Novel BVDV-2 mutants as new candidates for modified-live vaccines

Zemke, Johanna Marie Luise; König, Patricia; Mischkale, Katrin; Reimann, Ilona; Beer, Martin

doi:10.1016/j.vetmic.2009.09.045

Cited by 15 publications

(14 citation statements)

References 42 publications

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“…Thus, the trigger for the immune system is probably not very strong. Nevertheless, induction of protective immunity has been successfully demonstrated in stringent challenge models in several cases (Frey et al, 2006;Loy et al, 2013;Maurer et al, 2005;Sun et al, 2011;Yang et al, 2012;Zemke, Koenig, Mischkale, Reimann, & Beer, 2010). However, it is not yet clear whether a single vaccination with a replicon can consistently prevent fetal infection in a pregnant animal.…”

Section: Repliconsmentioning

confidence: 99%

The Molecular Biology of Pestiviruses

Tautz

Tews

Meyers

2015

Advances in Virus Research

200

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Section: Repliconsmentioning

confidence: 99%

The Molecular Biology of Pestiviruses

Tautz

Tews

Meyers

2015

Advances in Virus Research

200

View full text Add to dashboard Cite

“…Bovine viral diarrhea virus, a single-stranded RNA is found in cattle and other ruminants worldwide [1-4]. The presence of BVDV in other domestic species such as sheep or wild species such as whitetail deer might be relevant to the epidemiology other disease in cattle [5].…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of a bovine viral diarrhea virus from sika deer in china

Gao

Wang

et al. 2011

Virol J

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BackgroundBovine viral diarrhea virus (BVDV) infections continue to cause significantly losses in the deer population. Better isolation and identification of BVDV from sika deer may contribute significantly to the development of prophylactic therapeutic, and diagnostic reagents as well as help in prevention and control of BVDV. However, isolation and identification of BVDV from sika deer is seldom reported in literature. In this study, we collected some samples according to clinical sign of BVDV to isolation and identification of BVDV from sika deer.Resultswe isolated a suspected BVDV strain from livers of an aborted fetus from sika deer in Changchun (China) using MDBK cell lines, named as CCSYD strain, and identified it by cytopathic effect (CPE), indirect immunoperoxidase test (IPX) and electron microscopy(EM). The results indicated that this virus was BVDV by a series of identification. The structural proteins E0 gene was cloned and sequenced. The obtained E0 gene sequence has been submitted to GenBank with the accession number: FJ555203. Alignment with other 9 strains of BVDV, 7 strains of classical swine fever virus (CSFV) and 3 strains of border disease virus(BDV) in the world, showed that the homology were 98.6%-84.8%, 76.0%-74.7%, 76.6%-77.0% for nucleotide sequence, respectively. The phylogenetic analysis indicated that new isolation and identification CCSYD strain belonged to BVDV1b.ConclusionTo the best of our knowledge, this is the first report that BVDV was isolated and identified in sika deer. This current research contributes development new BVDV vaccine to prevent and control of BVD in sika deer.

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“…There are no commercially available recombinant or vectored vaccines, and thus producers are limited to either modified live MLV or inactivated vaccine approaches [5]. Additionally, some success has been shown experimentally using BVD pseudovirions, which have a deletion in the structural genes, and are rescued using homologous helper RNA in trans [6]. …”

Section: Resultsmentioning

confidence: 99%

Development and evaluation of a replicon particle vaccine expressing the E2 glycoprotein of bovine viral diarrhea virus (BVDV) in cattle

Loy

Gander²,

Mogler

et al. 2013

Virol J

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BackgroundBovine viral diarrhea virus is one of the most significant and costly viral pathogens of cattle worldwide. Alphavirus-derived replicon particles have been shown to be safe and highly effective vaccine vectors against a variety of human and veterinary pathogens. Replicon particles are non-propagating, DIVA compatible, and can induce both humoral and cell mediated immune responses. This is the first experiment to demonstrate that Alphavirus-based replicon particles can be utilized in a standard prime/boost vaccination strategy in calves against a commercially significant bovine pathogen.FindingsReplicon particles that express bovine viral diarrhea virus sub-genotype 1b E2 glycoprotein were generated and expression was confirmed in vitro using polyclonal and monoclonal antibodies specific to E2. Vaccine made from particles was generated in Vero cells and administered to BVDV free calves in a prime/boost regimen at two dosage levels. Vaccination resulted in neutralizing antibody titers that cross-neutralized both type 1 and type 2 BVD genotypes following booster vaccination. Additionally, high dose vaccine administration demonstrated some protection from clinical disease and significantly reduced the degree of leukopenia caused by viral infection.ConclusionsReplicon particle vaccines administered in a prime/boost regimen expressing BVDV E2 glycoprotein can induce cross-neutralizing titers, reduce leukopenia post challenge, and mitigate clinical disease in calves. This strategy holds promise for a safe and effective vaccine to BVDV.

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Novel BVDV-2 mutants as new candidates for modified-live vaccines

Cited by 15 publications

References 42 publications

The Molecular Biology of Pestiviruses

The Molecular Biology of Pestiviruses

Isolation and identification of a bovine viral diarrhea virus from sika deer in china

Development and evaluation of a replicon particle vaccine expressing the E2 glycoprotein of bovine viral diarrhea virus (BVDV) in cattle

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