Novel application of simulated moving bed chromatography to protein refolding

Integrated system for temperature-controlled fast protein liquid chromatography. II. Optimized adsorbents and ‘single column continuous operation’

“…The switch from batch to continuous operation promises several advantages, key of which is more efficient utilization of the bed [43]. Several continuous chromatography formats have been developed and applied for the separation of biomacromolecules thus far, including Continuous Annular Chromatography [44], Continuous Radial Flow Chromatography [43], Simulated Moving Bed in various guises [45][46][47][48][49], and Periodic Counter-current Chromatography [50]. The new addition described here, Continuous Travelling Cooling Zone Reactor Chromatography, employs a single column operated isocratically.…”

Oxidative protein refolding on size exclusion chromatography at high loading concentrations: Fundamental studies and mathematical modeling

“…Refolding buffer A was composed of 0.1 M Tris, 1 mM EDTA, 2 M urea, 0.2 M l-arginine, 3 mM cysteine, 0.3 mM cysteine buffered at pH 8.1 while in refolding buffer B, larginine is replaced by 0.1 M NaCl to investigate the implication of removing l-arginine in terms of mass transfer parameters and kinetics of refolding. The addition of NaCl was necessary to prevent non-specific interactions with the column [7,20].…”

“…For these reasons SEC has been widely used at lab scale for protein refolding in either batch or continuous mode using single or multiple column configurations (i.e. simulated moving bed) [1][2][3][4][5][6][7][8][9]. Existing work has also developed mathematical models for separation in SEC using various model proteins in their native forms [10,11].…”

Size-exclusion simulated moving bed chromatographic protein refolding

“…Presumably, owing to the awareness of the aforementioned limitations, more recent studies have been focused on sizeexclusion continuous chromatographic refolding. Continuous size-exclusion refolding has been demonstrated using preparative continuous annular chromatography (P-CAC) for a-lactalbumin (Schlegl et al, 2003) and lysozyme (Lanckriet and Middelberg, 2004), and simulated moving bed (SMB) chromatography for lysozyme (Park et al, 2005(Park et al, , 2006.…”