Novel Asymmetric Wettable AgNPs/Chitosan Wound Dressing: In Vitro and In Vivo Evaluation

Abstract

A novel silver nanoparticles (AgNPs)/chitosan composite dressing with asymmetric wettability surfaces was successfully prepared via a simple two-step method for biomedical applications as wound healing materials. First, AgNPs were assembled into the chitosan sponge which was prepared by lyophilization process. Then one side of the sponge was modified by a thin layer of stearic acid. The incorporation of AgNPs into chitosan dressing could enhance the antibacterial activity against drug-sensitive and drug-resistant pathogenic bacteria. The asymmetric surface modification endows the dressing with both highly hydrophobic property and inherent hydrophilic nature of chitosan. The hydrophobic surface of the dressing shows waterproof and antiadhesion for contaminant properties, whereas the hydrophilic surface preserves its water-absorbing capability and efficiently inhibits the growth of bacteria. Furthermore, the AgNPs/chitosan composite dressing displays improved moisture retention and blood clotting ability compared to the unmodified dressings. Cytocompatibility test evaluated in vitro and in a wound infection model illustrates the nontoxic nature of the composite dressing. More importantly, the in vivo wound healing model evaluation in mice reveals that the asymmetric AgNPs/chitosan dressing promotes the wound healing and accelerates the reepithelialization and collagen deposition. The silver accumulation in mice body treated by the composite dressing is far lower than that of the clinically used Acasin nanosilver dressing treated mice. This work indicates the huge potential of the novel AgNPs/chitosan wound dressing with asymmetrical wettability for clinical use.

Nanosilver coated Chitin derived membrane as an Eco-friendly Dressing and Its Effect on Wound Healing

“…There was no difference in the length of neonatal epithelium in each group after 3 days of injury. This was mainly due to the fact that the edge of the wound was still mainly in the preparation period of cell proliferation and migration, and the neonatal epithelium was not obvious [18]. there were no length difference among groups after 3 days of injury (P > 0.05), while the wound epithelial lengths of blank, A, B, C, D groups after 7 days injury were respectively 618.5 μm, 703.7 μm, 810.2 μm, 879.4 μm and 951.0 μm, namely, D > C > B > A > Control(P<0.05).…”

Ultrasmall Gold Nanocluster Based Antibacterial Nanoaggregates for Infectious Wound Healing

“…Typically, they offer a greater number of functional sites compared to commercially available drugs/antibiotics which enhance the interaction with a given microbe. In the past few years, several antimicrobial nanomaterials have been developed which are proven to be highly effective against various multi‐drug resistant bacteria triggered wound infection . The efficacy of these nanomaterials can be further improved by reducing their size .…”

An asymmetric wettable chitosan–silk fibroin composite dressing with fixed silver nanoparticles for infected wound repair: in vitro and in vivo evaluation

“…The outside of the dressing (the side exposed to the air) should exhibit hydrophobicity, which can enable the dressing to act as a barrier against outside risk factors. [17][18][19] Although some wound dressings, such as Syvek-Patch, Chitopack C, Tegasorb, HemCon Bandage, and KytoCel, have been applied in clinical practice and are commercially available, 15,[20][21][22][23] their treatment outcomes are far from satisfactory. As a type of articial antibacterial material, silver nanoparticles (AgNP) have a small particle size, large surface area, and broadspectrum bactericidal effects without causing resistant bacteria.…”