Novel approaches for the design, delivery and administration of vaccine technologies

Wallis, Jamie; Shenton, Daniel P.; Carlisle, Robert

doi:10.1111/cei.13287

Cited by 94 publications

(83 citation statements)

References 186 publications

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“…Due to safety issues of heat killed or whole cell antigen vaccines, production of plant‐based vaccines has become a striking strategy for the development of cost‐effective vaccines due to some important features such as: high scalability, low health risks, minimum need of cooling chain, and low cost of downstream processing . Currently, delivery of vaccines via plant systems has gained a high consideration platform for oral administration and its renowned potential to stimulate immunity in human and animals .…”

Section: Introductionmentioning

confidence: 99%

“…The idea of edible vaccine refers to oral administration of antigens expressed in recombinant plants to stimulate the systematic and mucosal immunity against different pathogenic, bacterial, and viral diseases. Upon delivery of vaccine antigen expressed in plants, the cell wall of plants may keep the intracellular substantial and protect them from acidic environment of stomach and within the gastrointestinal tract, these plants would be able to interact with gut‐associated lymphoid tissue (GALT) to stimulate the immune system . These emerging bioencapsulated vaccine antigens expressed in plants provide a cost‐effective platform to deliver different therapeutic proteins in living organisms.…”

Section: Introductionmentioning

confidence: 99%

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Expression of ESAT‐6 antigen from Mycobacterium tuberculosis in broccoli: An edible plant

Saba

Sameeullah

Asghar

et al. 2020

Biotech and App Biochem

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Tuberculosis (TB) is one of the major infectious diseases caused by Mycobacterium tuberculosis. The development of an effective and economical vaccine for controlling TB is essential especially for developing countries. Edible plants can serve as biofactories to produce vaccine antigens. In this study, 6 kDa early secretory antigenic target (ESAT‐6) of M. tuberculosis was expressed in Brassica oleracea var. italica via Agrobacterium‐mediated transformation to facilitate oral delivery of antigen. ESAT‐6 gene was cloned using Gateway® cloning strategy. Transformation and presence of transgene was confirmed through PCR. Expression level of transgene was calculated via quantitative real‐time PCR (qRT‐PCR) and the maximum integrated transgene number was two. Maximum amount of total soluble fraction of ESAT‐6 was evaluated by immunoblotting, estimated to accumulate up to 0.5% of total soluble protein. The recombinant ESAT‐6 protein was further purified and detected using silver staining and Western blotting. ESAT‐6 protein induced humoral immune response in mice immunized orally and subcutaneously. The expression of M. tuberculosis antigen in edible plants could aid in the development of cost‐effective and oral delivery of an antigen‐based subunit vaccine against TB. To the best our knowledge, it is the first report of expression of a vaccine antigen in broccoli.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression of ESAT‐6 antigen from Mycobacterium tuberculosis in broccoli: An edible plant

Saba

Sameeullah

Asghar

et al. 2020

Biotech and App Biochem

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“…Vaccination is one of the main strategies to prevent and control infectious diseases [1]. However, during recent decades, many efforts have been being made to obtain alternatives to conventional intramuscular (IM) or subcutaneous (SC) vaccine administration routes, which are mainly administered via hypodermic needles and need trained healthcare personnel [2]. The intradermal (ID) immunization route is of great interest, due to the easy accessibility to the large surface area of the skin and because it has unique immunological properties.…”

Section: Introductionmentioning

confidence: 99%

Dissolving Microneedles for Intradermal Vaccination against Shigellosis

et al. 2019

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Intradermal (ID) immunization is of increasing interest due to the easy accessibility and excellent immunogenic properties of the skin. Among ID immunization methods, dissolving microneedles (MNs) have appeared as an alternative to traditional hypodermic immunization, offering many advantages, such as being an easily administered method, with no need for health personnel, painless, and avoiding the use of needles and sharp wastage. In this study, an affordable and easy-to-produce MNs method was developed based on aqueous blends of 30% w/w poly (methyl vinyl ether-co-maleic anhydride). As an antigen model, a subunit vaccine candidate based on outer membrane vesicles from Shigella flexneri was used. Both unloaded and antigen-loaded MNs were synthetized and characterized. The MNs were successfully validated in an in vitro Parafilm M ® skin model and in a pig skin ex vivo model. Biodistribution studies were performed in BALB/c mice using 99m TcO 4 − radiolabeled samples. Results indicated that the vesicle vaccine was successfully released from the MNs and targeted gastrointestinal tract after 6 h post-administration. In vivo immunization and protection studies were performed in BALB/c mice. Mice were intradermally immunized through ear skin with one single dose of 200 µg antigenic complex, eliciting the production of specific systemic IgG and mucosal IgA. Moreover, MNs were able to protect mice from an experimental infection with 1×10 6 CFU/mouse of S. flexneri four weeks after immunization. This work demonstrates for the first time the potential of outer membrane vesicle-loaded dissolving MNs for ID vaccination against enteropathogens like Shigella.Vaccines 2019, 7, 159 2 of 15 (SALT), formed by a great network of immune cells, including Langerhans cells (LC), lymphocytes, and dendritic cells (DCs), which recognize the antigens and present them in the proximal lymph nodes [3]. Different ID vaccination methods have been used already, such as the Mantoux technique, microinjection systems, or jet injectors, used for smallpox, rabies, or Bacillus Calmette-Guérin (BCG) vaccines. However, they require the use of needles [4]. Microneedle patches (MNs) have appeared as a novel and attractive approach for ID immunization [5]. They are painless devices formed by arrays of micron-size projections that can pass through the stratum corneum and epidermis without reaching the nerves of the dermis layer [6]. Thus, they are a self-administered, minimally invasive method, well tolerated by human patients in proof of concept trials [7,8], that can directly target the skin immune cells [9].Several types of MNs have been developed for the delivery of antigens: solid, coated, hollow, and dissolving MNs [10]. Among them, and based on their self-disabling properties, dissolving MNs (MNs) have been selected for this work. These MNs are made of a soluble matrix, generally a biocompatible polymer or polysaccharide, which includes the antigenic complex. After the array insertion into the skin, the needle tips dissolve and the antigenic cargo i...

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“…A lot of research [10][11][12] has been geared toward the understanding of this challenge, but the complexity of it requires a different dimension of approach. 13 An approach that must accommodate many factors affecting vaccine development like pathogen antigenic variability, the emergence of infectious disease, human genetic variation is the goal of immunoinformatics [ Figure 1].…”

Section: Vaccine Immunology and Designmentioning

confidence: 99%

<p>Immunoinformatics and Vaccine Development: An Overview</p>

Oli

Obialor

Ifeanyichukwu

et al. 2020

ITT

157

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The use of vaccines have resulted in a remarkable improvement in global health. It has saved several lives, reduced treatment costs and raised the quality of animal and human lives. Current traditional vaccines came empirically with either vague or completely no knowledge of how they modulate our immune system. Even at the face of potential vaccine design advance, immune-related concerns (as seen with specific vulnerable populations, cases of emerging/reemerging infectious disease, pathogens with complex lifecycle and antigenic variability, need for personalized vaccinations, and concerns for vaccines' immunological safety -specifically vaccine likelihood to trigger non-antigen-specific responses that may cause autoimmunity and vaccine allergy) are being raised. And these concerns have driven immunologists toward research for a better approach to vaccine design that will consider these challenges. Currently, immunoinformatics has paved the way for a better understanding of some infectious disease pathogenesis, diagnosis, immune system response and computational vaccinology. The importance of this immunoinformatics in the study of infectious diseases is diverse in terms of computational approaches used, but is united by common qualities related to host-pathogen relationship. Bioinformatics methods are also used to assign functions to uncharacterized genes which can be targeted as a candidate in vaccine design and can be a better approach toward the inclusion of women that are pregnant into vaccine trials and programs. The essence of this review is to give insight into the need to focus on novel computational, experimental and computation-driven experimental approaches for studying of host-pathogen interactions and thus making a case for its use in vaccine development.

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Novel approaches for the design, delivery and administration of vaccine technologies

Cited by 94 publications

References 186 publications

Expression of ESAT‐6 antigen from Mycobacterium tuberculosis in broccoli: An edible plant

Expression of ESAT‐6 antigen from Mycobacterium tuberculosis in broccoli: An edible plant

Dissolving Microneedles for Intradermal Vaccination against Shigellosis

<p>Immunoinformatics and Vaccine Development: An Overview</p>

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