,3‐dihydroindin‐2‐one, was synthesized from the condensation of 2‐hydroxy‐1‐naphthaldehyde and isatin with 4‐nitro‐o‐phenylenediamine. It was structurally characterized on the basis of 1H NMR, 13C NMR and infrared spectra and elemental analyses. In addition, Ni(II) and Cu(II) complexes of the Schiff base ligand were prepared. The nature of bonding and the stereochemistry of the investigated complexes were elucidated using several techniques, including elemental analysis (C, H, N), Fourier transform infrared and electronic spectroscopies and molar conductivity. The thermal behaviours of the complexes were studied and kinetic–thermodynamic parameters were determined using the Coats–Redfern method. Density functional theory calculations at the B3LYP/6‐311G++ (d, p) level of theory were carried out to explain the equilibrium geometry of the ligand. The optimized geometry parameters of the complexes were evaluated using LANL2DZ basis set. The total energy of highest occupied and lowest unoccupied molecular orbitals, Mullikan atomic charges, dipole moment and orientation are discussed. Moreover, the interaction of the metal complexes with calf thymus DNA (CT‐DNA) was explored using electronic spectra, viscosity measurements and gel electrophoresis. The experimental evidence indicated that the two complexes could strongly bind to CT‐DNA via an intercalation mechanism. The intrinsic binding constants of the investigated Ni(II) and Cu(II) complexes with CT‐DNA were 1.02 × 106 and 2.15 × 106 M−1, respectively, which are higher than that of the standard ethidium bromide. Furthermore, the bio‐efficacy of the ligand and its complexes was examined in vitro against the growth of bacteria and fungi to evaluate the antimicrobial potential. Based on the obtained results, the prepared complexes have promise for use as drugs. Copyright © 2016 John Wiley & Sons, Ltd.

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“…The application of viscosity measurements of solution in DNA binding studies is one of the most useful techniques carried out in the absence of crystallographic structural data or NMR spectra. A classical intercalation causes the elongation and/or rigidification of the DNA helix in solution, leading to an increase in the specific viscosity (Sudhamani et al, 2014). In contrast partial intercalation trend to bend or kink of the DNA helix, resulting reduction in its effective length, thereby decrease in the viscosity (Raja et al, 2005).…”

Section: Viscosity Measurementsmentioning

confidence: 99%

Synthesis, spectroscopic studies of novel N-substituted phthalimides and evaluation of their antibacterial, antioxidant, DNA binding and molecular docking studies

Nayab

Pulaganti

Chitta

et al. 2015

Bangladesh J Pharmacol

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A new series of N-substituted phthalimide derivatives were prepared by condensation of appropriate amount of n-amino tetrachlorophthalimide with respective aldehyde in glacial acetic acid. The structural investigation of the synthesized compounds was done by spectroscopic methods (UV-Vis., IR, 1 H and 13 C NMR) and elemental analysis. The antibacterial screening of these compounds was performed against Escherichia coli and Staphylococcus mutans. The synthesized compounds were evaluated for their antioxidant potential using 2,2-diphenyl-1-picrylhydrazyl (DPPH) as a scavenging agent. The interaction ability of most promising compounds (3a and 3b) with native calf thymus DNA (Ct-DNA) was also studied by means of UV-Vis., circular dichroism (CD), viscosity measurements and thermal studies. The intrinsic binding constants (Kb) of 3a and 3b with Ct-DNA obtained from UV-Vis. absorption studies were 8 × 10 4 and 1 × 10 5 , respectively. Molecular docking of target compounds (3a and 3b) against DNA dodecamer d (CGCGAATTCGCG)2 has been carried out. The test compounds exhibited remarkable antibacterial, antioxidant and DNA binding activities. Article Info

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Novel complexes of Co(III) and Ni(II) containing peptide ligands: Synthesis, DNA binding and photonuclease activity

Cited by 17 publications

References 38 publications

Some new Ag(I), VO(II) and Pd(II) chelates incorporating tridentate imine ligand: Design, synthesis, structure elucidation, density functional theory calculations for DNA interaction, antimicrobial and anticancer activities and molecular docking studies

Some new Ag(I), VO(II) and Pd(II) chelates incorporating tridentate imine ligand: Design, synthesis, structure elucidation, density functional theory calculations for DNA interaction, antimicrobial and anticancer activities and molecular docking studies

Ni(II) and Cu(II) complexes with ONNO asymmetric tetradentate Schiff base ligand: synthesis, spectroscopic characterization, theoretical calculations, DNA interaction and antimicrobial studies

Synthesis, spectroscopic studies of novel N-substituted phthalimides and evaluation of their antibacterial, antioxidant, DNA binding and molecular docking studies

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