Novel approaches towards a detailed control of the mechanism and efficiency of photosensitized processes in vivo

Jori, Giulio; Schindl, Liesbeth; Schindl, Andreas; Polo, Luz

doi:10.1016/s1010-6030(96)04371-7

Cited by 43 publications

(31 citation statements)

References 20 publications

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“…[7][8][9] The laser light emitting at the visible red spectrum stimulates the respiratory chain in cells under stress, leading to alterations in electron transport by mitochondria. 16,17,19,22,25 Then, besides the increase in ATP synthesis, there is signaling to the nucleus stimulating the production of antioxidant enzymes, proteins that stabilize cellular function and proliferation. 20,21,16,17,22 On the other hand, irradiation in the infrared spectrum stimulates calcium channels of the cell membrane.…”

Section: Discussionmentioning

confidence: 99%

“…16,17,19,22,25 Then, besides the increase in ATP synthesis, there is signaling to the nucleus stimulating the production of antioxidant enzymes, proteins that stabilize cellular function and proliferation. 20,21,16,17,22 On the other hand, irradiation in the infrared spectrum stimulates calcium channels of the cell membrane. 18 The increase in intracellular Ca 2+ gradient may explain the increased cell viability observed in the treated group compared to control subjected to the same stress conditions, where this stimulation facilitates the occurrence of cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

“…[12][13][14] The laser light within the red visible and near infrared wavelengths corresponds to the energy absorption spectrum of the respiratory chain components, increasing the cellular metabolism under stress conditions. 12,[15][16][17][18] The photon energy is converted into chemical energy within the cell, forming ATP, 5,16 which may lead to increased intracellular Ca +2 . 18,19 This will ultimately stimulate duplication of DNA, 20,21 protein synthesis increase, 13 induction of the action of enzymes that control the oxidative stress, 12,17,22 modulation of fibroblast growth factors production, which in turn will stimulate cell proliferation.…”

Section: Introductionmentioning

confidence: 99%

“…12,[15][16][17][18] The photon energy is converted into chemical energy within the cell, forming ATP, 5,16 which may lead to increased intracellular Ca +2 . 18,19 This will ultimately stimulate duplication of DNA, 20,21 protein synthesis increase, 13 induction of the action of enzymes that control the oxidative stress, 12,17,22 modulation of fibroblast growth factors production, which in turn will stimulate cell proliferation. 15,17,21,[23][24][25] Based on the above, phototherapy with low intensity laser would be of importance for restoring the integrity of dental pulp after bleaching, especially for postoperative symptoms control.…”

Section: Introductionmentioning

confidence: 99%

“…18,19 This will ultimately stimulate duplication of DNA, 20,21 protein synthesis increase, 13 induction of the action of enzymes that control the oxidative stress, 12,17,22 modulation of fibroblast growth factors production, which in turn will stimulate cell proliferation. 15,17,21,[23][24][25] Based on the above, phototherapy with low intensity laser would be of importance for restoring the integrity of dental pulp after bleaching, especially for postoperative symptoms control. Thus, phototherapy with low intensity laser could be used as additional clinical procedure for tooth bleaching.…”

Section: Introductionmentioning

confidence: 99%

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In vitro effect of low intensity laser on the cytotoxicity produced by substances released by bleaching gel

et al. 2010

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This in vitro study aimed to analyze the effect of different parameters of phototherapy with low intensity laser on the viability of human dental pulp fibroblasts under the effect of substances released by bleaching gel. Cells were seeded into 96 wells plates (1 x 10 3 cells/well) and placed in contact with culture medium conditioned by a 35% hydrogen peroxide bleaching gel for 40 minutes, simulating the clinical condition of the in-office bleaching treatment. Cells cultured in ideal growth conditions served as positive control group (PC), and the cells grown in conditioned medium and non-irradiated served as negative control group (NC). Cells grown in conditioned medium were submitted to a single irradiation with a diode laser (40 mW, 0.04 cm²) emitting at visible red (660 nm; RL) or near infrared (780 nm; NIR) using punctual technique, in contact mode and energy densities of 4, 6 or 10 J/cm². The cell viability was analyzed through the MTT reduction assay immediately and 24 hours after the irradiation. The data was compared by ANOVA followed by the Tukey's test (p ≤ 0.05). The cell viability increased significantly in 24 hours within each group. The PC presented cell viability significantly higher than NC in both experimental times. Only the NIR/10 J/ cm² group presented cell viability similar to that of PC in 24 hours. The phototherapy with low intensity laser in defined parameters is able to compensate the cytotoxic effects of substances released by 35% hydrogen peroxide bleaching gel.

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