Novel chitosan-based pH-responsive lipid-polymer hybrid nanovesicles (OLA-LPHVs) for delivery of vancomycin against methicillin-resistant Staphylococcus aureus infections

Hassan, Daniel; Omolo, Calvin A.; Fasiku, Victoria Oluwaseun; Mocktar, Chunderika

doi:10.1016/j.ijbiomac.2020.01.019

Cited by 50 publications

(17 citation statements)

References 120 publications

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“…The electrical conductivity and DNA and protein leakage studies were performed as per previously reported methods [ 50 ] and details can be found in the supplementary material, Section S7 .…”

Section: Methodsmentioning

confidence: 99%

“…For the negative control, sterile deionized water was added into the bacterial MHB alone, and bacterial cell viability was observed for up to 24 h. Tested materials, bacteria alone, bare VCM and VCM-StBAclm-Qt quatsomes were removed at designated times, serially diluted in PBS, plated on an MHA plate, and incubated for 24 h at 37 • C. After 24 hour's incubation, CFU was counted, converted to log 10 values and plotted on a graph as seen in Equation ( 9) [49]. The electrical conductivity and DNA and protein leakage studies were performed as per previously reported methods [50] and details can be found in the supplementary material, Section S7.…”

Section: Time Killing Assays Vcm-stbaclm-qt Quatsomesmentioning

confidence: 99%

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Formulation of pH-Responsive Quatsomes from Quaternary Bicephalic Surfactants and Cholesterol for Enhanced Delivery of Vancomycin against Methicillin Resistant Staphylococcus aureus

et al. 2020

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Globally, human beings continue to be at high risk of infectious diseases caused by methicillin-resistant Staphylococcus aureus (MRSA); and current treatments are being depleted due to antimicrobial resistance. Therefore, the synthesis and formulation of novel materials is essential for combating antimicrobial resistance. The study aimed to synthesize a quaternary bicephalic surfactant (StBAclm) and thereof to formulate pH-responsive vancomycin (VCM)-loaded quatsomes to enhance the activity of the antibiotic against MRSA. The surfactant structure was confirmed using 1H, 13C nuclear magnetic resonance (NMR), Fourier-transform infrared spectroscopy (FT-IR), and high-resolution mass spectrometry (HRMS). The quatsomes were prepared using a sonication/dispersion method and were characterized using various in vitro, in vivo, and in silico techniques. The in vitro cell biocompatibility studies of the surfactant and pH-responsive vancomycin-loaded quatsomes (VCM-StBAclm-Qt1) revealed that they are biosafe. The prepared quatsomes had a mean hydrodynamic diameter (MHD), polydispersity index (PDI), and drug encapsulation efficiency (DEE) of 122.9 ± 3.78 nm, 0.169 ± 0.02 mV, and 52.22 ± 8.4%, respectively, with surface charge switching from negative to positive at pH 7.4 and pH 6.0, respectively. High-resolution transmission electron microscopy (HR-TEM) characterization of the quatsomes showed spherical vesicles with MHD similar to the one obtained from the zeta-sizer. The in vitro drug release of VCM from the quatsomes was faster at pH 6.0 compared to pH 7.4. The minimum inhibitory concentration (MIC) of the drug loaded quatsomes against MRSA was 32-fold and 8-fold lower at pH 6.0 and pH 7.4, respectively, compared to bare VCM, demonstrating the pH-responsiveness of the quatsomes and the enhanced activity of VCM at acidic pH. The drug-loaded quatsomes demonstrated higher electrical conductivity and a decrease in protein and deoxyribonucleic acid (DNA) concentrations as compared to the bare drug. This confirmed greater MRSA membrane damage, compared to treatment with bare VCM. The flow cytometry study showed that the drug-loaded quatsomes had a similar bactericidal killing effect on MRSA despite a lower (8-fold) VCM concentration when compared to the bare VCM. Fluorescence microscopy revealed the ability of the drug-loaded quatsomes to eradicate MRSA biofilms. The in vivo studies in a skin infection mice model showed that groups treated with VCM-loaded quatsomes had a 13-fold decrease in MRSA CFUs when compared to the bare VCM treated groups. This study confirmed the potential of pH-responsive VCM-StBAclm quatsomes as an effective delivery system for targeted delivery and for enhancing the activity of antibiotics.

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Section: Methodsmentioning

confidence: 99%

Section: Time Killing Assays Vcm-stbaclm-qt Quatsomesmentioning

confidence: 99%

Formulation of pH-Responsive Quatsomes from Quaternary Bicephalic Surfactants and Cholesterol for Enhanced Delivery of Vancomycin against Methicillin Resistant Staphylococcus aureus

et al. 2020

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“…The SLNs alone and marketed silver sulfadiazine cream showed wound area retraction of 95% and 76% after 21 days, respectively. An oleylamine zwitterionic lipid was combined with chitosan to form vancomycin-loaded lipid-polymer nanohybrids for treating MRSA biofilm infections [163]. This lipid had the feature of zwitterionic pH sensitivity.…”

Section: Lipid-based Nanoparticlesmentioning

confidence: 99%

The Antibiofilm Nanosystems for Improved Infection Inhibition of Microbes in Skin

et al. 2021

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Biofilm formation is an important virulence factor for the opportunistic microorganisms that elicit skin infections. The recalcitrant feature of biofilms and their antibiotic tolerance impose a great challenge on the use of conventional therapies. Most antibacterial agents have difficulty penetrating the matrix produced by a biofilm. One novel approach to address these concerns is to prevent or inhibit the formation of biofilms using nanoparticles. The advantages of using nanosystems for antibiofilm applications include high drug loading efficiency, sustained or prolonged drug release, increased drug stability, improved bioavailability, close contact with bacteria, and enhanced accumulation or targeting to biomasses. Topically applied nanoparticles can act as a strategy for enhancing antibiotic delivery into the skin. Various types of nanoparticles, including metal oxide nanoparticles, polymeric nanoparticles, liposomes, and lipid-based nanoparticles, have been employed for topical delivery to treat biofilm infections on the skin. Moreover, nanoparticles can be designed to combine with external stimuli to produce magnetic, photothermal, or photodynamic effects to ablate the biofilm matrix. This study focuses on advanced antibiofilm approaches based on nanomedicine for treating skin infections. We provide in-depth descriptions on how the nanoparticles could effectively eliminate biofilms and any pathogens inside them. We then describe cases of using nanoparticles for antibiofilm treatment of the skin. Most of the studies included in this review were supported by in vivo animal infection models. This article offers an overview of the benefits of nanosystems for treating biofilms grown on the skin.

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“…Among all other intrinsic stimuli‐responsive nanocarriers, pH‐responsive antibacterial nanocarriers remain the most investigated. For instance, to date, the pH‐responsive antibacterial nanocarriers that have been investigated are nanovesicles (Hassan et al, 2020); nanoemulsions (Moradi, Barati, Salehi, Tonelli, & Hamedi, 2019); nanospheres (Mao et al, 2017); nanocapsules (Cai, Wang, & Zhang, 2019; Cao et al, 2019); coacervate‐based nanocarriers (Niaz, Shabbir, Noor, Abbasi, & Imran, 2019); nanosheets (Chahardahmasoumi, Sarvi, & Jalali, 2019); and nanowires (Karuppusamy, Pratheepkumar, Dhandapani, Maruthamuthu, & Kulandainathan, 2015). In addition, an enzyme‐responsive nanocapsule and nanovesicle with antibacterial efficacy have also been reported (Baier et al, 2013; Li et al, 2016).…”

Section: Intrinsic Stimuli‐responsive Nanocarriers For Antibacterial mentioning

confidence: 99%

“…The most recent study was carried out by Hassan et al (2020), in which they synthesized pH‐responsive lipid‐polymer hybrid nanovesicles from a novel oleylamine‐based zwitterionic lipid. This was achieved using the ionic gelation method and vancomycin was incorporated into the vesicles.…”

Section: Intrinsic Stimuli‐responsive Nanocarriers For Antibacterial mentioning

confidence: 99%

Intrinsic stimuli‐responsive nanocarriers for smart drug delivery of antibacterial agents—An in‐depth review of the last two decades

Devnarain

Osman

Fasiku

et al. 2020

WIREs Nanomed Nanobiotechnol

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Antibiotic resistance due to suboptimal targeting and inconsistent antibiotic release at bacterial infection sites has driven the formulation of stimuli‐responsive nanocarriers for antibacterial therapy. Unlike conventional nanocarriers, stimuli‐responsive nanocarriers have the ability to specifically enhance targeting and drug release profiles. There has been a significant escalation in the design and development of novel nanomaterials worldwide; in particular, intrinsic stimuli‐responsive antibiotic nanocarriers, due to their enhanced activity, improved targeted delivery, and superior potential for bacterial penetration and eradication. Herein, we provide an extensive and critical review of pH‐, enzyme‐, redox‐, and ionic microenvironment‐responsive nanocarriers that have been reported in literature to date, with an emphasis on the mechanisms of drug release, the nanomaterials used, the nanosystems constructed and the antibacterial efficacy of the nanocarriers. The review also highlights further avenues of research for optimizing their potential and commercialization. This review confirms the potential of intrinsic stimuli‐responsive nanocarriers for enhanced drug delivery and antibacterial killing. This article is categorized under: Therapeutic Approaches and Drug Discovery > Nanomedicine for Infectious Disease Nanotechnology Approaches to Biology > Nanoscale Systems in Biology

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Novel chitosan-based pH-responsive lipid-polymer hybrid nanovesicles (OLA-LPHVs) for delivery of vancomycin against methicillin-resistant Staphylococcus aureus infections

Cited by 50 publications

References 120 publications

Formulation of pH-Responsive Quatsomes from Quaternary Bicephalic Surfactants and Cholesterol for Enhanced Delivery of Vancomycin against Methicillin Resistant Staphylococcus aureus

Formulation of pH-Responsive Quatsomes from Quaternary Bicephalic Surfactants and Cholesterol for Enhanced Delivery of Vancomycin against Methicillin Resistant Staphylococcus aureus

The Antibiofilm Nanosystems for Improved Infection Inhibition of Microbes in Skin

Intrinsic stimuli‐responsive nanocarriers for smart drug delivery of antibacterial agents—An in‐depth review of the last two decades

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