Novel aromatic polyketides from soil Streptomyces spp.: purification, characterization and bioactivity studies

Bundale, Sunita; Begde, Deovrat; Pillai, Dhanashree; Gangwani, Karishma; Nashikkar, Nandita; Kadam, Tukaram; Upadhyay, Avinash

doi:10.1007/s11274-018-2448-1

Cited by 10 publications

(4 citation statements)

References 34 publications

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“…However, the shape of UV-Vis spectrum can present variation with shift of λ max value according to the pigment concentration, sugar present in solution (result not shown) or solvent used (see below). Against reported studies attributing antibacterial activity to anthracyclines [24,25] especially prodigiosin [26], antibacterial assay of the studied crud extract pigments was negative on both gram negative and positive test bacteria (Figure 4).…”

Section: Color Antibacterial Spectral and Tlc Analysis Of Pigmentsmentioning

confidence: 80%

Physico-chemical Characterization of a Pink Red-like Pigments Produced by Five New Bacterial Soil Strains Identified as <i>Streptomyces coelicoflavus</i>

Assia¹,

Hasnaa²,

Sara³

et al. 2018

AJMR

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Five new strains MFB11, MFB20, MFB21, MFB23 and MFB24 of actinomycetes showed an intracellular hydrophobic pink red-like pigment production. These pigments present similar physico-chemical characteristics with anthracycline antibiotics of prodigiosin family. Nevertheless, negative antibacterial assay, Thinlayer chromatography (TLC) and interaction with organic solvents analysis of these pigments revealed their difference from known anthracycline antibiotics. Morphological, biochemical and gene coding 16S RNA sequence analysis allowed identification of the producer strains as Streptomyces coelicoflavus; known to produce important aminoglycoside antibiotics and other bioactive compounds but not anthracyclines red-like pigments. The identification of the five strains and physico-chemical properties of the produced pink red-like pigments are presented in this report.

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Section: Color Antibacterial Spectral and Tlc Analysis Of Pigmentsmentioning

confidence: 80%

Physico-chemical Characterization of a Pink Red-like Pigments Produced by Five New Bacterial Soil Strains Identified as <i>Streptomyces coelicoflavus</i>

Assia¹,

Hasnaa²,

Sara³

et al. 2018

AJMR

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“…Recently, by optimizing promoters, enzymes, and chassis cells, a recombinant Streptomyces strain was engineered, which produced 15–20 mg/L aklavinone [ 131 ]. More importantly, a total of 37 genomes were shown to harbor the gene clusters for the biosynthesis of aclacinomycin, indicating the feasibility of screening strains for the high-level production of ACM-A [ 132 , 133 ].…”

Section: Strategies For Improving Acm-a Productionmentioning

confidence: 99%

Rethinking Biosynthesis of Aclacinomycin A

Tian

2023

Molecules

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Aclacinomycin A (ACM-A) is an anthracycline antitumor agent widely used in clinical practice. The current industrial production of ACM-A relies primarily on chemical synthesis and microbial fermentation. However, chemical synthesis involves multiple reactions which give rise to high production costs and environmental pollution. Microbial fermentation is a sustainable strategy, yet the current fermentation yield is too low to satisfy market demand. Hence, strain improvement is highly desirable, and tremendous endeavors have been made to decipher biosynthesis pathways and modify key enzymes. In this review, we comprehensively describe the reported biosynthesis pathways, key enzymes, and, especially, catalytic mechanisms. In addition, we come up with strategies to uncover unknown enzymes and improve the activities of rate-limiting enzymes. Overall, this review aims to provide valuable insights for complete biosynthesis of ACM-A.

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“…Bands were visualized under ultraviolet light. Retention factors (Rf) of the bands were then calculated (Bundale et al, 2018).…”

Section: Thin Layer Chromatographymentioning

confidence: 99%

Tromethamine and Dodecanol-Like Compounds Appear to be the Major Secondary Metabolites of Streptomyces Decoyicus (M*)

Çelik

Aksu

Yenidünya

et al. 2022

Preprint

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An isolate of Streptomyces decoyicus was identified by the sequencing of 16S rRNA gene. It was grown on solid media (250 ml) and secondary metabolites were extracted with n-butanol (100 ml). The extract was dried and run in a sodium dodecyl sulphate-polyacrylamide gel (SDS-PAGE, 10%). Two main bands obtained were sliced and the metabolites were regained in n-butanol (100µl). These two samples were then identified by gas-chromatography-mass spectrometry (GC-MS), and Fourier-transform infrared spectroscopy (FT-IR). The results demonstrated that tromethamine- and 1-dodecanol were the main constituents (band 1: 61% and 17.7%; band 2: 41% and 54%, respectively). This finding maintained that the isolate of Streptomyces decoyicus produced high amounts tromethamine- and 1-dodecanol-like compounds under the conditions investigated.

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Novel aromatic polyketides from soil Streptomyces spp.: purification, characterization and bioactivity studies

Cited by 10 publications

References 34 publications

Physico-chemical Characterization of a Pink Red-like Pigments Produced by Five New Bacterial Soil Strains Identified as <i>Streptomyces coelicoflavus</i>

Physico-chemical Characterization of a Pink Red-like Pigments Produced by Five New Bacterial Soil Strains Identified as <i>Streptomyces coelicoflavus</i>

Rethinking Biosynthesis of Aclacinomycin A

Tromethamine and Dodecanol-Like Compounds Appear to be the Major Secondary Metabolites of Streptomyces Decoyicus (M*)

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