Novel chitosan‐based hyaluronan hybrid polymer fibers as a scaffold in ligament tissue engineering

Abstract

To clarify the feasibility of using novel chitosan-based hyaluronan hybrid polymer fibers as a scaffold in ligament tissue engineering, their mechanical properties and ability to promote cellular adhesion, proliferation, and extracellular matrix production were studied in vitro. Chitosan fibers and chitosan-based 0.05% and 0.1% hyaluronan hybrid fibers were developed by the wet spinning method. Hyaluronan coating significantly increased mechanical properties, compared to the chitosan fibers. Rabbit fibroblasts adhesion onto hybrid fibers was significantly greater than for the control and chitosan fibers. For analysis of cell proliferation and extracellular matrix production, a three-dimensional scaffold was created by simply piling up each fiber. At 1 day after cultivation, the DNA content in the hybrid scaffolds was higher than that in the chitosan scaffold. Scanning electron microscopy showed that the fibroblasts had produced collagen fibers after 14 days of culture. Immunostaining for type I collagen was clearly predominant in the hybrid scaffolds, and the mRNA level of type I collagen in the hybrid scaffolds were significantly greater than that in the chitosan scaffold. The present study revealed that hyaluronan hybridization with chitosan fibers enhanced fiber mechanical properties and in vitro biological effects on the cultured fibroblasts.

Sorbitol-Plasticized and Neutralized Chitosan Membranes as Skin Substitutes

“…For some cells of specific tissue types, sometimes, chitosan based biomaterials do not provide a good interface for cell interaction 26,27 . Therefore, other biomaterials, such as collagen 28,29 , gelatin 24 , hyaluronan 30 , heparin 31 , silk fibroin 32 , fibrin 33 or poly-D-lysine 34 with tissue-specific binding sequence, could be blended with chitosan to improve cell affinity. For this finality, and improve cell and tissue response, chitosan scaffolds also had been also loaded with many bioactive molecules, such as FGF2, EGF, PDGF, VEFG, IFN-γ or TGF-β1 35 .…”

Development of mature cartilage constructs using novel three‐dimensional porous scaffolds for enhanced repair of osteochondral defects

“…16,18 The detailed information on the current fibers was mentioned in our previous reports. 16,17,19 On the basis of our structural data, 17 sheets with 400-lm pore size were woven automatically from yarns created from the hybrid fibers by using the original apparatus. To achieve engineered constructs with mechanically mature cartilage, we developed two types of novel 3D scaffolds using this sheet and fibers for this study.…”

Silk fibroin/hyaluronan scaffolds for human mesenchymal stem cell culture in tissue engineering

“…The relation between HA composition, bone related genes and extracellular matrix components (e.g. collagen type-I and type-III) seems to be specific for each particular scaffold [25,27,50,53]. Although the (often contradictory) results have been typically linked to a direct effect of HA, it is still questionable whether some of them might be causally related to the unsatisfactory mechanical properties of HA-based scaffolds.…”