Novel Cell-Ess ® supplement used as a feed or as an initial boost to CHO serum free media results in a significant increase in protein yield and production

Abstract

Many metrics, including metabolic profiles, have been used to analyze cell health and optimize productivity. In this study, we investigated the ability of a lipid supplement to increase protein yield. At a concentration of 1% (v/v) the lipid supplement caused a significant increase in protein titer (1118 ± 65.4 ng 105 cells− 1 days− 1) when compared to cultures grown in the absence of supplementation (819.3 ± 38.1 ng 105 cells− 1 days− 1; p < 0.05). This equated to a 37% increase in productivity. Furthermore, metabolic profiles of ammonia, glutamate, lactate, and glucose were not significantly altered by the polar lipid supplement. In a separate set of experiments, using the supplement as a feed resulted in 2 notable effects. The first was a 25% increase in protein titer. The second was an extension of peak protein production from 1 day to 2 days. These results suggest that lipid supplementation is a promising avenue for enhancing protein production. In addition, our results also suggest that an increase in protein production may not necessarily require a change in the metabolic state of the cells.

Transient CHO expression platform for robust antibody production and its enhanced N‐glycan sialylation on therapeutic glycoproteins

“…GT1 DNA was mixed with the target gene Mab‐F (1 μg/mL) as indicated percentage of 1 μg/mL, and transfected into ExpiCHO‐S™ cells at a scale of 100 mL or 500 mL. UMnG (1×: 1 mM uridine, 0.002 mM MnCl2, 5 mM galactose) and Cell‐Ess were added 24 h posttransfection. Conditioned medium was harvested on Day 4 or Day 7.…”

“…Adding lipid supplement Cell‐Ess to transient ExpiCHO cell culture didn't result in a significant increase in the fractions of G1F species or G2F species in transient ExpiCHO‐derived glycans (Figure B). It has been hypothesized that lipid supplements might affect glycosylation by influencing membrane composition of ER or Golgi and consequentially the distribution of membrane‐anchored glycosyltransferases, the optimal use of lipid supplements might require further condition screening as the lipid supplements are sensitive to the constituents of culture medium …”

“…In addition, it has been reported that increasing GT expression could increase terminal galactosylation of N‐glycans . What is more, a lipid supplement Cell‐Ess is reported to enhance protein yield and might improve N‐glycosylation as well. The authors therefore set out to examine the effect of these strategies on N‐glycan galactosylation.…”

Human Cells Grown With or Without Substitutes for Fetal Bovine Serum

“…Cell-Ess ® was found to be the least effective on our three human cell lines. Based on an earlier report 30 of its inability to maintain immunoglobulin producing cells, Cell-Ess ® may therefore be no better than a feeder or initial booster of monoclonal antibody-producing cells. GroPro ® and FetalGro ® were better, which at least closely compares to the performance of FBS with our endothelial cell lines (at least for the 2-weekly cell passages).…”

“…The totally non-animal product tested was Cell-Ess ® (Essential Pharmaceuticals). Cell-Ess ® is a newer product and only appears in one previous publication 30 , as a feeder booster to increase the protein yield of monoclonal antibody-producing human cells. Another newly marketed product comes from human platelet donors, a lysate product called GroPro ® (Zenbio Inc.).…”