Novel aqueous two-phase systems composed of acetonitrile and polyols: Phase diagrams and extractive performance

Cardoso, Gustavo de Brito; Souza, Isabela Nascimento; Mourão, Teresa; Freire, Mara G.; Soares, Cleide Mara Faria; Lima, Álvaro Silva

doi:10.1016/j.seppur.2014.01.004

Cited by 59 publications

(13 citation statements)

References 45 publications

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“…Since the bulk of the two phases consists mainly of water, ABS are environmentally friendly and provide a biocompatible media for biomaterials. ABS have been utilized in the extraction and purification of diverse biomolecules such as proteins, enzymes, nucleic acids, flavor compounds, antioxidants and antibiotics [10,11]. It is interesting to note that polymer-carbohydrate ABS offer greener extraction procedures than polymer-ionic liquid and traditional polymer-salt ABS.…”

Section: Introductionmentioning

confidence: 99%

Soluting-in and soluting-out of water-soluble polymers in aqueous carbohydrate solutions studied by vapor pressure osmometry

Ebrahimi

Sadeghi

2017

Journal of Molecular Liquids

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Precise vapor pressure osmometry measurements at 308.15 K have been performed for ternary systems of carbohydrate in aqueous 0.2 w/w polymer solutions. The polymers are polyethylene glycol 400 (PEG400), polyethylene glycol 10000 (PEG10000) and polypropylene glycol 400 (PPG400), and the carbohydrates are xylose, xylitol, glucose, fructose, sucrose and raffinose. In order to study the soluting-out and soluting-in effects occurring in the polymer-carbohydrate aqueous systems, deviations of vapor-liquid equilibria behavior of these systems from the semiideal state have been evaluated. In the case of PPG + carbohydrates aqueous systems, because of unfavorable PPG-carbohydrate interactions, the solutes exclude themselves from the vicinity of each other and therefore aqueous biphasic systems are entropically formed above a critical concentration. The monophasic and biphasic regions of these systems show, respectively, negative and positive deviations from the semi-ideal behavior, which become more negative and more positive with increasing hydrophilicity of the carbohydrates. However, in the case of PEG + carbohydrate aqueous systems, which are completely miscible because of favorable PEGcarbohydrate interactions, the interaction of each solute with water molecules becomes weaker in the presence of the other solute. These systems show positive deviations from the semi-ideal behavior. By increasing the PEG molar mass and decreasing the hydrophilicity of carbohydrates,

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Section: Introductionmentioning

confidence: 99%

Soluting-in and soluting-out of water-soluble polymers in aqueous carbohydrate solutions studied by vapor pressure osmometry

Ebrahimi

Sadeghi

2017

Journal of Molecular Liquids

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“…Maltitol is a non-ionic compound with an enhanced ability to be hydrated due to its large number of –OH groups, which makes it a strong phase separating agent. Due to its strong phase separating characteristics, maltitol improves the migration of lipase to the opposite phase [ 6 ], which was Triton X-100-rich top phase, hence, increasing the efficiency of the system during lipase partitioning process.…”

Section: Resultsmentioning

confidence: 99%

“…The evaluation of the lipase partitioning experiments was achieved based on the calculations of several parameters. The following parameters were calculated according to Show [ 14 ] and de Brito Cardoso et al [ 6 ]. The partition coefficient of lipase (K) was calculated based on Equation (7):

where A T and A B were lipase activity (U/mL) in top and bottom phases, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, additional laborious purification techniques, such as ultrafiltration, diafiltration and crystallization are necessary for the removal of phase-forming chemicals/polymers from the target protein [ 5 ]. In order to overcome the weaknesses of the traditional ATPS systems, new ATPS systems composed of non-ionic surfactants and polyols have been discovered and explored [ 6 , 7 ]. When non-ionic surfactants are used as a component in ATPS, the system will separate into two phases, in which one of the phases will be the surfactant-depleted phase (water-rich phase) whereas the other phase will be the surfactant-rich phase.…”

Section: Introductionmentioning

confidence: 99%

“…Polyols like maltitol, which are also known as sugar alcohols, are a hydrogenated form of carbohydrates and their carbonyl group has been reduced to a primary or secondary hydroxyl group. They are able to mimic the structure of water; therefore, they are able to form and maintain an artificial sphere of hydration around the target biomolecules [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Purification and Characterization of Lipase Produced by Leuconostoc mesenteroides Subsp. mesenteroides ATCC 8293 Using an Aqueous Two-Phase System (ATPS) Composed of Triton X-100 and Maltitol

et al. 2018

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Purification of lipase produced by L. mesenteroides subsp. mesenteroides ATCC 8293 was conducted for the first time using a novel aqueous two-phase system (ATPS) composed of Triton X-100 and maltitol. The partitioning of lipase was optimized according to several parameters including pH, temperature, and crude load. Results showed that lipase preferentially migrated to the Triton X-100 rich phase and optimum lipase partitioning was achieved in ATPS at TLL of 46.4% and crude load of 20% at 30 °C and pH 8, resulting in high lipase purification factor of 17.28 and yield of 94.7%. The purified lipase showed a prominent band on SDS-PAGE with an estimated molecular weight of 50 kDa. The lipase was stable at the temperature range of 30–60 °C and pH range of 6–11, however, it revealed its optimum activity at the temperature of 37 °C and pH 8. Moreover, lipase exhibited enhanced activity in the presence of non-ionic surfactants with increased activity up to 40%. Furthermore, results exhibited that metals ions such as Na+, Mg2+, K+ and Ca2+ stimulated lipase activity. This study demonstrated that this novel system could be potentially used as an alternative to traditional ATPS for the purification and recovery of enzymes since the purified lipase still possesses good process characteristics after undergoing the purification process.

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Ionic liquid‐based aqueous biphasic systems as a versatile tool for the recovery of antioxidant compounds

Santos

Silva

Ventura

et al. 2014

Biotechnology Progress

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The comparative evaluation of distinct types of ionic liquid-based aqueous biphasic systems (IL-ABS) and more conventional polymer/salt-based ABS to the extraction of two antioxidants, eugenol and propyl gallate, is focused. In a first approach, IL-ABS composed of ILs and potassium citrate (C6H5K3O7/C6H8O7) buffer at pH 7 were applied to the extraction of two antioxidants, enabling the assessment of the impact of IL cation core on the extraction. The second approach uses ABS composed of polyethylene glycol (PEG) and potassium phosphate (K2HPO4/KH2PO4) buffer at pH 7 with imidazolium-based ILs as adjuvants. Their application to the extraction of the compounds allowed the investigation of the impact of the presence/absence of IL, the PEG molecular weight, and the alkyl side chain length of the imidazolium cation on the partition. It is possible to maximize the extractive performance of both antioxidants up to 100% using both types of IL-ABS. The IL enhances the performance of ABS technology. The data puts in evidence the pivotal role of the appropriate selection of the ABS components and design to develop a successful extractive process, from both environmental and performance points of view.

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Novel aqueous two-phase systems composed of acetonitrile and polyols: Phase diagrams and extractive performance

Cited by 59 publications

References 45 publications

Soluting-in and soluting-out of water-soluble polymers in aqueous carbohydrate solutions studied by vapor pressure osmometry

Soluting-in and soluting-out of water-soluble polymers in aqueous carbohydrate solutions studied by vapor pressure osmometry

Purification and Characterization of Lipase Produced by Leuconostoc mesenteroides Subsp. mesenteroides ATCC 8293 Using an Aqueous Two-Phase System (ATPS) Composed of Triton X-100 and Maltitol

Ionic liquid‐based aqueous biphasic systems as a versatile tool for the recovery of antioxidant compounds

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