Novel approach towards aligned PCL-Collagen nanofibrous constructs from a benign solvent system

Dippold, Dirk; Cai, Aijia; Hardt, Moritz; Boccaccını, Aldo R.; Horch, Raymund E.; Beier, Justus P.; Schubert, Dirk W.

doi:10.1016/j.msec.2016.11.045

Cited by 48 publications

(38 citation statements)

References 22 publications

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“…Despite the wide variety of scaffold technologies available to tissue engineers today, a compromise must often be made between the desirable cell adhesion and remodeling responses of natural polymers (like triple helical collagen), and the ease of production, mechanical robustness, and molecular durability of synthetic polymers, which have rapidly advanced novel fabrication technologies like 3D printing and electrospinning. In many cases, the solution takes the form of natural and synthetic copolymers and blends, such as polycaprolactone-collagen, collagen/polylactic acid, and collagen/poly(lactic-co-glyocolic acid), [57][58][59][60] to benefit from the mechanical integrity of synthetics and the cell-binding sites of collagen. The distinct advantages of incorporating wet-spun collagen microfibers into scaffolds presented herein are that it offers precise, customizable structural control and physiologically relevant mechanical robustness without any compromise in terms of cell adhesion site density, remodeling response, and immune response by simply using unadulterated collagen in both a structurally reinforcing fiber compartment and bulk hydrogel.…”

Section: Discussionmentioning

confidence: 99%

Digital Design and Automated Fabrication of Bespoke Collagen Microfiber Scaffolds

Kaiser

Bellows

Kant

et al. 2019

Tissue Engineering Part C: Methods

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A great variety of natural and synthetic polymer materials have been utilized in soft tissue engineering as extracellular matrix (ECM) materials. Natural polymers, such as collagen and fibrin hydrogels, have experienced especially broad adoption due to the high density of cell adhesion sites compared to their synthetic counterparts, ready availability, and ease of use. However, these and other hydrogels lack the structural and mechanical anisotropy that define the ECM in many tissues, such as skeletal and cardiac muscle, tendon, and cartilage. Herein, we present a facile, low-cost, and automated method of preparing collagen microfibers, organizing these fibers into precisely controlled mesh designs, and embedding these meshes in a bulk hydrogel, creating a composite biomaterial suitable for a wide variety of tissue engineering and regenerative medicine applications. With the assistance of custom software tools described herein, mesh patterns are designed by a digital graphical user interface and translated into protocols that are executed by a custom mesh collection and organization device. We demonstrate a high degree of precision and reproducibility in both fiber and mesh fabrication, evaluate single fiber mechanical properties, and provide evidence of collagen self-assembly in the microfibers under standard cell culture conditions. This work offers a powerful, flexible platform for the study of tissue engineering and cell material interactions, as well as the development of therapeutic biomaterials in the form of custom collagen microfiber patterns that will be accessible to all through the methods and techniques described here.

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Section: Discussionmentioning

confidence: 99%

Digital Design and Automated Fabrication of Bespoke Collagen Microfiber Scaffolds

Kaiser

Bellows

Kant

et al. 2019

Tissue Engineering Part C: Methods

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“…Methods of tissue engineering and regenerative medicine [37][38][39][40][41][42][43][44][45][46][47][48][49][50][51] to circumvent the use of autologous tissue and their inherent donor site morbidity seem promising but are not clinically available for these problems yet. Although a considerable number of local or free flaps has been successfully described to surgically reconstruct these defects [7,[52][53][54][55][56][57][58] each individual case needs the optimal indication for the most suitable flap procedure.…”

Section: Discussionmentioning

confidence: 99%

When free flaps are not the first choice: is the distally based peroneus brevis still an option for foot and ankle reconstruction in the era of microsurgery?

Horch¹,

Ludolph²,

Schmitz³

et al. 2018

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“…Generation of skeletal muscle tissue and differentiation can be supported by providing an optimal 3D structure for guiding the tissue growth and allowing matrix deposition and cellular interactions. By means of electrospinning of parallel aligned fibers and further cocultivation of myoblasts and MSCs on this scaffold, Beier and colleagues [43,44] recently created favorable conditions for further scaffold-based muscle tissue engineering studies in the AV loop model (Fig. 2).…”

Section: The Av Loop As a Tool To Generate A Multitude Of Different Tmentioning

confidence: 99%

The Arteriovenous Loop: Engineering of Axially Vascularized Tissue

et al. 2018

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Background: Most of the current treatment options for large-scale tissue defects represent a serious burden for the patients, are often not satisfying, and can be associated with significant side effects. Although major achievements have already been made in the field of tissue engineering, the clinical translation in case of extensive tissue defects is only in its early stages. The main challenge and reason for the failure of most tissue engineering approaches is the missing vascularization within large-scale transplants. Summary: The arteriovenous (AV) loop model is an in vivo tissue engineering strategy for generating axially vascularized tissues using the own body as a bioreactor. A superficial artery and vein are anastomosed to create an AV loop. This AV loop is placed into an implantation chamber for prevascularization of the chamber inside, e.g., a scaffold, cells, and growth factors. Subsequently, the generated tissue can be transplanted with its vascular axis into the defect site and anastomosed to the local vasculature. Since the blood supply of the growing tissue is based on the AV loop, it will be immediately perfused with blood in the recipient site leading to optimal healing conditions even in the case of poorly vascularized defects. Using this tissue engineering approach, a multitude of different axially vascularized tissues could be generated, such as bone, skeletal or heart muscle, or lymphatic tissues. Upscaling from the small animal AV loop model into a preclinical large animal model could pave the way for the first successful attempt in clinical application. Key Messages: The AV loop model is a powerful tool for the generation of different axially vascularized replacement tissues. Due to minimal donor site morbidity and the possibility to generate patient-specific tissues variable in type and size, this in vivo tissue engineering approach can be considered as a promising alternative therapy to current treatment options of large-scale defects.

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Novel approach towards aligned PCL-Collagen nanofibrous constructs from a benign solvent system

Cited by 48 publications

References 22 publications

Digital Design and Automated Fabrication of Bespoke Collagen Microfiber Scaffolds

Digital Design and Automated Fabrication of Bespoke Collagen Microfiber Scaffolds

When free flaps are not the first choice: is the distally based peroneus brevis still an option for foot and ankle reconstruction in the era of microsurgery?

The Arteriovenous Loop: Engineering of Axially Vascularized Tissue

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