Novel Approach to Tooth Chemistry: Quantification of Human Enamel Apatite in Context for New Biomaterials and Nanomaterials Development

Kuczumow, Andrzej; Chałas, Renata; Nowak, Jakub; Smułek, Wojciech; Jarzębski, Maciej

doi:10.3390/ijms22010279

Cited by 11 publications

(10 citation statements)

References 86 publications

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“…Meanwhile, the concentration of Cl is inversely correlated with Mg. This provides a striking confirmation of our previous studies, as presented in [26,38], where it was shown that the apatite matter in enamel and its variability are strongly stoichiometric. Now, this finding has widened to encompass water and organic matter.…”

Section: Discussionsupporting

confidence: 91%

“…All the mentioned relationships have positive correlations, which is very important from the point of view of Mg's role in the formation of enamel. Moreover, changes in Mg contents correspond to the well-correlated changes in Cl concentrations, which were previously and independently indicated in our earlier papers [26,38] and was found to have important implications for the ordered ion exchanges in enamel (see Figures 3 and 4). There exists a strong linear and positive correlation between the growth in conten of organic ma er and magnesium (see simple graph in Figure 2 showing the direction chemical variability).…”

Section: Resultssupporting

confidence: 79%

“…Their role has been extensively studied for years; however, it has not been fully explained until now [23][24][25]. The spatial distribution of elements inside enamel has been intensively studied, with proven regularity and stoichiometry in the direction: from the dental-enamel junction to the enamel-air boundary [26][27][28]. It became clear that the presence of water, organic matter, and minor elements shaped the specific features of the enamel, differentiating it from pure hydroxyapatite.…”

Section: Introductionmentioning

confidence: 99%

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Contribution to Knowledge on Bioapatites: Does Mg Level Reflect the Organic Matter and Water Contents of Enamel?

Lasota,

Kuczumow,

Gorzelak

et al. 2023

IJMS

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The matter constituting the enamels of four types of organisms was studied. The variability of the ions was presented in molar units. It was proven that the changes in water contents of the enamel are significantly positively related to changes in Mg; inversely, there is also a strong connection with changes in Ca and P, the main components of bioapatite. The variability in the organic matter has the same strong and positive characteristics and is also coupled with changes in Mg contents. Amelogenins in organic matter, which synthesize enamel rods, likely have a role in adjusting the amount of Mg, thus establishing the amount of organic matter and water in the whole enamel; this adjustment occurs through an unknown mechanism. Ca, P, Mg, and Cl ions, as well as organic matter and water, participate in the main circulation cycle of bioapatites. The selection of variations in the composition of bioapatite occurs only along particular trajectories, where the energy of transformation linearly depends on the following factors: changes in the crystallographic d parameter; the increase in the volume, V, of the crystallographic cell; the momentum transfer, which is indirectly expressed by ΔsinΘ value. To our knowledge, these findings are novel in the literature. The obtained results indicate the different chemical and crystallographic affinities of the enamels of selected animals to the human ones. This is essential when animal bioapatites are transformed into dentistic or medical substitutes for the hard tissues. Moreover, the role of Mg is shown to control the amount of water in the apatite and in detecting organic matter in the enamels.

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Section: Discussionsupporting

confidence: 91%

Section: Resultssupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Contribution to Knowledge on Bioapatites: Does Mg Level Reflect the Organic Matter and Water Contents of Enamel?

Lasota,

Kuczumow,

Gorzelak

et al. 2023

IJMS

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“…By using the procedure and equations we previously described from this series [40,41] for parameter "a",…”

Section: Energetic Relationshipsmentioning

confidence: 99%

Novel Approach to Tooth Chemistry. Quantification of the Dental-Enamel Junction

Kuczumow¹,

Chałas

Nowak³

et al. 2021

IJMS

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The dentin-enamel junction (DEJ) is known for its special role in teeth. Several techniques were applied for the investigation of the DEJ in human sound molar teeth. The electron (EPMA) and proton (PIXE) microprobes gave consistent indications about the variability of elemental concentrations on this boundary. The locally increased and oscillating concentrations of Mg and Na were observed in the junction, in the layer adhering to the enamel and covering roughly half of the DEJ width. The chemical results were compared with the optical profiles of the junction. Our chemical and optical results were next compared with the micromechanical results (hardness, elastic modulus, friction coefficient) available in the world literature. A strong correlation of both result sets was proven, which testifies to the self-affinity of the junction structures for different locations and even for different kinds of teeth and techniques applied for studies. Energetic changes in tooth strictly connected with crystallographic transformations were calculated, and the minimum energetic status was discovered for DEJ zone. Modeling of both walls of the DEJ from optical data was demonstrated. Comparing the DEJ in human teeth with the same structure found in dinosaur, shark, and alligator teeth evidences the universality of dentin enamel junction in animal world. The paper makes a contribution to better understanding the joining of the different hard tissues.

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“…2. The fact that the structural characteristics of the specimens remained unchanged without the evidence of producing FA/FHA suggests that the newly formed structure inside the lesion was mainly composed of HA incorporated with substituent ions from labile minerals at the mineralizing zone—for example, Mg 2+ /Na + for Ca 2+ , F − /CO 3 2− for OH − /HPO 4 2− , and CO 3 2− for PO 4 3− (substitution B) with Na + for the rearrangement of electrical charges (Hughes 1996; Kuczumow et al 2020). The undisturbed quality of crystals detected after AIP treatment is probably due to the high carbonate content in their structure (Sa et al 2014; Abou Neel et al 2016), as it is well documented that biological apatite like dental enamel could be modified by other constituents without affecting their basic phases (Antonakos et al 2007; Buchalla 2013).…”

Section: Discussionmentioning

confidence: 99%

Enamel Remineralization and Crystallization after Fluoride Iontophoresis

et al. 2022

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Early caries lesions consist of noncavitated subsurface demineralization caused by the dissolution of hydroxyapatite from the surface to the subsurface area of the enamel. Such lesions cannot be remineralized effectively by the conventional treatment. Thus, there is a need for a noninvasive technique capable of delivering the remineralizing agent to subsurface sites. For this purpose, fluoride iontophoresis (IP) using weak currents has been investigated with some conflicting results and no information on the crystal structure and composition. Because enamel remineralization involves the role of fluid from dentin, the presence of enamel fluid is necessary to determine the repair associated with the physiological condition. This study aimed to investigate structural and compositional characteristics, including the remineralizing effect of 5% sodium fluoride (NaF) IP with different polarities, cathodal iontophoresis (CIP), and anodal iontophoresis (AIP) for the treatment of natural enamel caries under simulated pulpal pressure. A bulk measurement of the crystal structure inside the lesion was first determined using calcium (Ca) K-edge X-ray absorption spectroscopy. IP with both polarities significantly promoted subsurface remineralization. The CIP generated a significant increase in the Ca/phosphorus ratio, and fluoride at the surface lesion significantly correlated with higher mineral density (MD) and more strengthening crystal structure of the lesion volume, while the lesion’s MD and other impurities at the lesion surface, mostly the carbonate ions, affected the significant increase in MD with the unchanged structure of the lesion volume after AIP. The CIP of NaF is an ideal method for rapid enamel remineralization and recrystallization of fluoroapatite/fluorohydroxyapatite.

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Novel Approach to Tooth Chemistry: Quantification of Human Enamel Apatite in Context for New Biomaterials and Nanomaterials Development

Cited by 11 publications

References 86 publications

Contribution to Knowledge on Bioapatites: Does Mg Level Reflect the Organic Matter and Water Contents of Enamel?

Contribution to Knowledge on Bioapatites: Does Mg Level Reflect the Organic Matter and Water Contents of Enamel?

Novel Approach to Tooth Chemistry. Quantification of the Dental-Enamel Junction

Enamel Remineralization and Crystallization after Fluoride Iontophoresis

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