Novel bisegmented virus (picobirnavirus) of animals, birds and humans

Most human emerging infectious diseases originate from wildlife and bats are a major reservoir of viruses, a few of which have been highly pathogenic to humans. In some regions of Cameroon, bats are hunted and eaten as a delicacy. This close proximity between human and bats provides ample opportunity for zoonotic events. To elucidate the viral diversity of Cameroonian fruit bats, we collected and metagenomically screened eighty-seven fecal samples of Eidolon helvum and Epomophorus gambianus fruit bats. The results showed a plethora of known and novel viruses. Phylogenetic analyses of the eleven gene segments of the first complete bat rotavirus H genome, showed clearly separated clusters of human, porcine, and bat rotavirus H strains, not indicating any recent interspecies transmission events. Additionally, we identified and analyzed a bat bastrovirus genome (a novel group of recently described viruses, related to astroviruses and hepatitis E viruses), confirming their recombinant nature, and provide further evidence of additional recombination events among bat bastroviruses. Interestingly, picobirnavirus-like RNA-dependent RNA polymerase gene segments were identified using an alternative mitochondrial genetic code, and further principal component analyses suggested that they may have a similar lifestyle to mitoviruses, a group of virus-like elements known to infect the mitochondria of fungi. Although identified bat coronavirus, parvovirus, and cyclovirus strains belong to established genera, most of the identified partitiviruses and densoviruses constitute putative novel genera in their respective families. Finally, the results of the phage community analyses of these bats indicate a very diverse geographically distinct bat phage population, probably reflecting different diets and gut bacterial ecosystems.

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“…1988 ; Smits et al. 2011 , 2012 ; Mondal and Majee 2014 ;). PBVs are currently classified into two genogroups ( Smits et al.…”

Section: Double-stranded Rna Viral Sequencesmentioning

confidence: 99%

Cameroonian fruit bats harbor divergent viruses, including rotavirus H, bastroviruses, and picobirnaviruses using an alternative genetic code

et al. 2018

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“…The molecular characterization of the segment two of human and animal strains revealed that PBV are highly variable, and due to these variations, they are divided into two distinct genogroups, genogroup I (GI) and genogroup II (GII) [5][6][7]. The first detection of PBV was from a child's stool sample [8].…”

mentioning

confidence: 99%

Genetic diversity of bovine Picobirnavirus, Brazil

et al. 2018

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Picobirnaviruses (PBVs) are emerging and opportunistic viruses with possible zoonotic potential. In this study, we present the detection, molecular characterization, and genotypic differentiation of PBVs from genogroup I in bovine stool samples from different Brazilian regions. A high proportion of PCR-positive samples (23.4%) was detected in a total of 77 analyzed. Nucleotide identity, alignment, and phylogenetic analyses revealed high diversity among the studied sequences. The results obtained indicate, for the first time, the circulation of bovine PBVs belonging to genogroup I in different Brazilian states, with heterogeneous phylogenetic-clustering profiles.

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“…O prefixo "pico" denota o pequeno diâmetro da partícula viral (35nm) e "birna" refere-se ao genoma que é constituído por dois segmentos, que variam em tamanho desde 2.4 a 2.6 Kbp, perfil longo, e 1.5 a 1.8 Kbp, perfil curto. (MONDAL & MAJEE, 2014). O tamanho dos segmentos genômicos tem sido estimado utilizando-se como referência o perfil de migração eletroforética do genoma de estripes de rotavírus padrão em eletroforese em gel de poliacrilamida (PERERIRA et al, 1988).…”

Section: Caraterísticas Gerais Do Picobirnavirusunclassified

“…A caracterização molecular das estirpes humanas e animais revelou que PBV são altamente variáveis, reconhecendo assim dois genogrupos, Genogrupo I (GI) e Genogrupo II (GII), que foram baseados em diferentes sequências genômicas do segmento 2 (MONDAL & MAJEE, 2014;MALIK et al, 2014;SILVA et al, 2014). A identidade entre o GI e o GII é de aproximadamente 37 a 40% para nucleotídeos e 17 a 28% para a sequência de aminoácidos (BÁYAI et al, 2003 (BHATTACHARYA et al, 2007;VAN LEEUWEN et al,2010).…”

Section: Caraterísticas Gerais Do Picobirnavirusunclassified

Detecção e caracterização moleculares de <i>Picobirnavirus</i> bovino na região centro-sul do Brasil

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Novel bisegmented virus (picobirnavirus) of animals, birds and humans

Cited by 5 publications

References 32 publications

Cameroonian fruit bats harbor divergent viruses, including rotavirus H, bastroviruses, and picobirnaviruses using an alternative genetic code

Cameroonian fruit bats harbor divergent viruses, including rotavirus H, bastroviruses, and picobirnaviruses using an alternative genetic code

Genetic diversity of bovine Picobirnavirus, Brazil

Detecção e caracterização moleculares de <i>Picobirnavirus</i> bovino na região centro-sul do Brasil

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