Novel bioactive parathyroid hormone and related peptides in teleost fish

Canário, Adelino V.M.; Rotllant, Josep; Fuentes, Juan; Guerreiro, Pedro M.; Teodósio, Rita; Power, Deborah M.; Clark, Melody S.

doi:10.1016/j.febslet.2005.12.023

Cited by 50 publications

(62 citation statements)

References 50 publications

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“…Rotllant et al [46] found that the parathyroid hormone-related protein increased osteoclastic activity in seawater fish scales via cAMP/AC, and Canario et al [47] found that PTH1 or PTH2 failed to activate the cAMP/AC pathway or stimulate calcium transport in seawater fish scales. Notwithstanding these differences, it is known that the exchange of calcium in the scales of freshwater teleosts is faster than that in marine teleosts because freshwater teleosts live in a low-calcium environment.…”

Section: Discussionmentioning

confidence: 99%

Parathyroid hormone 1 (1–34) acts on the scales and involves calcium metabolism in goldfish

Suzuki

Danks

Maruyama

et al. 2011

Bone

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The effect of fugu parathyroid hormone 1 (fugu PTH1) on osteoblasts and osteoclasts in teleosts was examined with an assay system using teleost scale and the following markers: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Synthetic fugu PTH1 (1-34) (100 pg/ml to 10 ng/ml) significantly increased ALP activity at 6 h of incubation. High-dose (10 ng/ml) fugu PTH1 significantly increased ALP activity even after 18 h of incubation. In the case of TRAP activity, fugu PTH1 did not change at 6 h of incubation, but fugu PTH1 (100 pg/ml to 10 ng/ml) significantly increased TRAP activity at 18 h. Similar results were obtained for human PTH (1-34), but there was an even greater response with fugu PTH1 than with human PTH. In vitro, we demonstrated that both the receptor activator of the NF-B ligand in osteoblasts and the receptor activator NF-B mRNA expression in osteoclasts increased significantly by fugu PTH1 treatment. In an in vivo experiment, fugu PTH1 induced hypercalcemia resulted from the increase of both osteoblastic and osteoclastic activities in the scale as well as the decrease of scale calcium contents after fugu PTH1 injection. In addition, an in vitro experiment with intramuscular autotransplanted scale indicated that the ratio of multinucleated osteoclasts/mononucleated osteoclasts in PTH-treated scales was significantly higher than that in the control scales. Thus, we concluded that PTH acts on osteoblasts and osteoclasts in the scales and regulates calcium metabolism in goldfish.3

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Section: Discussionmentioning

confidence: 99%

Parathyroid hormone 1 (1–34) acts on the scales and involves calcium metabolism in goldfish

Suzuki

Danks

Maruyama

et al. 2011

Bone

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“…Indeed, only very recently the genes for parathyroid hormone (PTH), which is the dom inant hypercalcemic factor for terrestrial vertebrates, were found in fish (Danks et al, 1993). However, earlier, fish were shown to express genes for parathyroid hor mone related protein (PTHrP; Power et al, 2000;Flana gan et al, 2000;Canario et al, 2006). PTHrP behaves in fish as a hypercalcemic hormone and appears key in fish calcium physiology (Ingleton et al, 2002;Trivett et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Melatonin synthesis under calcium constraint in gilthead sea bream (Sparus auratus L.)

Abbink

Kulczykowska

Kalamarz

et al. 2008

General and Comparative Endocrinology

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Brain or blood plasma melatonin was analysed as a measure for pineal melatonin production in sea bream. Access to calcium was limited by diluting the seawater to 2.5& and removing calcium from the diet or by prolonged feeding of vitamin D-deficient diet. Inter actions/relations between melatonin and calcium balance and the hypercalcemic endocrines PTHrP and calcitriol were assessed. Restrict ing calcium availability in both water and diet had no effect on plasma melatonin, but when calcium was low in the water or absent from food, increased and decreased plasma melatonin was observed, respectively. Fish on a vitamin D-deficient diet (D-fish) showed decreased plasma calcitriol levels and remained normocalcemic. Decreased brain melatonin was found at all sampling times (10-22 weeks) in the D -fish compared to the controls. A positive correlation between plasma Ca2+ and plasma melatonin was found (R2 = 0.19; N = 41; P < 0.01) and brain melatonin was negatively correlated with plasma PTHrP (R2 = 0.78; N = 4; P < 0.05). The posi tive correlation between plasma levels of melatonin and Ca2+ provides evidence that melatonin synthesis is influenced by plasma Ca2+. The decreased melatonin production in the D -fish points to direct or indirect involvement of calcitriol in melatonin synthesis by the pineal organ in teleosts. The hypercalcemic factors PTHrP and calcitriol appeared to be negatively correlated with melatonin and this substantiates an involvement of melatonin in modulating the endocrine response to cope with hypocalcemia. It further points to the importance of Ca2+ in melatonin physiology.

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“…Another possibility is that elephant shark Pth2 is an orthologue of a Pth-like gene identified in fugu. (30) Canario and colleagues (30) identified a…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, fugu Pth-L does not contain this cleavage site, and the intron is present before an SRR motif. (30) More important, fugu Pth-L is capable of stimulating adenylate cyclase in sea bream scales, (30) whereas the elephant shark (29) suggesting that Pth2 may have a role in neurotransmission, in the neuroendocrine system, or as a neuromodulator. The presence of elephant shark Pth2 mRNA and protein in the spleen implies that it might have a role in the hemopoietic and/or immune systems.…”

Section: Discussionmentioning

confidence: 99%

Parathyroid hormone gene family in a cartilaginous fish, the elephant shark (Callorhinchus milii)

Liu¹,

Ibrahim²,

Tay³

et al. 2010

Journal of Bone and Mineral Research

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The development of bone was a major step in the evolution of vertebrates. A bony skeleton provided structural support and a calcium reservoir essential for the movement from an aquatic to a terrestrial environment. Cartilaginous fishes are the oldest living group of jawed vertebrates. In this study we have identified three members of the parathyroid hormone (Pth) gene family in a cartilaginous fish, the elephant shark (Callorhinchus milii). The three genes include two Pth genes, designated as Pth1 and Pth2, and a Pthrp gene. Phylogenetic analysis suggested that elephant shark Pth2 is an ancient gene whose orthologue is lost in bony vertebrates. The Pth1 and Pth2 genes have the same structure as the Pth gene in bony vertebrates, whereas the structure of the Pthrp gene is more complex in tetrapods compared with elephant shark. The three elephant shark genes showed distinct patterns of expression, with Pth2 being expressed only in the brain and spleen. This contrasts with localization of the corresponding proteins, which showed considerable overlap in their distribution. There were conserved sites of localization for Pthrp between elephant shark and mammals, including tissues such as kidney, skin, skeletal and cardiac muscle, pancreas, and cartilage. The elephant shark Pth1(1-34) and Pthrp(1-34) peptides were able to stimulate cAMP accumulation in mammalian UMR106.01 cells. However, Pth2(1-34) peptide did not show such PTH-like biologic activity. The presence of Pth and Pthrp genes in the elephant shark indicates that these genes played fundamental roles before their recruitment to bone development in bony jawed vertebrates. ß

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Novel bioactive parathyroid hormone and related peptides in teleost fish

Cited by 50 publications

References 50 publications

Parathyroid hormone 1 (1–34) acts on the scales and involves calcium metabolism in goldfish

Parathyroid hormone 1 (1–34) acts on the scales and involves calcium metabolism in goldfish

Melatonin synthesis under calcium constraint in gilthead sea bream (Sparus auratus L.)

Parathyroid hormone gene family in a cartilaginous fish, the elephant shark (Callorhinchus milii)

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