Novel approach for the determination of azithromycin in pharmaceutical formulations by Fourier transform infrared spectroscopy in film-through transmission mode

Robaina, Nicolle F.; Paula, Carlos Eduardo R. de; Brum, Daniel M.; Guárdia, Miguel de la; Garrigues, Salvador; Cassella, Ricardo J.

doi:10.1016/j.microc.2013.04.015

Cited by 25 publications

(15 citation statements)

References 31 publications

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“…Various AZM characteristic bands could be identified in the spectra acquired straight from the solid standard, as shown in Figure 1A. The main bands identified were (i) bands associated with the axial stretching and bending of C-H of the methyl groups, which were found in the ranges of 2966-2985 cm −1 and 1367-1374 cm −1 , respectively [57,58]; (ii) the sharp and powerful band at 1710-1722 cm −1 , which could be attributed to the axial stretching of the lactone's C=O group; and (iii) other notable bands in the spectra, including those found in the 1130-1225 cm −1 region, which emerged due to absorption related to C-O axial stretching [59].…”

Section: Ft-ir Analysismentioning

confidence: 97%

“…The temperature employed during nanoparticle manufacturing affected the LE% of the drug; as the temperature increased, the LE% decreased. Because the drug-adsorption process on the surface of the ZnONPs decreased as the temperature of the AZM with the ZnONP reflux process increased, these results were expected [59]. The major goal of the temperature impact study was to find the optimal reflux temperature that would result in a greater LE percentage.…”

Section: In Vitro Release Study Of Azm-znonpsmentioning

confidence: 99%

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Tailoring of Novel Azithromycin-Loaded Zinc Oxide Nanoparticles for Wound Healing

et al. 2022

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Skin is the largest mechanical barrier against invading pathogens. Following skin injury, the healing process immediately starts to regenerate the damaged tissues and to avoid complications that usually include colonization by pathogenic bacteria, leading to fever and sepsis, which further impairs and complicates the healing process. So, there is an urgent need to develop a novel pharmaceutical material that promotes the healing of infected wounds. The present work aimed to prepare and evaluate the efficacy of novel azithromycin-loaded zinc oxide nanoparticles (AZM-ZnONPs) in the treatment of infected wounds. The Box–Behnken design and response surface methodology were used to evaluate loading efficiency and release characteristics of the prepared NPs. The minimum inhibitory concentration (MIC) of the formulations was determined against Staphylococcus aureus and Escherichia coli. Moreover, the anti-bacterial and wound-healing activities of the AZM-loaded ZnONPs impregnated into hydroxyl propyl methylcellulose (HPMC) gel were evaluated in an excisional wound model in rats. The prepared ZnONPs were loaded with AZM by adsorption. The prepared ZnONPs were fully characterized by XRD, EDAX, SEM, TEM, and FT-IR analysis. Particle size distribution for the prepared ZnO and AZM-ZnONPs were determined and found to be 34 and 39 nm, respectively. The mechanism by which AZM adsorbed on the surface of ZnONPs was the best fit by the Freundlich model with a maximum load capacity of 160.4 mg/g. Anti-microbial studies showed that AZM-ZnONPs were more effective than other controls. Using an experimental infection model in rats, AZM-ZnONPs impregnated into HPMC gel enhanced bacterial clearance and epidermal regeneration, and stimulated tissue formation. In conclusion, AZM -loaded ZnONPs are a promising platform for effective and rapid healing of infected wounds.

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Section: Ft-ir Analysismentioning

confidence: 97%

Section: In Vitro Release Study Of Azm-znonpsmentioning

confidence: 99%

Tailoring of Novel Azithromycin-Loaded Zinc Oxide Nanoparticles for Wound Healing

et al. 2022

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“…For the past two decades, the transmission FTIR spectroscopy has undergone significant application for quantitative purposes, several in the pharmaceutical analysis field 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 . As suggested above, a FTIR spectrum can be used for verifying the identity and purity of API and for detecting any spectral interference originating from the excipients.…”

Section: Introductionmentioning

confidence: 99%

Transmission FTIR derivative spectroscopy for estimation of furosemide in raw material and tablet dosage form

Gallignani

Rondon

Ovalles

et al. 2014

Acta Pharmaceutica Sinica B

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A Fourier transform infrared derivative spectroscopy (FTIR-DS) method has been developed for determining furosemide (FUR) in pharmaceutical solid dosage form. The method involves the extraction of FUR from tablets with N,N-dimethylformamide by sonication and direct measurement in liquid phase mode using a reduced path length cell. In general, the spectra were measured in transmission mode and the equipment was configured to collect a spectrum at 4 cm−1 resolution and a 13 s collection time (10 scans co-added). The spectra were collected between 1400 cm−1 and 450 cm−1. Derivative spectroscopy was used for data processing and quantitative measurement using the peak area of the second order spectrum of the major spectral band found at 1165 cm−1 (SO2 stretching of FUR) with baseline correction. The method fulfilled most validation requirements in the 2 mg/mL and 20 mg/mL range, with a 0.9998 coefficient of determination obtained by simple calibration model, and a general coefficient of variation <2%. The mean recovery for the proposed assay method resulted within the (100±3)% over the 80%–120% range of the target concentration. The results agree with a pharmacopoeial method and, therefore, could be considered interchangeable.

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“…This research is a kind of descriptive research using the Fourier Transform Infrared spectrophotometry method to identify and determine the level of metformin hydrochloride contained in marketed tablet dosages. The method used in this research included preparation of standard, analysis of sample, and validation of method with a slight modification from previous research (Robaina et al, 2013).…”

Section: Researchmentioning

confidence: 99%

Application of Fourier Transform Infrared Spectrophotometry Method for Analysis of Metformin Hydrochloride in Marketed Tablet Dosage Form

Nerdy

Margata

Meliala

et al. 2021

Valensi

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The first line drug given for monotherapy for diabetes mellitus type 2 is metformin hydrochloride, which is a biguanide antihyperglycemic drug. The aim of this research was to develop, validate, and apply the Fourier Transform Infrared spectrophotometry method to identify and determine metformin hydrochloride in marketed tablet dosage form. This research included preparation of standard, analysis of samples, and validation of method. The specific wavenumber obtained for qualitative analysis was 1645.68 cm–1 and 1574.8 cm–1. The specific area obtained for quantitative analysis with a single baseline ranged from 1701.53 cm–1 to 1535.66 cm–1. All metformin hydrochloride marketed tablet dosage forms were analyzed and met all of the qualitative and quantitative requirements. The methods met the requirements of method validation for accuracy with a percentage of recovery of 100.22 %, precision with relative standard deviation of 0.48 %, linearity with a correlation coefficient of 0.9992, limit of detection of 11.17 mg per mL, limit of quantitation of 33.84 mg per mL, and good specificity results. In this study, the Fourier Transform Infrared spectrophotometry method was successfully developed and validated for application in identification and determination of metformin hydrochloride in marketed tablet dosage form.

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Novel approach for the determination of azithromycin in pharmaceutical formulations by Fourier transform infrared spectroscopy in film-through transmission mode

Cited by 25 publications

References 31 publications

Tailoring of Novel Azithromycin-Loaded Zinc Oxide Nanoparticles for Wound Healing

Tailoring of Novel Azithromycin-Loaded Zinc Oxide Nanoparticles for Wound Healing

Transmission FTIR derivative spectroscopy for estimation of furosemide in raw material and tablet dosage form

Application of Fourier Transform Infrared Spectrophotometry Method for Analysis of Metformin Hydrochloride in Marketed Tablet Dosage Form

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