Novel canine circovirus strains from Thailand: Evidence for genetic recombination

Piewbang, Chutchai; Jo, Wendy K; Puff, Christina; Vries, Erhard van der; Kesdangsakonwut, Sawang; Rungsipipat, Anudep; Kruppa, Jochen; Jung, Klaus; Baumgärtner, Wolfgang; Techangamsuwan, Somporn; Ludlow, Martin; Osterhaus, Albert D. M. E.

doi:10.1038/s41598-018-25936-1

Cited by 55 publications

(78 citation statements)

References 47 publications

(54 reference statements)

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“…Using the RDP package, a total of eight recombination events were detected in the canine CV sequences in this study. Previous studies have shown that recombination events can occur not only in the replicase gene [23] but also in other parts of the genome [22], which is in agreement with our results. In addition, this study also confirmed that intragenotypic and inter-genotypic recombination events have occurred.…”

Section: Discussionsupporting

confidence: 94%

Detection and molecular characterization of canine circovirus circulating in northeastern China during 2014–2016

et al. 2019

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Canine circovirus (canine CV) is an etiological agent associated with diarrhea, hemorrhagic gastroenteritis and vasculitis. Although canine CV has been identified and characterized in southern China in recent years, its epidemiology in other regions of China and its precise molecular characteristics have not been examined. In this study, we examined 141 fecal specimens collected from domestic dogs with or without diarrhea in Heilongjiang province, Northeastern China, during 2014 to 2016. A total of 18 out of 141 samples were found to be positive for canine CV by real-time quantitative PCR. In the diarrhea samples, canine CV was detected in coinfections with canine parvovirus 2. More importantly, two different canine CV strains were detected in one sample. Five canine CV genomes were successfully amplified. Sequence analysis showed that there were two unique amino acid changes in the Rep protein (N39S in the K1 strain, and T71A in the XF16 strain). Phylogenetic analysis indicated that canine CV could be divided into four genotypes, and specific nucleotide mutations could be used for confirming the four genotypes. Moreover, recombination analysis revealed that a total of eight recombination events were found in five genomic sequences. Molecular evolution analysis showed that the canine CV has been under purifying selection. This study provides evidence that at least three genotypes of canine CV are co-circulating in China. Continuous epidemiological surveillance is therefore necessary to understand their importance for the evolution of canine CV.

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Section: Discussionsupporting

confidence: 94%

Detection and molecular characterization of canine circovirus circulating in northeastern China during 2014–2016

et al. 2019

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“…Also, we found the presence of a unique mutation in the capsid protein (I371F) which is present in all Colombian strains sequence. No recombination events were obtained in the circulating CanineCv from Colombia, however previously reported recombination events were found 31 .…”

Section: Resultsmentioning

confidence: 73%

First detection and full genomic analysis of Canine Circovirus in CPV-2 infected dogs in Colombia, South America

Giraldo-Ramirez

Rendón-Marín

Vargas-Bermúdez

et al. 2020

Sci Rep

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Canine Circovirus (CanineCV) is an emerging virus which since its first report in USA in 2012, it has been described worldwide. It was the second mammalian circovirus species identified in dogs and its role in canine enteritis is still being uncertain as much as its association in disease with the Canine Parvovirus-2 (CPV-2). Here, we aim to confirm for the first time the presence of CanineCV in Colombia and to develop phylogenetic evolutive analyses of CanineCV in CPV-2 positive animals. DNA from samples were extracted and PCR, full genome sequencing and phylogenetic analysis was performed to detect and characterize CanineCV. From a total of 30 CPV-2 positive samples, 16.6% (n = 5) were positives for CanineCV. Sequencing analysis of Colombian CanineCV wild-type strains displayed high identity to each other (99.5–99.7% nt; 99.7% aa). The full genome phylogenetic analysis confirmed that worldwide reported CanineCV strains were separated into four distinct genotypes in addition to a European origin of the South American CanineCV strains. This study demonstrated the importance of continue surveillance of emerging viruses in canine populations and confirm for the first time the circulation and origin of CanineCV in Colombia.

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“…The putative recombinant served as a query to detect recombination with their parent lineages that derived from the RDP. The recombination analysis was modeled with a window size of 200 bp and step size of 20 bp 16,39 .…”

Section: Methodsmentioning

confidence: 99%

Genetic and evolutionary analysis of a new Asia-4 lineage and naturally recombinant canine distemper virus strains from Thailand

Piewbang

Radtanakatikanon

Puenpa

et al. 2019

Sci Rep

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Canine distemper virus (CDV), a pathogen causing fatal disease in a wide range of carnivores, can be classified into several geographically-related lineages. It is unclear how genetic recombination contributed to the evolution and emergence of the novel CDV strains and the evolutions of these strains are not fully yet investigated. In this study, the complete genome sequences of eight CDV viruses, isolated from domestic dogs in Thailand, were investigated. Interestingly, most of the identified CDV strains (CDV1-3, -5, -8 TH/2014) clustered as a novel Asia-4 lineage, while the CDV4, -6, -7 TH/2014 belonged to the Asia-1 lineage. Recombination analysis revealed that the CDV4 TH/2014 is a putative recombinant virus from the Asia-1 and America-2 parent viruses. In contrast, no recombination events were detected in the Asia-4 lineage, indicating that it is a distinctive lineage. Evolutionary analysis suggested that the CDV Asia-4 lineage had emerged since 1924 and shared common ancestor with the America-2 lineage. Pressure analysis revealed that CDV nucleotides were under negative selection pressure for its rapid adaptation. These findings demonstrate the evolution of CDV Asia-4 lineage and identified the Asia-1 recombination event. The information regarding genetic diversity of CDVs is essential for further CDV’s research and monitoring.

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Novel canine circovirus strains from Thailand: Evidence for genetic recombination

Cited by 55 publications

References 47 publications

Detection and molecular characterization of canine circovirus circulating in northeastern China during 2014–2016

Detection and molecular characterization of canine circovirus circulating in northeastern China during 2014–2016

First detection and full genomic analysis of Canine Circovirus in CPV-2 infected dogs in Colombia, South America

Genetic and evolutionary analysis of a new Asia-4 lineage and naturally recombinant canine distemper virus strains from Thailand

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