Novel arsenic hyper-resistant bacteria from an extreme environment, Crven Dol mine, Allchar, North Macedonia

Bermanec, Vladimir; Paradžik, Tina; Kazazić, Snježana; Venter, Chantelle; Hrenović, Jasna; Vujaklija, Dušica; Duran, Robert; Boev, Ivan; Boev, Blažo

doi:10.1016/j.jhazmat.2020.123437

Cited by 19 publications

(6 citation statements)

References 39 publications

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“…Additionally, Stenotrophomonas spp . was reported as a novel arsenic hyper-resistant bacteria with an MIC of > 32 mM for arsenite isolated from Crven Dol mine [ 49 ]. However, in this investigation, autotrophic GW isolates were found to have more As (III) resistance than heterotrophic isolates.…”

Section: Discussionmentioning

confidence: 99%

Metagenomic and culture-dependent approaches unveil active microbial community and novel functional genes involved in arsenic mobilization and detoxification in groundwater

Diba,

Hoque,

Rahman

et al. 2023

BMC Microbiol

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Background Arsenic (As) and its species are major pollutants in ecological bodied including groundwater in Bangladesh rendering serious public health concern. Bacteria with arsenotrophic genes have been found in the aquifer, converting toxic arsenite [As (III)] to less toxic arsenate [As (V)] that is easily removed using chemical and biological trappers. In this study, genomic and metagenomic approaches parallel to culture-based assay (Graphical abstract) have made it possible to decipher phylogenetic diversity of groundwater arsenotrophic microbiomes along with elucidation of their genetic determinants. Results Seventy-two isolates were retrieved from six As-contaminated (average As concentration of 0.23 mg/L) groundwater samples from Munshiganj and Chandpur districts of Bangladesh. Twenty-three isolates harbored arsenite efflux pump (arsB) gene with high abundance, and ten isolates possessing arsenite oxidase (aioA) gene, with a wide range of minimum inhibitory concentration, MICAs (2 to 32 mM), confirming their role in arsenite metabolism. There was considerable heterogeneity in species richness and microbial community structure. Microbial taxa from Proteobacteria, Firmicutes and Acidobacteria dominated these diversities. Through these combinatorial approaches, we have identified potential candidates such as, Pseudomonas, Acinetobacter, Stenotrophomonas, Achromobacter, Paraburkholderia, Comamonas and Klebsiella and associated functional genes (arsB, acr3, arsD, arsH, arsR) that could significantly contribute to arsenite detoxification, accumulation, and immobilization. Conclusions Culture-dependent and -independent shotgun metagenomic investigation elucidated arsenotrophic microbiomes and their functions in As biogeochemical transformation. These findings laid a foundation for further large-scale researches on the arsenotrophic microbiomes and their concurrent functions in As biogeochemical transformation in As-contaminated areas of Bangladesh and beyond.

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Section: Discussionmentioning

confidence: 99%

Metagenomic and culture-dependent approaches unveil active microbial community and novel functional genes involved in arsenic mobilization and detoxification in groundwater

Diba,

Hoque,

Rahman

et al. 2023

BMC Microbiol

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“…and Microbacterium spp. contain diverse As-efflux systems enabling a notably high As resistance (0.564 M As 5+ and 0.209 M As 3+ ) ( Bermanec et al, 2021 ).…”

Section: Heavy Metal Resistance Mechanismsmentioning

confidence: 99%

Synthetic bacteria for the detection and bioremediation of heavy metals

Thai

Lim

2023

Front. Bioeng. Biotechnol.

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Toxic heavy metal accumulation is one of anthropogenic environmental pollutions, which poses risks to human health and ecological systems. Conventional heavy metal remediation approaches rely on expensive chemical and physical processes leading to the formation and release of other toxic waste products. Instead, microbial bioremediation has gained interest as a promising and cost-effective alternative to conventional methods, but the genetic complexity of microorganisms and the lack of appropriate genetic engineering technologies have impeded the development of bioremediating microorganisms. Recently, the emerging synthetic biology opened a new avenue for microbial bioremediation research and development by addressing the challenges and providing novel tools for constructing bacteria with enhanced capabilities: rapid detection and degradation of heavy metals while enhanced tolerance to toxic heavy metals. Moreover, synthetic biology also offers new technologies to meet biosafety regulations since genetically modified microorganisms may disrupt natural ecosystems. In this review, we introduce the use of microorganisms developed based on synthetic biology technologies for the detection and detoxification of heavy metals. Additionally, this review explores the technical strategies developed to overcome the biosafety requirements associated with the use of genetically modified microorganisms.

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“…Microbacterium spp. isolated from Creven Dol Mine, Allchar, North Macedonia-survived in presence of 209 mM arsenite and 564 mM arsenate [42].…”

Section: S Rdna Sequencing and Phylogenetic Analysis Identified The B...mentioning

confidence: 99%

Isolation and Identification of Arsenic Hyper-Tolerant and Plant Growth Promoting Bacterium from Soil

Mandal¹,

Aghababaei²,

Das³

et al. 2022

Preprint

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The soil and groundwater of Bhagobangola I block of Murshidabad district, West Bengal, India is severely arsenic contaminated. A bacterium was isolated from the garden soil of Mahishasthali village, which could tolerate 36.49 mM arsenic (III), 280.44 mM arsenic (V) and 63 mM chromium (III), which makes it arsenic (III & V) and chromium (III) hyper-tolerant bacterium. The growth pattern of this bacterium does not show much alteration in presence of 10 mM arsenic (III) and chromium (III), emphasizing its resistance to these heavy metals. Scanning electron microscopy depicted the size of this bacterium to be ~1.45 µm. 16S rDNA sequencing followed by subsequent phylogenetic analysis established the identity of this bacterium as Microbacterium paraoxydans. The bacterium is capable of bioremediation of arsenic and showed 30.8% & 22.6%, and 35.2% and 30.5% of bioremediation over a period of 24 and 48 hours in 1 mM and 4 mM arsenite, respectively. Microbacterium paraoxydans also exhibit plant growth promoting properties like nitrogen fixation, phosphate solubilization, indole-3-acetic acid production and production of siderophores. Therefore, the heavy metal resistance, bioremediation potential and plant growth promoting potential of the bacterium could be utilized not only for reduction of arsenic toxicity in soil and groundwater but also for plant growth promotion by using it as a biofertilizer.

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Novel arsenic hyper-resistant bacteria from an extreme environment, Crven Dol mine, Allchar, North Macedonia

Cited by 19 publications

References 39 publications

Metagenomic and culture-dependent approaches unveil active microbial community and novel functional genes involved in arsenic mobilization and detoxification in groundwater

Metagenomic and culture-dependent approaches unveil active microbial community and novel functional genes involved in arsenic mobilization and detoxification in groundwater

Synthetic bacteria for the detection and bioremediation of heavy metals

Isolation and Identification of Arsenic Hyper-Tolerant and Plant Growth Promoting Bacterium from Soil

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