Novel bacterial selenite reductase CsrF responsible for Se(IV) and Cr(VI) reduction that produces nanoparticles in Alishewanella sp. WH16-1

Xia, Xian; Wu, Sha; Li, Nuohan; Wang, Dan; Zheng, Shixue

doi:10.1016/j.jhazmat.2017.08.051

Cited by 95 publications

(67 citation statements)

References 47 publications

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“…In addition, Lampis et al [ 28 ] found that the selenite reduction activity of S. maltophilia SeITE02 cells was mainly attributed to the cytoplasmic fraction with NADH served as an electron donor. Thus, it is possible to infer that selenite that imported into the A. faecalis Se03 cell was reduced and formed inside as small Se 0 seeds in the cytoplasm by thioredoxin reductase [ 39 ], or NADH/NADPH related reductases [ 40 ]. Interestingly, selenium nanoparticles accumulated extracellularly to a noticeable extent after 36 h incubation as shown in TEM micrograph ( Figure 4 B), suggesting the SeNPs formed inside the cell could be released into the extracellular space through certain export mechanism.…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies have found that selenite can react with glutathione (GSH) to form Se 0 and reduced GSH through Painter-type reactions [ 46 ] and that other enzymatic systems, such as sulfite reductase, flavoprotein, and thioredoxin, are also involved in the reduction of selenite and Se 0 formation [ 40 , 47 ]. Therefore, the mRNA expression of genes suspected to be involved in selenite reduction and SeNP formation in A. faecalis Se03, including glutathione synthetase ( gsh B), sulfite reductase ( Cys I), the sulfate transporter subunit ( BV899_03375 ), flavoprotein sulfite reductase ( BV899_18870 ), thioredoxin reductase ( BV899_02360 ), peroxiredoxin ( BV899_10955 ), and superoxide dismutase ( BV899_06125 ), was assessed using real-time PCR ( Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

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Selenite Reduction and the Biogenesis of Selenium Nanoparticles by Alcaligenes faecalis Se03 Isolated from the Gut of Monochamus alternatus (Coleoptera: Cerambycidae)

Wang

Shu

Zhou

et al. 2018

IJMS

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In this study, a bacterial strain exhibiting high selenite (Na2SeO3) tolerance and reduction capacity was isolated from the gut of Monochamus alternatus larvae and identified as Alcaligenes faecalis Se03. The isolate exhibited extreme tolerance to selenite (up to 120 mM) when grown aerobically. In the liquid culture medium, it was capable of reducing nearly 100% of 1.0 and 5.0 mM Na2SeO3 within 24 and 42 h, respectively, leading to the formation of selenium nanoparticles (SeNPs). Electron microscopy and energy dispersive X-ray analysis demonstrated that A. faecalis Se03 produced spherical electron-dense SeNPs with an average hydrodynamic diameter of 273.8 ± 16.9 nm, localized mainly in the extracellular space. In vitro selenite reduction activity and real-time PCR indicated that proteins such as sulfite reductase and thioredoxin reductase present in the cytoplasm were likely to be involved in selenite reduction and the SeNPs synthesis process in the presence of NADPH or NADH as electron donors. Finally, using Fourier-transform infrared spectrometry, protein and lipid residues were detected on the surface of the biogenic SeNPs. Based on these observations, A. faecalis Se03 has the potential to be an eco-friendly candidate for the bioremediation of selenium-contaminated soil/water and a bacterial catalyst for the biogenesis of SeNPs.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Selenite Reduction and the Biogenesis of Selenium Nanoparticles by Alcaligenes faecalis Se03 Isolated from the Gut of Monochamus alternatus (Coleoptera: Cerambycidae)

Wang

Shu

Zhou

et al. 2018

IJMS

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“…Recently, a chromate reductase CsrF in Alishewanella sp. was shown to be essential for Se (IV) reduction to generate BioSeNPs in vivo and in vitro under aerobic condition 45 . In C. testosteroni S44, trxB encoding an enzyme catalyzing the transformation of selenite to selenopersulfide, selD encoding a selenophosphate synthetase and selA encoding a selenocysteine synthase were found on the genome.…”

Section: Discussionmentioning

confidence: 99%

Proteins enriched in charged amino acids control the formation and stabilization of selenium nanoparticles in Comamonas testosteroni S44

Yang

Wang

et al. 2018

Sci Rep

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Elemental selenium nanoparticles (SeNPs) are useful in medicine, environmental remediation and in material science. Biosynthesized SeNPs (BioSeNPs) by bacteria are cheap, eco-friendly and have a lower cytotoxicity in comparison with chemically synthesized ones. Organic matters were found to cap on the surface of BioSeNPs, but the functions were still not entirely clear. The purified BioSeNPs were coated in a thick layer of organic substrates observed by transmission electron microscopy (TEM). Fourier Transform Infrared (FT-IR) and quantitative detection of the coating agents showed that one gram of purified BioSeNPs bound 1069 mg proteins, 23 mg carbohydrates and only very limited amounts of lipids. Proteomics of BioSeNPs showed more than 800 proteins bound to BioSeNPs. Proteins enriched in charged amino acids are the major factor thought to govern the formation process and stabilization of BioSeNPs in bacteria. In view of the results reported here, a schematic model for the molecular mechanism of BioSeNPs formation in bacteria is proposed. These findings are helpful for the artificial green synthesis of stable SeNPs under specific condition and guiding the surface modification of SeNPs for medicine application.

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“…CKCr-6A (Mesbahi-Nowrouzi & Mollania, 2018), Alishewanella sp. WH16-1 (Xia et al, 2018), Васillus subtilis IMV B-7392 (Tymoshok et al, 2019) were used.…”

Section: Eco-friendly Synthesis Of Nanoparticles By Bacteriamentioning

confidence: 99%

Bacterial synthesis of nanoparticles: A green approach

et al. 2020

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In recent decades, the attention of scientists has been drawn towards nanoparticles (NPs) of metals and metalloids. Traditional methods for the manufacturing of NPs are now being extensively studied. However, disadvantages such as the use of toxic agents and high energy consumption associated with chemical and physical processes impede their continued use in various fields. In this article, we analyse the relevance of the use of living systems and their components for the development of "green" synthesis of nano-objects with exceptional properties and a wide range of applications. The use of nano-biotechnological methods for the synthesis of nanoparticles has the potential of large-scale application and high commercial potential. Bacteria are an extremely convenient target for green nanoparticle synthesis due to their variety and ability to adapt to different environmental conditions. Synthesis of nanoparticles by microorganisms can occur both intracellularly and extracellularly. It is known that individual bacteria are able to bind and concentrate dissolved metal ions and metalloids, thereby detoxifying their environment. There are various bacteria cellular components such as enzymes, proteins, peptides, pigments, which are involved in the formation of nanoparticles. Bio-intensive manufacturing of NPs is environmentally friendly and inexpensive and requires low energy consumption. Some biosynthetic NPs are used as heterogeneous catalysts for environmental restoration, exhibiting higher catalytic efficiency due to their stability and increased biocompatibility. Bacteria used as nanofactories can provide a new approach to the removal of metal or metalloid ions and the production of materials with unique properties. Although a wide range of NPs have been biosynthetic and their synthetic mechanisms have been proposed, some of these mechanisms are not known in detail. This review focuses on the synthesis and catalytic applications of NPs obtained using bacteria. Known mechanisms of bioreduction and prospects for the development of NPs for catalytic applications are discussed.

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Novel bacterial selenite reductase CsrF responsible for Se(IV) and Cr(VI) reduction that produces nanoparticles in Alishewanella sp. WH16-1

Cited by 95 publications

References 47 publications

Selenite Reduction and the Biogenesis of Selenium Nanoparticles by Alcaligenes faecalis Se03 Isolated from the Gut of Monochamus alternatus (Coleoptera: Cerambycidae)

Selenite Reduction and the Biogenesis of Selenium Nanoparticles by Alcaligenes faecalis Se03 Isolated from the Gut of Monochamus alternatus (Coleoptera: Cerambycidae)

Proteins enriched in charged amino acids control the formation and stabilization of selenium nanoparticles in Comamonas testosteroni S44

Bacterial synthesis of nanoparticles: A green approach

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