Novel characterization of NADC30-like and NADC34-like PRRSV strains in China: epidemiological status and pathogenicity analysis of L1A variants1

Xu, Hu; Zhang, Siyu; Guo, Zhenyang; Li, Chao; Gong, Bangjun; Li, Jinhao; Sun, Qi; Zhao, Jing; Zhang, Menglin; Xiang, Lirun; Tang, Yan-dong; Leng, Chaoliang; Wu, Jianan; Wang, Qian; Peng, Jinmei; Zhou, Guohui; Liu, Huairan; An, Tongqing; Cai, Xuehui; Tian, Zhi-Jun; Zhang, Hongliang

doi:10.1016/j.jia.2024.03.056

Cited by 1 publication

(1 citation statement)

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“…While NADC30-like PRRSV is considered to be of relatively milder virulence compared to HP-PRRSV, it has been associated with clinical respiratory symptoms in all-age pigs and later-term abortion, with a rate of 30-40% in sows [57][58][59]. Despite being less aggressive than HP-PRRSV, the NADC30-like strain and its recombinant strains often show persistent infection with a longer course of disease [60][61][62]. Moreover, current commercial vaccines can only provide limited cross-protection against NADC30-like PRRSV, so it still poses a serious health risk to pig herd [63][64][65].…”

Section: Introductionmentioning

confidence: 99%

Fidelity Characterization of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and NADC30-like Strain

Gao,

Bian,

Gao

et al. 2024

Viruses

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The porcine reproductive and respiratory syndrome virus (PRRSV) has significantly impacted the global pork industry for over three decades. Its high mutation rates and frequent recombination greatly intensifies its epidemic and threat. To explore the fidelity characterization of Chinese highly pathogenic PRRSV JXwn06 and the NADC30-like strain CHsx1401, self-recombination and mutation in PAMs, MARC-145 cells, and pigs were assessed. In vitro, CHsx1401 displayed a higher frequency of recombination junctions and a greater diversity of junction types than JXwn06. In vivo, CHsx1401 exhibited fewer junction types yet maintained a higher junction frequency. Notably, JXwn06 showed more accumulation of mutations. To pinpoint the genomic regions influencing their fidelity, chimeric viruses were constructed, with the exchanged nsp9-10 regions between JXwn06 and CHsx1401. The SJn9n10 strain, which incorporates JXwn06’s nsp9-10 into the CHsx1401 genome, demonstrated reduced sensitivity to nucleotide analogs compared to CHsx1401. Conversely, compared with JXwn06, the JSn9n10 strain showed increased sensitivity to these inhibitors. The swapped nsp9-10 also influences the junction frequency and accumulated mutations as their donor strains. The results indicate a propensity for different types of genetic variations between these two strains and further highlight the nsp9-10 region as a critical determinant of their fidelity.

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