Novel approaches to study coronary vasculature development in mice

Kolesová, Hana; Bartoš, Martin; Hsieh, Wan Chin; Olejníčková, Veronika; Sedmera, David

doi:10.1002/dvdy.24637

Cited by 21 publications

(18 citation statements)

References 39 publications

(76 reference statements)

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“…Although there are limited studies on the coronary anatomy of murine hearts, which is slightly different from that of humans’, it has been shown that C57BL/6J mice have three main coronaries and that their coronary arteries are intramyocardial rather than epicardial as in humans. In these mice, the left, right and septal coronary arteries (the septal artery may originate from either the left or right coronary, or two septal coronaries may coexist from either 33 ) supply the left and right ventricles, and interventricular septum, respectively 33 – 36 . Thus, SAC-induced hypertensive heart disease predominantly involves collagen deposition, due to fibroblast proliferation 32 , around the arteries branching from the left coronary artery into the LV free wall 34 , 36 rather than from those located in the interventricular septum (IVS), perhaps indicating greater wall stress due to a higher transmural pressure in the LV free wall than that across the IVS.…”

Section: Discussionmentioning

confidence: 99%

Pressure overload by suprarenal aortic constriction in mice leads to left ventricular hypertrophy without c-Kit expression in cardiomyocytes

Nicks

Kesteven

et al. 2020

Sci Rep

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Animal models of pressure overload are valuable for understanding hypertensive heart disease. We characterised a surgical model of pressure overload-induced hypertrophy in C57BL/6J mice produced by suprarenal aortic constriction (SAC). Compared to sham controls, at one week post-SAC systolic blood pressure was significantly elevated and left ventricular (LV) hypertrophy was evident by a 50% increase in the LV weight-to-tibia length ratio due to cardiomyocyte hypertrophy. As a result, LV end-diastolic wall thickness-to-chamber radius (h/R) ratio increased, consistent with the development of concentric hypertrophy. LV wall thickening was not sufficient to normalise LV wall stress, which also increased, resulting in LV systolic dysfunction with reductions in ejection fraction and fractional shortening, but no evidence of heart failure. Pathological LV remodelling was evident by the re-expression of fetal genes and coronary artery perivascular fibrosis, with ischaemia indicated by enhanced cardiomyocyte Hif1a expression. The expression of stem cell factor receptor, c-Kit, was low basally in cardiomyocytes and did not change following the development of robust hypertrophy, suggesting there is no role for cardiomyocyte c-Kit signalling in pathological LV remodelling following pressure overload.

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Section: Discussionmentioning

confidence: 99%

Pressure overload by suprarenal aortic constriction in mice leads to left ventricular hypertrophy without c-Kit expression in cardiomyocytes

Nicks

Kesteven

et al. 2020

Sci Rep

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“…Hearts perfused with polymerized yellow Microfil (Flow Tech) were prepared as described previously (69) and processed by micro-CT scanning (SkyScan 1272; Bruker). Projection images were reconstructed using NRecon SW (Bruker).…”

Section: Methodsmentioning

confidence: 99%

HIF-1α is required for development of the sympathetic nervous system

Bohuslavová

Čerychová

Papoušek

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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The molecular mechanisms regulating sympathetic innervation of the heart during embryogenesis and its importance for cardiac development and function remain to be fully elucidated. We generated mice in which conditional knockout (CKO) of the Hif1a gene encoding the transcription factor hypoxia-inducible factor 1α (HIF-1α) is mediated by an Islet1-Cre transgene expressed in the cardiac outflow tract, right ventricle and atrium, pharyngeal mesoderm, peripheral neurons, and hindlimbs. These Hif1aCKO mice demonstrate significantly decreased perinatal survival and impaired left ventricular function. The absence of HIF-1α impaired the survival and proliferation of preganglionic and postganglionic neurons of the sympathetic system, respectively. These defects resulted in hypoplasia of the sympathetic ganglion chain and decreased sympathetic innervation of the Hif1aCKO heart, which was associated with decreased cardiac contractility. The number of chromaffin cells in the adrenal medulla was also decreased, indicating a broad dependence on HIF-1α for development of the sympathetic nervous system.

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“…Notably, this group also performed TEM analysis, which revealed that BABB‐treated heart integrity is severely compromised, when compared with much less extensive deformation observed in CUBIC‐treated samples . Transparency achievable in Sca l e treated samples, although too low for imaging of adult hearts, is sufficient to study murine hearts at early stages of the embryonic development. As such, application of Sca l e by Li et al .…”

Section: Application Of Toc For Particular Peripheral Organsmentioning

confidence: 99%

Advances in Ex Situ Tissue Optical Clearing

Matryba

Kaczmarek

Gołąb

2019

Laser & Photonics Reviews

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Examination of whole organs with subcellular resolution in health, disease, and during development is necessary to decipher their biological complexity. However, until recently, this has been virtually impossible due to the natural opacity of organ tissue. Recent progress in tissue optical clearing (TOC) has overcome this limitation by turning organs into transparent, light-permitting specimens. At least 20 original TOC methods have been developed in less than a decade, which were followed by hundreds of attempts that were aimed at their optimization and practical application. The majority of proof-of-concept studies have focused on the brain. However, it is apparent that TOC might be equally valuable when applied to peripheral organs or even the whole body. The progress in TOC for peripheral organs is delineated in an organ-by-organ fashion and whole-body clearing approaches are discussed. Additionally, physical and optical approaches for TOC affecting the optical properties of the samples and image quality are discussed to explore their advantages, limitations, and future possibilities.

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Novel approaches to study coronary vasculature development in mice

Cited by 21 publications

References 39 publications

Pressure overload by suprarenal aortic constriction in mice leads to left ventricular hypertrophy without c-Kit expression in cardiomyocytes

Pressure overload by suprarenal aortic constriction in mice leads to left ventricular hypertrophy without c-Kit expression in cardiomyocytes

HIF-1α is required for development of the sympathetic nervous system

Advances in Ex Situ Tissue Optical Clearing

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