Novel Application of Carbon Nanotubes for Improving Resolution in Detecting Human Serum Proteins with Native Polyacrylamide Gel Electrophoresis

Guo, Yan-Dong; Huang, Lingyun; Baeyens, Willy R. G.; Delanghe, Joris; He, Dacheng; Ouyang, Jin

doi:10.1021/nl802935s

Cited by 15 publications

(12 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…, The level of haptoglobin (Hp) was dramaticlly decreased in the serum from hepatoma patients. Hp is a hemoglobin‐binding protein, and the concentrations of serum Hp in hepatoma patients are decreased . In addition, proteins a, b, and c indicated in the red dashed ovals (Fig.…”

Section: Resultsmentioning

confidence: 99%

A highly sensitive “turn‐on” fluorescent sensor for the detection of human serum proteins based on the size exclusion of the polyacrylamide gel

et al. 2013

Electrophoresis

Self Cite

View full text Add to dashboard Cite

A highly sensitive "turn-on" fluorescent sensor based on the size exclusion of the polyacrylamide gel was developed for the on-gels detection of human serum proteins after PAGE. The possible mechanism of this fluorescence sensor was illustrated and validated by utilizing five kinds of colloidal silver nanoparticles with different particle size distribution and six kinds of polyacrylamide gels with different pore size. It was attributed to that silver nanoparticles (<5 nm in diameter) had been selectively absorbed into the gel and formed the small silver nanoclusters, resulting in the red fluorescence. Using this new technique for the detection of human serum proteins after PAGE, a satisfactory sensitivity was achieved and some relatively low-abundance proteins (e.g. zinc-alpha-2-glycoprotein), which are the significant proteinic markers of certain diseases can be easily detected, but not with traditional methods. Furthermore, it was also successfully applied to distinguish between serums from hepatoma patient and healthy people. As a new protein detection technique, the colloidal silver nanoparticles based "turn-on" fluorescent sensor offers a rapid, economic, low background, and sensitive way for direct detection of human serum proteins, showing available potential and significance in the development of nanobiotechnology and proteome research.

show abstract

Section: Resultsmentioning

confidence: 99%

A highly sensitive “turn‐on” fluorescent sensor for the detection of human serum proteins based on the size exclusion of the polyacrylamide gel

et al. 2013

Electrophoresis

Self Cite

View full text Add to dashboard Cite

show abstract

“…For example, the carbon nanotubes (CNTs) were included into separation gels 29,30 to improve the resolutions of serum DNAs.…”

Section: Joule Heatingmentioning

confidence: 99%

Improvement of heat dissipation in agarose gel electrophoresis by metal oxide nanoparticles

et al. 2015

View full text Add to dashboard Cite

Joule heating is a primary limitation in the slab gel electrophoresis which is a gold standard method in biochemistry and biotechnology. In this paper, we introduced an innovative new class of heat transfer nanocomposite engineered by inclusion of metal oxide nanoparticles (NPs) in conventional separation medium (gel). The nanocomposite exhibits high thermal conductivity compared to gel itself. The results suggest a unique correlation between average particle size and thermal conductivity of metal oxide NPs with resolution improvement of the separation, i.e. reduction in Joule heating. Ceria, zirconia, tungsten oxide, and lanthania NPs were loaded into the agarose gel separately and used as a separation medium for gel electrophoresis. Among the NPs, ceria with the smallest size (5.2 nm) and highest thermal conductivity (17 W m -1 K -1 ) presented a better performance in reduction of Joule heating. By loading 0.3 % (m/v) ceria, zirconia, and tungsten oxide NPs into the agarose gel at 25 °C , the thermal conductivity of gel increased 79, 78, and 78 %, and resulted in 22, 18, and 14 % reduction in Joule heating (230 V), respectively. The overall separation efficiency and resolution increased for agarose/zirconia gel as compared with those of pure agarose gel. For example, the separation efficiency of 70 and 80 (bp) peaks increased 260 and 165 %, respectively. Also, the resolution increased from 1.65 for pure agarose gel to 6.32 for agarose/zirconia gel.

show abstract

“…Initial study of the native physicochemical properties and biological functions of individual proteins from serum is frequently accomplished after the separation by polyacrylamide gel electrophoresis (PAGE), which continues to be one of the most powerful separation techniques for complicated biological samples 11. However, the detection of some proteins is difficult because of their similar sizes or charge/mass ratios and especially the detection of the low‐abundance proteins is hard to be obtained due to the interference of the high‐abundance proteins such as human serum albumin and immunoglobulin G (IgG) 12. In addition, some proteins complexed with other components are also difficult to be detected.…”

Section: Introductionmentioning

confidence: 99%

Applications of multifunctional magnetic nanoparticles for the enrichment of proteins for PAGE separation

Sun¹,

Huang²,

Na³

et al. 2011

Electrophoresis

Self Cite

View full text Add to dashboard Cite

This paper describes the applications of multifunctional magnetic nanoparticles (MNPs) for the enrichment of low-abundance proteins for polyacrylamide gel electrophoresis (PAGE) separation. The hemoglobin-functionalized MNPs, named Hb-MNPs, were obtained based on electrostatic interactions and covalent binding between the hemoglobin (Hb) and the MNPs. It was demonstrated that the proteins in human serum were selectively conjugated to Hb-MNPs, which can be used for the selective enrichment of low-abundance proteins. Three and seven kinds of proteins were identified by MS after 1-D and 2-D PAGE, respectively. Comparing with native PAGE without the treatment of MNPs, some proteins were observed, such as human serum amyloid P component (SAP), vitamin D-binding protein, and serine peptidase inhibitor. Because the high concentration of SAP can be considered as a signal for the neurodegeneration of Alzheimer's disease, the present Hb-MNPs-based method was applied to investigate the serum level of SAP for the diagnosis of Alzheimer's disease, and the results are satisfying.

show abstract

Novel Application of Carbon Nanotubes for Improving Resolution in Detecting Human Serum Proteins with Native Polyacrylamide Gel Electrophoresis

Cited by 15 publications

References 39 publications

A highly sensitive “turn‐on” fluorescent sensor for the detection of human serum proteins based on the size exclusion of the polyacrylamide gel

A highly sensitive “turn‐on” fluorescent sensor for the detection of human serum proteins based on the size exclusion of the polyacrylamide gel

Improvement of heat dissipation in agarose gel electrophoresis by metal oxide nanoparticles

Applications of multifunctional magnetic nanoparticles for the enrichment of proteins for PAGE separation

Contact Info

Product

Resources

About