Novel bioprinting method using a pectin based bioink

Banks, Amanda; Guo, Xiaoru; Chen, Junhong; Kumpaty, Subha; Zhang, Wujie

doi:10.3233/thc-160764

Cited by 35 publications

(20 citation statements)

References 8 publications

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“…Heat-induced gelation of Pluronic F-127 only occurred in the first few layers of the printed scaffold; the rest of the scaffold was gelled with calcium. 6 Using oligochitosan as a novel cross-linker was found to be ineffective, unfortunately, as scaffolds quickly reverted to viscous liquid state (Figure 1(a)). It is thought that oligochitosan did not cross-link the pectin in the same manner as Ca 2+ .…”

Section: Resultsmentioning

confidence: 99%

“…4,5 During a previous study, a novel pectin-based bio-ink and corresponding printing method was developed. 6 Pectin shows similar properties to alginate, a common polysaccharide used for constructing scaffolds, but is preferable to alginate, which may lead to allergic reactions. 7 Pectin is a natural biopolymer found in citrus rinds and is composed of galacturonic acid with carboxyl groups.…”

Section: Introductionmentioning

confidence: 99%

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Stability improvement and characterization of bioprinted pectin-based scaffold

Stealey

Guo

Ren

et al. 2019

Journal of Applied Biomaterials & Functional Materials

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Purpose: Bioprinting is an alternative method for constructing tissues/organs for transplantation. This study investigated the cross-linker influence and post-printing modification using oligochitosan and chitosan for stability improvement. Methods: Oligochitosan was tested as a novel cross-linker to replace Ca 2+ for pectin-based bio-ink. Oligochitosan (2 kD) and different molecular weight of chitosan were used to modify the bioprinted scaffold. Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) were used to characterize the scaffolds. Results: Oligochitosan failed to serve as a viable cross-linker. Successful post-printing modification was confirmed by FTIR and SEM analyses. Conclusion: Regarding post-modification, chitosan-treated scaffolds showed enhanced stability compared to untreated scaffolds. In particular, scaffolds modified with 150 kD chitosan exhibited the highest stability.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Stability improvement and characterization of bioprinted pectin-based scaffold

Stealey

Guo

Ren

et al. 2019

Journal of Applied Biomaterials & Functional Materials

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“…Tan δ was <1 for each hydrogel formulation, thus indicating the formation of solid-like gels, in agreement with previously reported results 28 . Complex viscosity decreased for all hydrogels with increasing oscillation frequency, indicating a shear thinning behavior (Figure 2b), typical of soft materials with viscoelastic properties 43,44 . Storage modulus and complex viscosity were significantly higher for Pe0Ca than for hydrogels produced at increased pH of pectin solutions.…”

Section: Rheological Characterizationmentioning

confidence: 96%

Polysaccharide-based hydrogels with tunable composition as 3D cell culture systems

et al. 2018

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Background: To date, cell cultures have been created either on 2-dimensional (2D) polystyrene surfaces or in 3-dimensional (3D) systems, which do not offer a controlled chemical composition, and which lack the soft environment encountered in vivo and the chemical stimuli that promote cell proliferation and allow complex cellular behavior. In this study, pectin-based hydrogels were developed and are proposed as versatile cell culture systems. Methods: Pectin-based hydrogels were produced by internally crosslinking pectin with calcium carbonate at different initial pH, aiming to control crosslinking kinetics and degree. Additionally, glucose and glutamine were added as additives, and their effects on the viscoelastic properties of the hydrogels and on cell viability were investigated. Results: Pectin hydrogels showed in high cell viability and shear-thinning behavior. Independently of hydrogel composition, an initial swelling was observed, followed by a low percentage of weight variation and a steady-state stage. The addition of glucose and glutamine to pectin-based hydrogels rendered higher cell viability up to 90%-98% after 1 hour of incubation, and these hydrogels were maintained for up to 7 days of culture, yet no effect on viscoelastic properties was detected. Conclusions: Pectin-based hydrogels that offer tunable composition were developed successfully. They are envisioned as synthetic extracellular matrix (ECM) either to study complex cellular behaviors or to be applied as tissue engineering substitutes.

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“…This can be explained by different cross-linking mechanisms of Ca 2+ and oligochitosan. Ca 2+ cross-linking leads to the formation of macromolecular aggregates (egg-box structure) through the intermolecular chelating bonding, while oligochitosan cross-linking forms the pectin-oligochitosan electrolyte complexes (Feng et al 2017;Zhang et al 2016). …”

Section: Effect Of Ca 2+ and Oligochitosan On Cross-liking Of Pectin-mentioning

confidence: 99%

“…Due to its biocompatibility and biodegradability, pectin has been used for various biological applications: including cell encapsulation, creating artificial red blood cells, bioprinting, and drug delivery (Banks et al 2016;Crouse et al 2015;Harvestine et al 2014;Zhang et al 2016a;Zhang et al 2016b). Very recently, pectin has been explored for fabricating nanofibers.…”

Section: Introductionmentioning

confidence: 99%

Electrospinning pectin-based nanofibers: a parametric and cross-linker study

et al. 2018

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Pectin, a natural biopolymer mainly derived from citrus fruits and apple peels, shows excellent biodegradable and biocompatible properties. This study investigated the electrospinning of pectin-based nanofibers. The parameters, pectin:PEO (polyethylene oxide) ratio, surfactant concentration, voltage, and flow rate, were studied to optimize the electrospinning process for generating the pectin-based nanofibers. Oligochitosan, as a novel and nonionic cross-liker of pectin, was also researched. Nanofibers were characterized by using AFM, SEM, and FTIR spectroscopy. The results showed that oligochitosan was preferred over Ca 2+ because it cross-linked pectin molecules without negatively affecting the nanofiber morphology. Moreover, oligochitosan treatment produced a positive surface charge of nanofibers, determined by zeta potential measurement, which is desired for tissue engineering applications.

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Novel bioprinting method using a pectin based bioink

Cited by 35 publications

References 8 publications

Stability improvement and characterization of bioprinted pectin-based scaffold

Stability improvement and characterization of bioprinted pectin-based scaffold

Polysaccharide-based hydrogels with tunable composition as 3D cell culture systems

Electrospinning pectin-based nanofibers: a parametric and cross-linker study

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