Novel bioresorbable tricalcium phosphate/polyhydroxyoctanoate (TCP/PHO) composites as scaffolds for bone tissue engineering applications

Cichoń, Ewelina; Haraźna, Katarzyna; Skibiński, Szymon; Witko, Tomasz; Zima, Aneta; Ślósarczyk, A.; Zimowska, Małgorzata; Witko, Małgorzata; Leszczyński, Bartosz; Wróbel, Andrzej; Guzik, Maciej

doi:10.1016/j.jmbbm.2019.06.028

Cited by 22 publications

(25 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The measurements parameters were identical as in previous mechanical studies regarding PHO films [ 21 ]. Contact angles were obtained with Drop Shape Analyzer KRUSS DSA100M optical contact angle measuring instrument (Hamburg, Germany, Gmbh) as described earlier [ 12 ]. The measurements were made in deionized water.…”

Section: Methodsmentioning

confidence: 99%

“…Orthopedic implants made of PLA-calcium-phosphate-glass-fiber composites degrade faster as pure PLA or glass fibers [ 11 ]. The combination of PLA and polyhydroxyoctanoate (PHO) with hydroxyapatite (HA) ceramic and aluminum-calcium-phosphorous oxide ceramic significantly enhances its mechanical properties and can find application in repairing and replacement of soft and hard tissues [ 9 , 12 ]. Both PLA and PHO can be used to produce bone scaffolds [ 13 ] and thanks to their “smart” properties i.e., piezoelectric characteristics of PHO or PLA can act as electro stimulating device without the need for an external power source or any cables [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…For PHO the hydrolysis can also occur. However, its products are less acidogenic than for PLA, as these are mostly dimers of ( R )-3-hydroxyacids (pKa = 4.84 [ 12 ]). Parameters such as the manufacturing technique or solvents used in the extraction process can significantly affect the biocompatibility of materials.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Insights into In Vitro Wound Closure on Two Biopolyesters—Polylactide and Polyhydroxyoctanoate

et al. 2020

Self Cite

View full text Add to dashboard Cite

Two bio-based polymers have been compared in this study, namely: polylactide (PLA) and polyhydroxyoctanoate (PHO). Due to their properties such as biocompatibility, and biointegrity they are considered to be valuable materials for medical purposes, i.e., creating scaffolds or wound dressings. Presented biopolymers were investigated for their impact on cellular migration strategies of mouse embryonic fibroblasts (MEF) 3T3 cell line. Advanced microscopic techniques, including confocal microscopy and immunofluorescent protocols, enabled the thorough analysis of the cell shape and migration. Application of wound healing assay combined with dedicated software allowed us to perform quantitative analysis of wound closure dynamics. The outcome of the experiments demonstrated that the wound closure dynamics for PLA differs from PHO. Single fibroblasts grown on PLA moved 1.5-fold faster, than those migrating on the PHO surface. However, when a layer of cells was considered, the wound closure was by 4.1 h faster for PHO material. The accomplished work confirms the potential of PLA and PHO as excellent candidates for medical applications, due to their properties that propagate cell migration, vitality, and proliferation—essential cell processes in the healing of damaged tissues.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Insights into In Vitro Wound Closure on Two Biopolyesters—Polylactide and Polyhydroxyoctanoate

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…It has to be noted that PHO also undergoes hydrolysis in similar environments. Nevertheless, its products of degradation are far less acidogenic than lactic acid; PHO degrades to predominantly dimers of ( R )-3-hydroxyacids and in a lower quantity to ( R )-3-hydroxyoctanoic acid (pK a = 4.84) [ 39 ]. Thus, the microenvironment of the cell is less affected by the pH change.…”

Section: Discussionmentioning

confidence: 99%

Vimentin Association with Nuclear Grooves in Normal MEF 3T3 Cells

Feliksiak

Witko

Solarz

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

Vimentin, an intermediate filament protein present in leukocytes, blood vessel endothelial cells, and multiple mesenchymal cells, such as mouse embryonic fibroblasts (MEF 3T3), is crucial for various cellular processes, as well as for maintaining the integrity and durability (stability) of the cell cytoskeleton. Vimentin intermediate filaments (VIFs) adhere tightly to the nucleus and spread to the lamellipodium and tail of the cell, serving as a connector between the nucleus, and the cell’s edges, especially in terms of transferring mechanical signals throughout the cell. How these signals are transmitted exactly remains under investigation. In the presented work, we propose that vimentin is involved in that transition by influencing the shape of the nucleus through the formation of nuclear blebs and grooves, as demonstrated by microscopic observations of healthy MEF (3T3) cells. Grooved, or “coffee beans” nuclei, have, to date, been noticed in several healthy cells; however, these structures are especially frequent in cancer cells—they serve as a significant marker for recognition of multiple cancers. We observed 288 MEF3T3 cells cultured on polyhydroxyoctanoate (PHO), polylactide (PLA), and glass, and we identified grooves, coaligned with vimentin fibers in the nuclei of 47% of cells cultured on PHO, 50% of cells on glass, and 59% of cells growing on PLA. We also observed nuclear blebs and associated their occurrence with the type of substrate used for cell culture. We propose that the higher rate of blebs in the nuclei of cells, cultured on PLA, is related to the microenvironmental features of the substrate, pH in particular.

show abstract

“…Incorporating nanoparticles into PHA polymer expands the potential application area thanks to the significant enhancement of materials properties. The most commonly used nanofillers are silylated kaolinite (Zhang et al, 2009), carbon nanotubes (Misra et al, 2010), bioactive glass (Misra et al, 2007), nanoclay (Bordes et al, 2009), cellulose nanocrystals (Yu et al, 2011), calcium phosphates (Cichoń et al, 2019), and modified hydroxides (Dagnon et al, 2009).…”

Section: Processing and Modifications Of Polyhydroxyalkanoate Polymersmentioning

confidence: 99%

What Has Been Trending in the Research of Polyhydroxyalkanoates? A Systematic Review

Guzik

Witko

Steinbüchel

et al. 2020

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

Over the past decades, enormous progress has been achieved with regard to research on environmentally friendly polymers. One of the most prominent families of such biopolymers are bacterially synthesized polyhydroxyalkanoates (PHAs) that have been known since the 1920s. However, only as recent as the 1990s have extensive studies sprung out exponentially in this matter. Since then, different areas of exploration of these intriguing materials have been uncovered. However, no systematic review of undertaken efforts has been conducted so far. Therefore, we have performed an unbiased search of up-to-date literature to reveal trending topics in the research of PHAs over the past three decades by data mining of 2,227 publications. This allowed us to identify eight past and current trends in this area. Our study provides a comprehensive review of these trends and speculates where PHA research is heading.

show abstract

Novel bioresorbable tricalcium phosphate/polyhydroxyoctanoate (TCP/PHO) composites as scaffolds for bone tissue engineering applications

Cited by 22 publications

References 41 publications

Insights into In Vitro Wound Closure on Two Biopolyesters—Polylactide and Polyhydroxyoctanoate

Insights into In Vitro Wound Closure on Two Biopolyesters—Polylactide and Polyhydroxyoctanoate

Vimentin Association with Nuclear Grooves in Normal MEF 3T3 Cells

What Has Been Trending in the Research of Polyhydroxyalkanoates? A Systematic Review

Contact Info

Product

Resources

About