Novel approach to gastric mucosal defect repair using fresh amniotic membrane allograft in dogs (experimental study)

Farghali, Haithem A.; AbdElKader, Naglaa A.; Khattab, Marwa S.; AbuBakr, Huda O.

doi:10.1186/s13287-017-0682-3

Cited by 9 publications

(7 citation statements)

References 46 publications

(48 reference statements)

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“…wt. ; Ketalar, Sigma-Aldrich Co.) and maintained by ketamine HCl 18 . In the TSP group, the treatment was performed according to previously described 15 .…”

Section: Methodsmentioning

confidence: 99%

The implication of autoantibodies in early diagnosis and monitoring of plasmonic photothermal therapy in the treatment of feline mammary carcinoma

El-Rasikh

Farghali

Abdelrahman

et al. 2021

Sci Rep

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Feline mammary carcinoma (FMC) shows great similarities to human breast cancer in the cellular and molecular levels. So, in cats as in humans, the role of immune responses is indicated to detect and follow up the development of tumors. As a new breast cancer therapeutic approach, Plasmonic Photothermal Therapy (PPTT) is an effective localized treatment for canine and feline mammary-carcinoma. Its systemic effect has not been inquired yet and needs many studies to hypothesis how the PPTT eradicates tumor cells. In this study, it is the first time to detect (P53, PCNA, MUC-1, and C-MYC) feline autoantibodies (AAbs), study the relationship between PCNA AAbs and mammary-tumors, and investigate the effect of PPTT on the humoral immune response of cats with mammary-carcinoma through detection of AAbs level before, during, and after the treatment. The four-AAbs panel was evaluated in serum of normal and clinically diagnosed cats with mammary tumors using Enzyme-Linked Immunosorbent Assay. The panel showed 100% specificity and 93.7% sensitivity to mammary tumors. The panel was evaluated in PPTT monotherapy, mastectomy monotherapy, and combination therapy. PPTT monotherapy decreased AAbs level significantly while mastectomy monotherapy and combination therapy had a nonsignificant effect on AAbs level.

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“…wt. ; Ketalar, Sigma-Aldrich Co.) and maintained by ketamine HCl 18 . In the TSP group, the treatment was performed according to previously described 15 .…”

Section: Methodsmentioning

confidence: 99%

The implication of autoantibodies in early diagnosis and monitoring of plasmonic photothermal therapy in the treatment of feline mammary carcinoma

El-Rasikh

Farghali

Abdelrahman

et al. 2021

Sci Rep

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“…Epithelial cells are responsible for the integrity and function of the gastric mucosal barrier [ 7 ]. The gastric mucosal barrier is a complex system involving physical, chemical, and biological defense mechanisms that protect the stomach from irritating food, gastric acid, and excessive pepsin activity [ 8 ].…”

Section: Gastric Mucosal Barriermentioning

confidence: 99%

Autophagy in Gastric Mucosa: The Dual Role and Potential Therapeutic Target

Guo

Chai

et al. 2021

BioMed Research International

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The incidence of stomach diseases is very high, which has a significant impact on human health. Damaged gastric mucosa is more vulnerable to injury, leading to bleeding and perforation, which eventually aggravates the primary disease. Therefore, the protection of gastric mucosa is crucial. However, existing drugs that protect gastric mucosa can cause nonnegligible side effects, such as hepatic inflammation, nephritis, hypoacidity, impotence, osteoporotic bone fracture, and hypergastrinemia. Autophagy, as a major intracellular lysosome-dependent degradation process, plays a key role in maintaining intracellular homeostasis and resisting environmental pressure, which may be a potential therapeutic target for protecting gastric mucosa. Recent studies have demonstrated that autophagy played a dual role when gastric mucosa exposed to biological and chemical factors. More indepth studies are needed on the protective effect of autophagy in gastric mucosa. In this review, we focus on the mechanisms and the dual role of various biological and chemical factors regulating autophagy, such as Helicobacter pylori, virus, and nonsteroidal anti-inflammatory drugs. And we summarize the pathophysiological properties and pharmacological strategies for the protection of gastric mucosa through autophagy.

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“…In the TS group, the mastectomy operations were performed as previously described [18]. ) and maintained by ketamine HCl [19]. In the TSP group, the treatment was performed according to previously described [15].…”

Section: Treatmentsmentioning

confidence: 99%

The Implication of Autoantibodies in Early Diagnosis and Monitoring of Plasmonic Photothermal Therapy in the Treatment of Feline Mammary Carcinoma

El-Rasikh

Farghali

Abdelrahman

et al. 2021

Preprint

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BackgroundLast year’s plasmonic photothermal therapy (PPTT) achieved great success in the treatment of feline mammary carcinoma and there is a demand for developing an easy, cheap, rapid, sensitive, and specific tool for early diagnosis and monitoring of treatment response.MethodsA panel of four autoantibodies (P53, PCNA, MUC-1, and C-MYC) was evaluated in serum of 11 normal cats and 34 cats diagnosed with mammary tumors before, during, and after the treatment using Enzyme-Linked Immunosorbent Assay (ELISA) assay. Out of the 34 cats, 15 did not receive any treatment, ten cats were treated with PPTT, three cats were treated by mastectomy, and six cats were treated with a combination of PPTT and surgery. ResultsThe panel showed high specificity and sensitivity to mammary tumors. The panel also was efficient in the evaluation of PPTT treatment response as their values decreased significantly after three months from the end of treatment. During the PPTT treatment course, the panel values increased within a month before the appearance of secondary tumors and/or metastasis but this increase was not statistically significant. As a result of the short follow-up period in this study, the studied panel was not valid in early diagnosis of primary and/or recurrent tumors nor the evaluation of treatment response of surgery alone or combination therapy. Cats treated with PPTT monotherapy showed the highest survival rate followed by cats treated with combination therapy then cats treated with mastectomy.ConclusionThe studied autoantibodies panel can be used to evaluate PPTT treatment response after three months of the end of treatment and may be efficient in early diagnosis of primary tumors, secondary tumors, recurrence, or metastasis.Trial registrationAll animals were handled in accordance with the Association for Assessment and Accreditation of Laboratory Animal Care and Office of Laboratory Animal Welfare guidelines. All animal experiments were approved by the Institutional Animal Care and Use Committee (CU-IACUC) Cairo University (code: CU II F 9 16). The animal experiments were carried out after the owner's permission. Written informed consent was provided by each cat owner for the treatments.

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Novel approach to gastric mucosal defect repair using fresh amniotic membrane allograft in dogs (experimental study)

Cited by 9 publications

References 46 publications

The implication of autoantibodies in early diagnosis and monitoring of plasmonic photothermal therapy in the treatment of feline mammary carcinoma

The implication of autoantibodies in early diagnosis and monitoring of plasmonic photothermal therapy in the treatment of feline mammary carcinoma

Autophagy in Gastric Mucosa: The Dual Role and Potential Therapeutic Target

The Implication of Autoantibodies in Early Diagnosis and Monitoring of Plasmonic Photothermal Therapy in the Treatment of Feline Mammary Carcinoma

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