Novel Approach for Large-Scale, Biocompatible, and Low-Cost Fractionation of Peptides in Proteolytic Digest of Food Protein Based on the Amphoteric Nature of Peptides

Abstract

A large-scale, biocompatible, and low-cost procedure for peptide fractionation based on the amphoteric nature of peptide is developed. A sample cell (120 x 100 x 50 mm) with four joint tubes (17 mm i.d. and 20 mm in length) on the front and back was prepared. On the end of the joint tubes, a nylon screen (100 mesh)-supported agarose gel layer was formed. Five or nine of the sample cells were connected. A tryptic digest of casein (2.0-3.6 L) was applied to the sample cells. At each end of the sample cell apparatus, an additional cell filled with 0.1 M H(3)PO(4) or NaOH was connected and used as anode and cathode compartments, respectively. Reproducible fractionation of peptide could be achieved by collecting fractions with specific pH values when voltage reached a plateau by applying direct current at constant power. Running time necessary for fractionation of peptide was inversely proportional to electric power and directly proportional to sample volume.

Protease Treatment for the Stabilization of Rice Bran: Effects on Lipase Activity, Antioxidants, and Lipid Stability

“…The slower hydrolysis rate may be because of the decrease of substrate concentration, and its fragments can also be substrates or inhibitors. Furthermore, protein hydrolysates have been reported for their antioxidative property and for increasing calcium absorption (Akahoshi et al 2000;Chanput et al 2009). Bromelain and Flavourzyme enzyme showed a lower degree of hydrolysis than the other enzymes (P < 0.01).…”

“…Li et al (2007) reported that rice bran protein hydrolysate showed an antihypertensive property by inhibition of angiotensin-I-converting enzyme activity, resulting in a decrease in blood pressure. Furthermore, protein hydrolysates have been reported for their antioxidative property and for increasing calcium absorption (Akahoshi et al 2000;Chanput et al 2009). Therefore, ESRB might be utilized to develop physiologically functional food.…”

Preparation of alcalase hydrolysed rice bran protein concentrate and its inhibitory effect on soybean lipoxygenase activity

“…Oligopeptides, which are produced during hydrolysis of proteins, are also known to possess highly potent biological activities. Peptides derived from food proteins have provided numerous beneficial biological activities for human health, such as moderation of hypertension, bacteriolysis, antioxidation and enhancement of calcium adsorption in the digestive tract (Akahoshi et al, 2000). Proteases have been used for hydrolysis to improve some important functional properties of protein such as enhancing the solubilisation (Apinunjarupong et al, 2009), emulsifying capacities, emulsion stability, foam capacity, foam stability, water absorption, oil absorption (Zhang et al, 2012b) and antioxidant properties (Chanput et al, 2009).…”

Purification and characterisation of antioxidative peptides from unfractionated rice bran protein hydrolysates

“…Oligopeptides, which are produced during food processing or hydrolysis of proteins, are also known to possess highly potent biological activities. Numerous beneficial biological activities for human health, such as moderation of hypertension, bacteriolysis, antioxidation of fat and enhancement of calcium adsorption in digestive tract, have been found in peptides derived from food proteins (Akahoshi et al. , 2000).…”