Novel approaches for application of biosurfactant produced by Pseudomonas aeruginosa for biocontrol of Colletotrichum capsici responsible for anthracnose disease in chilli

Lahkar, Jiumoni; Goswami, Debahuti; Deka, Suresh; Ahmed, Giasuddin

doi:10.1007/s10658-017-1252-3

Cited by 21 publications

(8 citation statements)

References 46 publications

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“…Tests at different concentrations of rhamnolipids in tomato plants against the fungus Alternaria solani showed that the minimum concentration of 1.5 mg mL −1 completely inhibited the development of the fungus 27 . Additionally, tests with rhamnolipids in chili peppers to control anthracnose disease, as a result of the fungus Colletotrichum capsici , concluded that the concentration of 0.5 mg mL −1 was sufficient to reduce the severity of the disease from 67.5% to 12.5% 18 …”

Section: Resultsmentioning

confidence: 99%

“…27 Additionally, tests with rhamnolipids in chili peppers to control anthracnose disease, as a result of the fungus Colletotrichum capsici, concluded that the concentration of 0.5 mg mL −1 was sufficient to reduce the severity of the disease from 67.5% to 12.5%. 18 The germicidal effect of sophorolipids was tested on different vegetables. The biosurfactant was used as a sanitizing solution at a concentration of 0.25 mg mL −1 , and the results showed that the treatment was effective in preventing the deterioration of chikoos (sapodilla), tomatoes, cucumbers and lemons.…”

Section: Test In Tomato Fruitsmentioning

confidence: 99%

“…The effect of sophorolipids against gray mold caused by the fungus B. cinerea was tested. 18 The sophorolipids solution was prepared using DMSO 20 g kg −1 and water (both sterile) in the concentrations of 1, 2 and 4 mg mL −1 . For the control, only 20 g kg −1 DMSO and water were used.…”

Section: Test In Tomato Fruitmentioning

confidence: 99%

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Antimicrobial activity of sophorolipids produced by Starmerella bombicola against phytopathogens from cherry tomato

et al. 2021

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BACKGROUND: Phytopathogenic microorganisms are the main cause of plant diseases, generating significant economic losses for the agricultural and food supply chain. Cherry tomatoes (Solanum lycopersicum var. cerasiforme) are very perishable plants and highly demanding in the use of pesticides; therefore, alternative solutions such as biosurfactants have aroused as a potent substituent. The main objective of the present study was to investigate the antimicrobial activity of sophorolipids against the phytopathogens Botrytis cinerea, Sclerotium rolfsii, Rhizoctonia solani and Pythium ultimum. RESULTS:The biosurfactant inhibited the mycelial growth in vitro with a minimum concentration of 2 mg mL −1 . The application of sophorolipids at 1, 2 and 4 mg mL −1 in detached leaves of tomato before the inoculation of the fungus B. cinerea was the best treatment, reducing leaf necrosis by up to 76.90%. The use of sophorolipids for washing tomato fruits before the inoculation of B. cinerea was able to inhibit the development of gray mold by up to 96.27%. CONCLUSION:The results for tomato leaves and fruits revealed that the biosurfactant acts more effectively when used preventively. Sophorolipids are stable molecules that show promising action for the potential replacement of pesticides in the field and the post-harvest process against the main tomato phytopathogens.

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Section: Resultsmentioning

confidence: 99%

Section: Test In Tomato Fruitsmentioning

confidence: 99%

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Antimicrobial activity of sophorolipids produced by Starmerella bombicola against phytopathogens from cherry tomato

et al. 2021

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“…Both techniques used by us have demonstrated a dose-dependent manner antifungal effect of RH-BS, FCH, and FCHNPs against the selected fungi (FI, FII, and FIII). The literature documents the noteworthy antifungal activity of RH-BS without harming the soil ecology unlike synthetic fungicides ( Lahkar et al, 2018 ). Crouzet et al (2020) reviewed the potential of RH-BS in a sustained agriculture framework suggesting the enhancement in plant immunity (local/systemic).…”

Section: Discussionmentioning

confidence: 99%

Synergistic Activity of Rhamnolipid Biosurfactant and Nanoparticles Synthesized Using Fungal Origin Chitosan Against Phytopathogens

Karamchandani

Maurya

Dalvi

et al. 2022

Front. Bioeng. Biotechnol.

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Phytopathogens pose severe implications in the quantity and quality of food production by instigating several diseases. Biocontrol strategies comprising the application of biomaterials have offered endless opportunities for sustainable agriculture. We explored multifarious potentials of rhamnolipid-BS (RH-BS: commercial), fungal chitosan (FCH), and FCH-derived nanoparticles (FCHNPs). The high-quality FCH was extracted from Cunninghamella echinulata NCIM 691 followed by the synthesis of FCHNPs. Both, FCH and FCHNPs were characterized by UV-visible spectroscopy, DLS, zeta potential, FTIR, SEM, and Nanoparticle Tracking Analysis (NTA). The commercial chitosan (CH) and synthesized chitosan nanoparticles (CHNPs) were used along with test compounds (FCH and FCHNPs). SEM analysis revealed the spherical shape of the nanomaterials (CHNPs and FCHNPs). NTA provided high-resolution visual validation of particle size distribution for CHNPs (256.33 ± 18.80 nm) and FCHNPs (144.33 ± 10.20 nm). The antibacterial and antifungal assays conducted for RH-BS, FCH, and FCHNPs were supportive to propose their efficacies against phytopathogens. The lower MIC of RH-BS (256 μg/ml) was observed than that of FCH and FCHNPs (>1,024 μg/ml) against Xanthomonas campestris NCIM 5028, whereas a combination study of RH-BS with FCHNPs showed a reduction in MIC up to 128 and 4 μg/ml, respectively, indicating their synergistic activity. The other combination of RH-BS with FCH resulted in an additive effect reducing MIC up to 128 and 256 μg/ml, respectively. Microdilution plate assay conducted for three test compounds demonstrated inhibition of fungi, FI: Fusarium moniliforme ITCC 191, FII: Fusarium moniliforme ITCC 4432, and FIII: Fusarium graminearum ITCC 5334 (at 0.015% and 0.020% concentration). Furthermore, potency of test compounds performed through the in vitro model (poisoned food technique) displayed dose-dependent (0.005%, 0.010%, 0.015%, and 0.020% w/v) antifungal activity. Moreover, RH-BS and FCHNPs inhibited spore germination (61–90%) of the same fungi. Our efforts toward utilizing the combination of RH-BS with FCHNPs are significant to develop eco-friendly, low cytotoxic formulations in future.

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“…Biosurfactants are produced by a wide range of microorganisms (Rahman and Gakpe 2008) and present several advantages: they are less toxic than chemical surfactants, and more biodegradable, biocompatible (Nguyen et al 2010;Franzetti et al 2012;De Oliveira et al 2017) and stable in extreme conditions of salinity, temperature and pH (Khopade et al 2012;Liwarska-Bizukojc et al 2018). The spectrum of applications includes petroleum exploration and bioremediation (Zhao et al 2018), control of biofilms and biocorrosion (Parthipan et al 2018), pharmaceuticals and cosmetics (Ferreira et al 2017), food processing (Kiran et al 2017), biopesticides (Lahkar et al 2018) and detergents (Lee et al 2018). However, the widespread application of BSF is still limited by high production costs.…”

Section: Introductionmentioning

confidence: 99%

Increase in bacterial biosurfactant production by co‐cultivation with biofilm‐forming bacteria

Alves

Sequeira

2019

Lett Appl Microbiol

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Considering that bacterial biosurfactants (BSFs) are released as secondary metabolites involved in biotic relations within mixed bacterial assemblages, the hypothesis that the co‐cultivation of BSF producing bacteria with biofilm‐forming strains would enhance BSF synthesis was tested. Environmental BSF producing strains of Bacillus licheniformis and Pseudomonas sp. were cultivated with reference biofilm‐forming strains (Pseudomonas aeruginosa and Listeria innocua). BSF production and quorum‐quenching effects were tested in solid media. Tensioactive and anionic BSFs were also quantified in cell‐free extracts (CFEs). BSF production increased in co‐cultures with inducer strains although this was not demonstrated by all screening methods. Increased concentrations of anionic BSF were detected in CFEs of co‐cultures in which Pseudomonas aeruginosa was included as inducer, which is in accordance with the observation of larger halos in cetyl trimethylammonium bromide‐methylene blue agar. The results demonstrate that co‐cultivation positively affects the efficiency of BSF production and that higher production yields may be attained by selecting convenient inducer partners in designed consortia. Significance and Impact of the Study The high production cost of biosurfactants (BSFs) still represents a major limitation to the industrial use of these otherwise advantageous alternatives to chemical surfactants. This work demonstrates that the co‐cultivation of consortia of biosurfactant‐producer and biofilm‐forming bacteria enhances BSF production and may contribute to the cost‐effectiveness of biosurfactant‐based products.

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Novel approaches for application of biosurfactant produced by Pseudomonas aeruginosa for biocontrol of Colletotrichum capsici responsible for anthracnose disease in chilli

Cited by 21 publications

References 46 publications

Antimicrobial activity of sophorolipids produced by Starmerella bombicola against phytopathogens from cherry tomato

Antimicrobial activity of sophorolipids produced by Starmerella bombicola against phytopathogens from cherry tomato

Synergistic Activity of Rhamnolipid Biosurfactant and Nanoparticles Synthesized Using Fungal Origin Chitosan Against Phytopathogens

Increase in bacterial biosurfactant production by co‐cultivation with biofilm‐forming bacteria

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