Novel cationic lipids possessing protonated cyclen and imidazolium salt for gene delivery

Huang, Qiao; Ou, Wen; Feng, Zhi-Hua; Wang, Jingyi; Zhang, Ji; Zhu, Wen; Yu, Xiao‐Qi

doi:10.1016/j.ejpb.2011.03.017

Cited by 38 publications

(28 citation statements)

References 32 publications

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“…However, results herein suggest that in the studied type of cationic lipids, diosgenin is much suitable to be the hydrophobic group for high in vitro gene transfection than cholesterol. In addition, different from the aforementioned protonated cyclen-and imidazolium salt-based cationic lipids, which could mediate effective gene transfection only with the presence of calcium ion (Ca 2+ ) (20), the cationic lipids L1 and L2 here could induce effective gene transfection without the calcium ion (Ca 2+ ). In most case, the addition of calcium ion (Ca 2+ ) could promote the uptake of lipoplexes by cells through ion channel mechanism and then increase transfection efficiencies; however, the strategy could not be applied in in vivo gene transfection because of high cytotoxicity induced by Ca 2+ (29).…”

Section: Formation and Characterization Of Liposome ⁄ Dna Complexes (mentioning

confidence: 95%

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Cationic Lipids Containing Cyclen and Ammonium Moieties as Gene Delivery Vectors

Huang¹,

Ren²,

et al. 2012

Chem Biol Drug Des

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These authors contributed equally to this work.In this study, two novel cationic lipids containing protonated cyclen and quaternary ammonium moieties were designed and synthesized as non-viral gene delivery vectors. The structures of the two lipids differ in their hydrophobic region (cholesterol or diosgenin). Cationic liposomes were easily prepared from the lipids individually or from the mixtures of each cationic lipid and dioleoylphosphatidylethanolamine. Several studies including DLS, gel retardation assay, and ethidium bromide intercalation assay suggest that these amphiphilic molecules are able to bind and compact DNA into nanometer particles which can be used as non-viral gene delivery agents. Our results from in vitro transfection show that in association with dioleoylphosphatidylethanolamine, two cationic lipids can induce effective gene transfection in human embryonic kidney 293 cells, although the gene transfection efficiencies of two cationic lipids were found to be lower than that of lipofectamine 2000 TM . Besides, different cytotoxicity was found for two lipoplexes. This study demonstrates that the title cationic lipids have large potential to be efficient non-viral gene vectors.

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Section: Formation and Characterization Of Liposome ⁄ Dna Complexes (mentioning

confidence: 95%

“…These lipids could mediate effective gene transfection in association with neutral lipid dioleoylphosphatidylethanolamine (DOPE) and calcium ion (Ca 2+ ). However, with the absence of calcium ion (Ca 2+ ), the two cationic lipids have nearly no gene transfection abilities (20).…”

mentioning

confidence: 99%

Cationic Lipids Containing Cyclen and Ammonium Moieties as Gene Delivery Vectors

Huang¹,

Ren²,

et al. 2012

Chem Biol Drug Des

Self Cite

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“…The cationic groups commonly used are ammonium [53,54], imidazolium [55][56][57], pyridinium [58,59], lysine or arginine [60][61][62], and/or amine/polyamines [63][64][65][66][67].…”

Section: Cationic Lipids Forming Lipoplexesmentioning

confidence: 99%

Recent progress in gene therapy to deliver nucleic acids with multivalent cationic vectors

Junquera

Aicart

2016

Advances in Colloid and Interface Science

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“…However, guanidino, imidazole, pyridinium, can be present. 35,36 The hydrophobic tails are usually made of two types of hydrophobic moieties, aliphatic chains or steroids. The most common linkages between the hydrophilic head and hydrophobic moieties are ethers, esters, carbamates, or amides.…”

Section: Cationic Lipidsmentioning

confidence: 99%

Synthetic <I>In Vitro</I> Delivery Systems for Plasmid DNA in Eukaryotes

Avila¹,

Lee²,

Tomich³

2014

Journal of Nanopharmaceutics and Drug Delivery

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Success for gene therapy clinical protocols depends on the design of safe and efficient gene carriers. Nature had already designed efficient DNA or RNA delivery devices, namely virus particles. However, they have a propensity to trigger neutralizing and other immune responses and insertional mutagenesis have limited their clinical use. Alternatively, safer approaches involving non-viral carriers have been and continue to be developed although they have not reached the transfection levels achieved by viruses. Those methods can be broadly classified into two categories: chemical and physical methods. In this review we present the most common and recent chemical non-viral methods to introduce, in vitro, pDNA into eukaryotic cells.

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Novel cationic lipids possessing protonated cyclen and imidazolium salt for gene delivery

Cited by 38 publications

References 32 publications

Cationic Lipids Containing Cyclen and Ammonium Moieties as Gene Delivery Vectors

Cationic Lipids Containing Cyclen and Ammonium Moieties as Gene Delivery Vectors

Recent progress in gene therapy to deliver nucleic acids with multivalent cationic vectors

Synthetic <I>In Vitro</I> Delivery Systems for Plasmid DNA in Eukaryotes

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