Novel biological and possible applicable roles of LH/hCG receptor

Ziȩcik, Adam J.; Kaczmarek, Monika M.; Blitek, Agnieszka; Kowalczyk, Anna; Li, Xiangdong; Rahman, Nafis

doi:10.1016/j.mce.2006.08.016

Cited by 56 publications

(46 citation statements)

References 95 publications

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“…LH receptors are expressed in human fallopian tubes and endometrium, and hormonal regulation of LH receptors may be involved in gamete/embryo transport, fallopian tube contraction, and uterine growth and relaxation (48). Thus, further studies are needed to elucidate the mechanisms of the different regulation of hnRNPs by LH.…”

Section: E1276 Hnrnps In the Human Fallopian Tube And Endometriummentioning

confidence: 99%

Coordinate regulation of heterogeneous nuclear ribonucleoprotein dynamics by steroid hormones in the human fallopian tube and endometrium in vivo and in vitro

Shao

Wang

Weijdegård

et al. 2012

American Journal of Physiology-Endocrinology and Metabolism

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Heterogeneous nuclear ribonucleoproteins (hnRNPs), which are chromatin-associated RNA-binding proteins, participate in mRNA stability, transport, intracellular localization, and translation by acting as transacting factors. Several studies have shown that steroid hormones can regulate hnRNP expression. However, to date, the regulation of hnRNPs and their interactions with steroid hormone signaling in fallopian tubes and endometrium are not fully elucidated. In the present study, we determined whether hnRNP expression is regulated during the menstrual cycle and correlates with estrogen receptor (ER) and progesterone receptor (PR) levels in human fallopian tubes in vivo. Because of the limited availability of human tubal tissues for the research, we also explored the mechanisms of hnRNP regulation in human endometrium in vitro. Fallopian tissue was obtained from patients in the early, late, and postovulatory phases and the midsecretory phase and endometrial tissue from premenopausal and postmenopausal women undergoing hysterectomy. We measured expression of hnRNPs and assessed their intracellular localization and interactions with ERs and PRs. We also determined the effects of human chorionic gonadotropin, 17β-estradiol (E2), and progesterone (P4) on hnRNP expression. In fallopian tubes, mRNA and protein levels of hnRNP A1, AB, D, G, H, and U changed dynamically during ovulation and in the midsecretory phase. In coimmunolocation and coimmunoprecipitation experiments, hnRNPs interacted with each other and with ERs and PRs in fallopian tubes. After treatment with E2 and/or P4 to activate ERs and PRs, hnRNP A1, AB, D, G, and U proteins displayed overlapping but distinct patterns of regulation in the endometrium in vitro. Our findings expand the physiological repertoire of hnRNPs in human fallopian tubes and endometrium and suggest that steroid hormones regulate different hnRNPs directly by interacting with ERs and/or PRs or indirectly by binding other hnRNPs. Both actions may contribute to regulation of gene transcription.

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Section: E1276 Hnrnps In the Human Fallopian Tube And Endometriummentioning

confidence: 99%

Coordinate regulation of heterogeneous nuclear ribonucleoprotein dynamics by steroid hormones in the human fallopian tube and endometrium in vivo and in vitro

Shao

Wang

Weijdegård

et al. 2012

American Journal of Physiology-Endocrinology and Metabolism

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“…Estrogen has been shown to stimulate proliferation of the OSE, but it is more likely that hormones play a role in progression of ovarian cancer rather than initiation (58). As the FTE expresses receptors for estradiol, FSH, and LH, it is likely that if hormones play a role in progression of ovarian cancer they would also impact those originating in the fallopian tube (59,60). Studies utilizing DNA damaging agents, such as N-methyl-N'-nitrosourea (MNU) or 7,12-dimethylbenz[a]anthracene (DMBA) attempt to mimic the types of random DNA damage that may occur following repeated iterations of the tear-and-repair process.…”

Section: Ovarian Carcinoma Induced By Carcinogensmentioning

confidence: 99%

Evaluating the progenitor cells of ovarian cancer: analysis of current animal models

King

Burdette

2011

BMB Reports

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“…The expression level of non-gonadal hCG receptors is much lower than in the gonads, although their expression is regulated by similar mechanisms and observed in malignancies (20).…”

Section: Resultsmentioning

confidence: 64%

Development of a radiolabeled β-human chorionic gonadotropin

Jalilian¹,

Khoshdel²,

Garousi³

et al. 2009

Acta Pharmaceutica

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Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced in pregnancy by the developing embryo after conception and later by the syncytiotrophoblast to prevent disintegration of the corpus luteum of the ovary. hCG also plays a role in cellular differentiation/proliferation and may activate apoptosis (1). hCG is extensively used as a parenteral fertility medication in lieu of luteinizing hormone.hCG is composed of 244 amino acids with a molecular mass of 36.7 kDa. It is heterodimeric, with a (alpha) and b (beta) subunits creating a small hydrophobic core. The vast majority of the outer amino acids are hydrophilic.hCG has been radiolabeled mostly with 125 I for various applications, such as kinetic studies of hCG conformations (2), thermodynamic analysis of hCG monoclonal antibodies (3), structure-activity studies (4) and placental protein synthesis studies (5). b-Human chorionic gonadotropin (b-hCG) was successively labeled with [ 67 Ga]-gallium chloride after conjugation with freshly prepared diethylenetriaminepentaacetic acid dianhydride (ccDTPA). After solid phase purification of the radiolabeled hormone, high performance liquid chromatography showed radiochemical purity higher than 95 % under optimized conditions (specific activity = 22-23 TBq mM -1 , labeling efficiency 80 %). Preliminary in vivo studies (ID g -1 , %) in male wild-type rats showed marked gonadal uptake of the tracer after 240 minutes in agreement with the biodistribution studies and reported b-hCG receptors. Target to blood ratios were 5.1 and 15.2 after 3 and 24 hours, respectively, while target to muscle ratios were 35 and 40 after 3 and 24 hours, respectively.

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Novel biological and possible applicable roles of LH/hCG receptor

Cited by 56 publications

References 95 publications

Coordinate regulation of heterogeneous nuclear ribonucleoprotein dynamics by steroid hormones in the human fallopian tube and endometrium in vivo and in vitro

Coordinate regulation of heterogeneous nuclear ribonucleoprotein dynamics by steroid hormones in the human fallopian tube and endometrium in vivo and in vitro

Evaluating the progenitor cells of ovarian cancer: analysis of current animal models

Development of a radiolabeled β-human chorionic gonadotropin

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