Novel approach to sintering hydroxyapatite-alumina nanocomposites at 300 °C

Akmal, Muhammad; Hassan, Muhmood ul; Afzal, Muhammad; Ryu, Ho Jin

doi:10.1016/j.matchemphys.2020.124187

Cited by 13 publications

(7 citation statements)

References 29 publications

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“…The post-enzyme-treated samples were further used in the protein identification through SDS−PAGE. 56 An 8% gel was used to conduct the SDS−PAGE analysis of the detached protein samples to identify the target and nontarget proteins. In Figure 6a, thrombin, IgE and mCardinal2 in the outlet samples collected from each lane of the gel are compared with a standard protein marker in lane 1 of the gel.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…These outlet samples were treated with DNase enzyme, which hydrolyzed the single-stranded DNAs of apt15 and D17.4 and released the target thrombin and IgE proteins from the PS6–apt15–th and PS7–aptD17.4–IgE complexes, respectively. The post-enzyme-treated samples were further used in the protein identification through SDS–PAGE . An 8% gel was used to conduct the SDS–PAGE analysis of the detached protein samples to identify the target and nontarget proteins.…”

Section: Resultsmentioning

confidence: 99%

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Acoustofluidic Separation of Proteins Using Aptamer-Functionalized Microparticles

et al. 2021

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We propose an acoustofluidic method for the triseparation of proteins conjugated with aptamer-coated microparticles inside a microchannel. Traveling surface acoustic waves (TSAWs) produced from a slanted-finger interdigital transducer (SFIT) are used to separate the protein-loaded microparticles of different sizes via the TSAW-driven acoustic radiation force (ARF). The acoustofluidic device consists of an SFIT deposited onto a piezoelectric lithium niobate substrate and a polydimethylsiloxane (PDMS) microfluidic channel on top of the substrate. The TSAWs propagating on the substrate penetrate into the sample fluid flow, where the human protein-conjugated microparticles are suspended, inside the PDMS microchannel. The microparticles are subjected to the TSAW-driven ARF with varying magnitude depending on their size and thus flow along different streamlines, leading to triseparation of the proteins. In this work, we used two different-sized streptavidin-functionalized polystyrene (PS) microparticles to capture two kinds of aptamers (apt15 and aptD17.4), which were labeled with a respective biotin molecule at one end. The biotin ends of the aptamers were attached to the microparticles through streptavidin–biotin linkage, whereas the free ends of the aptamers were used to capture their target proteins of thrombin (th) and immunoglobulin E (IgE). The resultant PS–apt15–th and PS–aptD17.4–IgE complexes, as well as mCardinal2, were used for experimental demonstration of acoustofluidic triseparation of the human proteins. We achieved simultaneous separation of proteins of three kinds (th, IgE, and mCardinal2) for the first time via the TSAW-driven ARF in the proposed acoustofluidic device.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Acoustofluidic Separation of Proteins Using Aptamer-Functionalized Microparticles

et al. 2021

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“…In vitro human osteoblast-like MG-63 cell culture, adhesion, and proliferation [50] Nanoporous α-alumina -Cell adhesion, viability, and osteogenic potential pre-osteoblastic MC3T3-E1 cell [51] Porous alumina Calcium phosphate and strontium ion - [52] α-alumina particle Hydroxyapatite/poly-εcaprolactone composite Dermal fibroblast normal human, neonatal (HDFn) cell culture and viability [53] Porous α-alumina Fluorapatite + TiO 2 composite - [54] Porous alumina Coated with platinum, titanium, and tantalum Cell behavior fibroblast and hematopoietic stem cell (HSC) [55] Nano alumina powder Hydroxyapatite Cell culture and plasma protein adsorption [56] Porous alumina Hydroxyapatite - [57] Nano γ-alumina Silk fibroin/chitosan composite In vitro biodegradation and biomineralization [58] stability in wet physiological environments [41,42]. These factors are important because the human body possesses a highly corrosive environment.…”

Section: Composite With Hydroxyapatitementioning

confidence: 99%

“…Thus, the authors concluded that alumina nanowires influenced cellular behavior, and improved the bioactivity and the mechanical properties of the scaffold. Unlike the bulk form, nano alumina has shown bioactive nature [56]. This evidences its importance as a biomaterial for tissue engineering.…”

Section: Porous α-Alumina and α-Alumina Compositesmentioning

confidence: 99%

Alumina applied in bone regeneration: Porous α-alumina and transition alumina

et al. 2022

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Alumina is a polymorphic bioceramic that has been extensively investigated for application in bone regeneration. Dense α-alumina has been considered a suitable biomaterial for dental and orthopedic implants due to its superior mechanical properties. However, its use is limited due to its high inertia in a biological environment. Recent investigations have focused on its distinct phases and surface characteristics through the control of morphology, physical properties, and chemical composition to enhance bioactivity. This article presents a brief review of the developments in porous α-alumina and amorphous-γ-alumina transition. Most studies have shown that composites and alumina coated with bioactive materials, high surface area, and hydroxylated surface can significantly improve biological properties. Cellular responses such as fixation, growth, and proliferation, as well as biomineralization, are the main studies to validate improvements in bioactive properties.

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“…reported that amorphous calcium phosphate (ACP) can be sintered to near full density only at room temperature. Akmal et al 19 . prepared HA‐alumina nanocomposites with significant hardness at 300°C.…”

Section: Introductionmentioning

confidence: 99%

Effects of water on cold‐sintered highly dense dicalcium phosphate anhydrous bioceramic using its hydrate

Wang,

Zhang,

et al. 2024

J Am Ceram Soc.

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The dicalcium phosphate anhydrous (DCPA, CaHPO4) bioceramic has long been difficult to prepare by conventional sintering techniques due to its weak thermal stability. Here, the DCPA ceramic is prepared at 250°C by cold sintering process (CSP) using the dicalcium phosphate dihydrate (DCPD, CaHPO4·2H2O) as the starting powder. It is found that the dehydration temperature of DCPD powder is greatly reduced during CSP, which facilitates the densification even without additive water. However, the ceramic only reaches a relatively low density of around 90% without additive water, which can be ascribed to the lack of particle rearrangement and the readily loss of dehydration water during sintering. In comparison, a high relative density of 95% can be obtained when the DCPD hydrate is densified with additive water, in which both hydration water and additive water play important roles. The obtained DCPA ceramic with high density exhibits a flexural strength of 46.17 MPa and Young's modulus closer to human bones compared with hydroxyapatite. Combined with the low cytotoxicity, the DCPA bioceramic has great potential to be applied for bone tissue engineering.

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Novel approach to sintering hydroxyapatite-alumina nanocomposites at 300 °C

Cited by 13 publications

References 29 publications

Acoustofluidic Separation of Proteins Using Aptamer-Functionalized Microparticles

Acoustofluidic Separation of Proteins Using Aptamer-Functionalized Microparticles

Alumina applied in bone regeneration: Porous α-alumina and transition alumina

Effects of water on cold‐sintered highly dense dicalcium phosphate anhydrous bioceramic using its hydrate

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