Novel approach to the determination of structurally similar hydroxamate siderophores by column-switching capillary liquid chromatography coupled to mass spectrometry

Moberg, My; Holmström, Sara; Lundström, Ulla; Markides, Karin E.

doi:10.1016/s0021-9673(03)01236-6

Cited by 25 publications

(19 citation statements)

References 15 publications

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“…Quantification of the siderophores was performed via column-switching capillary HPLC-ESI-MS based upon the method developed by Moberg et al (2003). The HPLC-system was built up of two microflow pumps (Perkin Elmer 200 Micro Series), both operated at 10 ll/min, and an auto-injector equipped with a thermostat (Agilent 1100, G1367A WPALS and G1330 ALSTherm, respectively).…”

Section: Quantitative Analysis By Capillary Hplc-msmentioning

confidence: 99%

“…Combining chromatographic separation with selective mass spectrometry (MS) detection allows analysis and quantification of ferrichrome analogues in soil solution with a detection limit for ferrichrome of approximately 1 nM , although ferrichrome and ferricrocin were not chromatographically separated. A method developed by Moberg et al (2003), using on-line preconcentration by column-switching and separation on porous graphitic carbon column, enables separation of ferrichrome, ferricrocin and ferrichrysin with detection limits of 28-46 pM obtained by MS detection (Moberg et al 2003). Via this switching technique, soil solution samples can be analysed without any prior pre-concentration or clean-up.…”

Section: Introductionmentioning

confidence: 99%

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Quantification of Hydroxamate Siderophores in Soil Solutions of Podzolic Soil Profiles in Sweden

et al. 2006

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Concentrations up to 2 and 12 nM of the hydroxamate siderophores ferrichrome and ferricrocin, respectively, were identified in soil solutions of podzolic forest soils at four sites in both northern and southern Sweden. No ferrichrysin was detected. As with the dissolved organic carbon and low molecular mass organic acids, the highest concentrations of the siderophores were found in the upper layers i.e. the mor layer, the eluvial and upper illuvial horizons. At the southern sites, the concentrations of ferrichrome and ferricrocin were both of similar magnitude and did not differ between the two sites. In contrast, soil solutions at the two northern sites contained more ferricrocin than ferrichrome; the ferricrocin concentrations were also higher at the northern sites than at the southern sites. Analyses were performed by high performance liquid chromatography with a porous graphitic carbon column on which ferrichrome, ferricrocin and ferrichrysin were separated. Detection by electrospray ionization mass spectrometry (ESI-MS) combined with on-line sample pre-concentration, by means of column-switching, enabled detection limits of 0.1-0.2 nM for ferrichrome, ferrichrysin and ferricrocin. The structural identities of the siderophores were further verified by MS/MS fragmentation. Fragmentation of ferrichrome, ferricrocin and ferrichrysin occurred mainly via peptide cleavage. The most intense fragments were typified by the loss of one of the three iron(III) chelating hydroxamate residues, i.e N(5)-acyl-N(5)-hydroxy ornithine.

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Section: Quantitative Analysis By Capillary Hplc-msmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantification of Hydroxamate Siderophores in Soil Solutions of Podzolic Soil Profiles in Sweden

et al. 2006

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“…Earlier studies that determined the siderophores by microbial assays were very specific but had low a sensitivity (e.g Buyer et al 1993), while recently HPLC-ESI-MS methods has been used intensively since it gives more accurate and highly sensitive results (Moberg et al, 2003;Haselwandter et al, 2013).…”

Section: Factors Influencing the Siderophore Production By The Isolatmentioning

confidence: 99%

Siderophore Production by Microorganisms Isolated From a Podzol Soil Profile

Ahmed

Holmström

2014

Geomicrobiology Journal

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Siderophore producing bacteria/actinobacteria and fungi were isolated from O-(organic), E-(eluvial), B-(upper illuvial), and C-(parent material) horizons of podzol soil. Siderophores were isolated and hydroxamate type siderophores were detected and quantitated by high-performance liquid chromatography coupled to electrospray ionization mass spectrometry. The molecular identification of siderophore producing isolates showed that there was a high diversity of fungal and bacterial/actinobacterial species throughout the soil profile. The isolated bacteria/actinobacteria showed different abilities in the production of ferrioxamines (E, B, G and D). Moreover, the isolated fungal species showed great variety in the production of ferrichromes, coprogens and fusarinines.

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“…By applying column-switching, analytes can be preconcentrated on-line [15] and this technique has been used for the quantification of ferrichrome, ferrichrysin and ferricrocin in soil solution, applying MS detection [15,16]. In their work, Essén et al [16] also included MS/MS fragmentation of the three ferrichromes for structural identification.…”

Section: Introductionmentioning

confidence: 99%

Analysis of hydroxamate siderophores in soil solution using liquid chromatography with mass spectrometry and tandem mass spectrometry with on-line sample preconcentration

Olofsson

Bylund

2015

J. Sep. Science

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A liquid chromatography with electrospray ionization mass spectrometry method was developed to quantitatively and qualitatively analyze 13 hydroxamate siderophores (ferrichrome, ferrirubin, ferrirhodin, ferrichrysin, ferricrocin, ferrioxamine B, D1 , E and G, neocoprogen I and II, coprogen and triacetylfusarinine C). Samples were preconcentrated on-line by a switch-valve setup prior to analyte separation on a Kinetex C18 column. Gradient elution was performed using a mixture of an ammonium formate buffer and acetonitrile. Total analysis time including column conditioning was 20.5 min. Analytes were fragmented by applying collision-induced dissociation, enabling structural identification by tandem mass spectrometry. Limit of detection values for the selected ion monitoring method ranged from 71 pM to 1.5 nM with corresponding values of two to nine times higher for the multiple reaction monitoring method. The liquid chromatography with mass spectrometry method resulted in a robust and sensitive quantification of hydroxamate siderophores as indicated by retention time stability, linearity, sensitivity, precision and recovery. The analytical error of the methods, assessed through random-order, duplicate analysis of soil samples extracted with a mixture of 10 mM phosphate buffer and methanol, appears negligible in relation to between-sample variations.

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Novel approach to the determination of structurally similar hydroxamate siderophores by column-switching capillary liquid chromatography coupled to mass spectrometry

Cited by 25 publications

References 15 publications

Quantification of Hydroxamate Siderophores in Soil Solutions of Podzolic Soil Profiles in Sweden

Quantification of Hydroxamate Siderophores in Soil Solutions of Podzolic Soil Profiles in Sweden

Siderophore Production by Microorganisms Isolated From a Podzol Soil Profile

Analysis of hydroxamate siderophores in soil solution using liquid chromatography with mass spectrometry and tandem mass spectrometry with on-line sample preconcentration

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