Novel aptasensor for the ultrasensitive detection of kanamycin based on grapheneoxide quantum-dot-linked single-stranded DNA-binding protein

He, Yunhui; Wen, Xiaoye; Zhang, Bingyan; Fan, Zhefeng

doi:10.1016/j.snb.2018.03.029

Cited by 29 publications

(12 citation statements)

References 43 publications

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“…Upon addition of the target, kanamycin, the quencher-labeled aptamer would dissociate from the GOQDs resulting in increased fluorescence. This method showed a LOD of 6 pg mL À1 kanamycin in milk [52]. On top of that, a biosensor using the luminescence resonance energy transfer (LRET) from UCNPs to SYBR Green I dye was established [99].…”

Section: Sensors Based On Structure-switching Strategiesmentioning

confidence: 99%

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Nanomaterial-based fluorescent biosensor for veterinary drug detection in foods

Tao¹,

Peng²,

Liu³

2020

Journal of Food and Drug Analysis

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Veterinary drugs have been widely used in the food industry. Their residues in food products need to be tightly regulated to ensure food safety. In particular, some veterinary drugs are still used illegally, although they have already been banned. Nanomaterials are playing an increasingly important role in analytical chemistry due to their unique properties. Compared to traditional organic dyes and colloidal gold nanoparticle labels, fluorescent nanomaterials appear particularly attractive for the detection of veterinary drug residues. This review summarizes recent advancements of fluorescent biosensors using nanomaterials for the detection of veterinary drug residues in foods. The useful properties of each type of fluorescent nanomaterial are first discussed such as large Stokes shifts, long emission lifetime, and high quantum yields, which are useful for detection in food-related sample matrix. The following target recognition molecules are then reviewed individually including antibodies, aptamers, molecularly imprinted polymers, and metal ion coordination based ligands. Representative bioconjugation and assay methods are discussed for each recognition mechanism. Finally, a few future research directions are outlined in the last section of this review.

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Section: Sensors Based On Structure-switching Strategiesmentioning

confidence: 99%

“…Schematic illustration of assays based on the structure-switching aptamer strategies for the detection of veterinary drugs. (a) Assays based on FRET (figure created based on ref [52,98]…”

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confidence: 99%

Nanomaterial-based fluorescent biosensor for veterinary drug detection in foods

Tao¹,

Peng²,

Liu³

2020

Journal of Food and Drug Analysis

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“…Thus, the addition of kanamycin caused the aptamer to lose its binding ability to the SSB and led to the subsequent FL recovery. The GQDs-based FL sensing platform showed a linear range of 0.017-154.4 nM for the detection of kanamycin and a LOD of 0.01 nM [37]. Aucélio's group prepared FL GQDs by using citrate and glutathione as the starting materials.…”

Section: Turn-on Fluorescent Analysis Strategiesmentioning

confidence: 99%

“…Representative (A) turn-on (reprinted with permission from[37] Copyright (2018) Elsevier) and (B) ratio FL detection for kanamycin based on fluorescence resonance energy transfer (FRET) (reproduced with permission from[39] Copyright (2019) Royal Society of Chemistry).…”

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confidence: 99%

Recent Advances in Optical Detection of Aminoglycosides

Zhou

Cao

2020

Applied Sciences

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Aminoglycosides (AGs) are broad-spectrum antibiotics used in both human infection and animal medicine. The overuse of AGs causes undesirable residues in food, leading to serious health problems due to food chain accumulation. In recent years, various methods have been developed to determine AGs in food. Among these methods, fluorescent (FL), colorimetric and chemiluminescent (CL) optical methods possess advantages such as their simple instrumentation, low cost, simple operation, feasibility of realizing visualization, and smartphone imaging. This mini-review summarizes optical assays for the detection of AGs in food developed in recent years. The detection principles for different categories are discussed. Then, the amplification techniques for the ultrasensitive detection of AGs are introduced. We also discuss multiplex methods for the simultaneous detection of AGs. Finally, the challenges and future prospects are discussed in the Conclusions and Perspectives section.

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“…To induce target-specific GNP aggregation, GNPs have been functionalized with target-recognizing molecules, such as single-stranded DNA (ssDNA) [ 59 , 60 ], antibodies [ 67 ], and aptamers [ 68 , 69 , 70 , 71 ]. After Mirkin and his coworkers reported a biosensor in 1997 which can analyze a single mismatch in the hybridized DNA by using the ssDNA-conjugated GNPs [ 59 ], DNA and RNA samples have been analyzed with an interparticle crosslinking aggregation-based colorimetry method [ 54 ].…”

Section: Gold Nanoparticle-based Colorimetric Biosensorsmentioning

confidence: 99%

Application of Gold Nanoparticle to Plasmonic Biosensors

Lee

Cho

Choi

et al. 2018

IJMS

126

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Gold nanoparticles (GNPs) have been widely utilized to develop various biosensors for molecular diagnosis, as they can be easily functionalized and exhibit unique optical properties explained by plasmonic effects. These unique optical properties of GNPs allow the expression of an intense color under light that can be tuned by altering their size, shape, composition, and coupling with other plasmonic nanoparticles. Additionally, they can also enhance other optical signals, such as fluorescence and Raman scattering, making them suitable for biosensor development. In this review, we provide a detailed discussion of the currently developed biosensors based on the aforementioned unique optical features of GNPs. Mainly, we focus on four different plasmonic biosensing methods, including localized surface plasmon resonance (LSPR), surface-enhanced Raman spectroscopy (SERS), fluorescence enhancement, and quenching caused by plasmon and colorimetry changes based on the coupling of GNPs. We believe that the topics discussed here are useful and able to provide a guideline in the development of novel GNP-based biosensors in the future.

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Novel aptasensor for the ultrasensitive detection of kanamycin based on grapheneoxide quantum-dot-linked single-stranded DNA-binding protein

Cited by 29 publications

References 43 publications

Nanomaterial-based fluorescent biosensor for veterinary drug detection in foods

Nanomaterial-based fluorescent biosensor for veterinary drug detection in foods

Recent Advances in Optical Detection of Aminoglycosides

Application of Gold Nanoparticle to Plasmonic Biosensors

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