Novel biomaterials to study neural stem cell mechanobiology and improve cell-replacement therapies

Kang, Phillip H.; Kumar, Sanjay; Schaffer, David V.

doi:10.1016/j.cobme.2017.09.005

Cited by 20 publications

(12 citation statements)

References 81 publications

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“…Physical characteristics of the engineered environments include stiffness, morphology, and elasticity. Chemical characteristics include hydrophilicity and hydrophobicity and involve chemical moieties that may significantly affect cellular pathways and processes [12,13]. Various materials of different forms and chemical compositions, including polymer/inorganic thin films, composite scaffolds, hydrogels, and fibrous scaffolds, have been constructed as cell culture substrates to control mammalian cells.…”

Section: Engineered Materialsmentioning

confidence: 99%

Molecular-Level Interactions between Engineered Materials and Cells

Jang

Jin

Shankar

et al. 2019

IJMS

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Various recent experimental observations indicate that growing cells on engineered materials can alter their physiology, function, and fate. This finding suggests that better molecular-level understanding of the interactions between cells and materials may guide the design and construction of sophisticated artificial substrates, potentially enabling control of cells for use in various biomedical applications. In this review, we introduce recent research results that shed light on molecular events and mechanisms involved in the interactions between cells and materials. We discuss the development of materials with distinct physical, chemical, and biological features, cellular sensing of the engineered materials, transfer of the sensing information to the cell nucleus, subsequent changes in physical and chemical states of genomic DNA, and finally the resulting cellular behavior changes. Ongoing efforts to advance materials engineering and the cell–material interface will eventually expand the cell-based applications in therapies and tissue regenerations.

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Section: Engineered Materialsmentioning

confidence: 99%

Molecular-Level Interactions between Engineered Materials and Cells

Jang

Jin

Shankar

et al. 2019

IJMS

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“…By studying the biomaterials composition influence on stem cell transplantation therapy, it has been observed that material stiffness confers stem cells’ different properties. Stiffer substrates direct NSCs to astrocyte differentiation, while softer substrates lead NSCs to neurogenic differentiation [ 191 , 192 ]. Chemical composition proves essential for potential beneficial outcomes, particularly in injectable hydrogels development [ 192 ].…”

Section: Remodeling Of the Stroke Tissue Microenvironment Within The Brainmentioning

confidence: 99%

Combination of Stem Cells and Rehabilitation Therapies for Ischemic Stroke

et al. 2021

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Stem cell transplantation with rehabilitation therapy presents an effective stroke treatment. Here, we discuss current breakthroughs in stem cell research along with rehabilitation strategies that may have a synergistic outcome when combined together after stroke. Indeed, stem cell transplantation offers a promising new approach and may add to current rehabilitation therapies. By reviewing the pathophysiology of stroke and the mechanisms by which stem cells and rehabilitation attenuate this inflammatory process, we hypothesize that a combined therapy will provide better functional outcomes for patients. Using current preclinical data, we explore the prominent types of stem cells, the existing theories for stem cell repair, rehabilitation treatments inside the brain, rehabilitation modalities outside the brain, and evidence pertaining to the benefits of combined therapy. In this review article, we assess the advantages and disadvantages of using stem cell transplantation with rehabilitation to mitigate the devastating effects of stroke.

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“…160 For example, various studies have demonstrated that softer substrates can promote neurogenic differentiation in NSCs, while stiffer substrates will instead direct them towards astrocyte differentiation. 161 This highlights the need to optimize these biomaterials for stem cell viability, differentiation, and proliferation. A recent meta-analysis found that biomaterialbased interventions have led to lesion volume reduction and improved neurological outcomes in stroke rodent models.…”

Section: Biomaterialsmentioning

confidence: 99%

“…Additionally, these scaffolds may intrinsically contain properties that can provide cues to stem cells such as matrix topology, mechanical forces, and biochemical properties [ 160 ]. For example, various studies have demonstrated that softer substrates can promote neurogenic differentiation in NSCs, while stiffer substrates will instead direct them towards astrocyte differentiation [ 161 ]. This highlights the need to optimize these biomaterials for stem cell viability, differentiation, and proliferation.…”

Section: Improvement Of Stem Cell Application In Ischemic Brain Injurmentioning

confidence: 99%

Optimizing Stem Cell Therapy after Ischemic Brain Injury

et al. 2020

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Stem cells have been used for regenerative and therapeutic purposes in a variety of diseases. In ischemic brain injury, preclinical studies have been promising, but have failed to translate results to clinical trials. We aimed to explore the application of stem cells after ischemic brain injury by focusing on topics such as delivery routes, regeneration efficacy, adverse effects, and <i>in vivo</i> potential optimization. PUBMED and Web of Science were searched for the latest studies examining stem cell therapy applications in ischemic brain injury, particularly after stroke or cardiac arrest, with a focus on studies addressing delivery optimization, stem cell type comparison, or translational aspects. Other studies providing further understanding or potential contributions to ischemic brain injury treatment were also included. Multiple stem cell types have been investigated in ischemic brain injury treatment, with a strong literature base in the treatment of stroke. Studies have suggested that stem cell administration after ischemic brain injury exerts paracrine effects via growth factor release, blood-brain barrier integrity protection, and allows for exosome release for ischemic injury mitigation. To date, limited studies have investigated these therapeutic mechanisms in the setting of cardiac arrest or therapeutic hypothermia. Several delivery modalities are available, each with limitations regarding invasiveness and safety outcomes. Intranasal delivery presents a potentially improved mechanism, and hypoxic conditioning offers a potential stem cell therapy optimization strategy for ischemic brain injury. The use of stem cells to treat ischemic brain injury in clinical trials is in its early phase; however, increasing preclinical evidence suggests that stem cells can contribute to the down-regulation of inflammatory phenotypes and regeneration following injury. The safety and the tolerability profile of stem cells have been confirmed, and their potent therapeutic effects make them powerful therapeutic agents for ischemic brain injury patients.

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Novel biomaterials to study neural stem cell mechanobiology and improve cell-replacement therapies

Cited by 20 publications

References 81 publications

Molecular-Level Interactions between Engineered Materials and Cells

Molecular-Level Interactions between Engineered Materials and Cells

Combination of Stem Cells and Rehabilitation Therapies for Ischemic Stroke

Optimizing Stem Cell Therapy after Ischemic Brain Injury

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