Novel Antimicrobial Treatment Strategy Based on Drug Delivery Systems for Acute Otitis Media

Kono, Masamitsu; Umar, Nafisa K.; Takeda, Saori; Ohtani, Makiko; Murakami, Daichi; Sakatani, Hideki; Kaneko, Fumie; Nanushaj, Denisa; Hotomi, Muneki

doi:10.3389/fphar.2021.640514

Cited by 9 publications

(6 citation statements)

References 82 publications

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“…Due to its high colonization potential, ability to ruin food, and resistance to antibiotics, disinfectants, and antiseptics, P. aeruginosa is frequently associated with biofilm formation nosocomial infections and food poisoning ( Morris, 2004 ; Hillier et al, 2006 ). Otitis media (OM) is the medical name for an infection of the tympanic membrane or middle ear, characterized by ear pain or discharge ( Danishyar and Ashurst, 2017 ; Sander, 2001 ; Kono et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

In vitro and in silico biopotentials of phytochemical compositions and antistaphylococcal and antipseudomonal activities of volatile compounds of Argania spinosa (L.) seed oil

Almuhayawi,

Alruhaili,

Gattan

et al. 2024

Front. Bioeng. Biotechnol.

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Active components in medicinal plants provide unlimited useful and traditional medicines. Antimicrobial activities are found in secondary metabolites in plant extracts such as argan oil. This experimental investigation aims to determine argan oil’s volatile compounds and examine their in vitro antimicrobial properties. In silico simulations, molecular docking, pharmacokinetics, and drug-likeness prediction revealed the processes underlying the in vitro biological possessions. Gas chromatography–mass spectrometry (GC/MS) was used to screen argan oil’s primary components. In silico molecular docking studies were used to investigate the ability of the selected bioactive constituents of argan oil to act effectively against Pseudomonas aeruginosa and Staphylococcus aureus (S. aureus) isolated from infections. The goal was to study their ability to interact with both bacteria’s essential therapeutic target protein. The 21 chemicals in argan oil were identified by GC/MS. Docking results for all compounds with S. aureus and P. aeruginosa protease proteins ranged from −5 to −9.4 kcal/mol and −5.7 to −9.7 kcal/mol, respectively, compared to reference ligands. Our docking result indicates that the 10-octadecenoic acid, methyl ester was the most significant compound with affinity scores of −9.4 and −9.7 kcal/mol for S. aureus and P. aeruginosa proteins, respectively. The minimal bactericidal concentration (MBC) and minimal inhibitory concentration (MIC) of argan oil were 0.7 ± 0.03 and 0.5 ± 0.01 for S. aureus and 0.4 ± 0.01 and 0.3 ± 0.02 for P. aeruginosa, respectively. We confirmed the antimicrobial properties of argan oil that showed significant growth inhibition for S. aureus and P. aeruginosa.

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Section: Introductionmentioning

confidence: 99%

In vitro and in silico biopotentials of phytochemical compositions and antistaphylococcal and antipseudomonal activities of volatile compounds of Argania spinosa (L.) seed oil

Almuhayawi,

Alruhaili,

Gattan

et al. 2024

Front. Bioeng. Biotechnol.

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“…To date, OM is commonly treated with antibiotic prescriptions that, however, can cause harmful effects like diarrhea, vomiting, or skin rash [ 10 ]. Additionally, the impact of antibiotics on hearing is still unclear, and when most aggressive OM forms occur, there is no evidence that the administration of antibiotics can reduce the necessity of surgery, e.g., the placement of ventilation tubes into the tympanic membrane [ 11 , 12 ]. Therefore, new therapeutic strategies are required to prevent OM and reduce the need for surgical interventions in the case of COM.…”

Section: Introductionmentioning

confidence: 99%

Emerging Multiscale Biofabrication Approaches for Bacteriotherapy

Rovelli,

Cecchini,

Zavagna

et al. 2024

Molecules

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Bacteriotherapy is emerging as a strategic and effective approach to treat infections by providing putatively harmless bacteria (i.e., probiotics) as antagonists to pathogens. Proper delivery of probiotics or their metabolites (i.e., post-biotics) can facilitate their availing of biomaterial encapsulation via innovative manufacturing technologies. This review paper aims to provide the most recent biomaterial-assisted strategies proposed to treat infections or dysbiosis using bacteriotherapy. We revised the encapsulation processes across multiscale biomaterial approaches, which could be ideal for targeting different tissues and suit diverse therapeutic opportunities. Hydrogels, and specifically polysaccharides, are the focus of this review, as they have been reported to better sustain the vitality of the live cells incorporated. Specifically, the approaches used for fabricating hydrogel-based devices with increasing dimensionality (D)—namely, 0D (i.e., particles), 1D (i.e., fibers), 2D (i.e., fiber meshes), and 3D (i.e., scaffolds)—endowed with probiotics, were detailed by describing their advantages and challenges, along with a future overlook in the field. Electrospinning, electrospray, and 3D bioprinting were investigated as new biofabrication methods for probiotic encapsulation within multidimensional matrices. Finally, examples of biomaterial-based systems for cell and possibly post-biotic release were reported.

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“…The conventional antibiotic treatment for ear infections may need to be re-evaluated after this sighting. 1 , 18 Recent antibiotic treatment of acute otitis media, children in daycare centers, wintertime infections, and AOM among children under two years of age are all risk factors for resistant bacteria. 19 , 20 In order to eradicate drug-resistant Streptococcus pneumoniae , a greater dose of amoxicillin (80 mg per kg per day) may be necessary.…”

Section: Introductionmentioning

confidence: 99%

Molecular Profile and the Effectiveness of Antimicrobials Drugs Against Staphylococcus aureus and Pseudomonas aeruginosa in the Diagnostic Approaches of Otitis Infection

Almuhayawi¹,

Gattan²,

Alruhaili³

et al. 2023

IDR

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Background Otitis externa and otitis media are two types of ear infections that affect people of all ages, although they are more common in newborns and young children. Antibiotic usage, healthcare, and advanced age all play a role in the development of this illness. Methods Fifty-eight patients with various kinds of infections of the ears were voluntary patients attending the outpatient clinics of the Prince Mutaib Bin Abdulaziz Hospital in Sakaka, Al Jouf, Saudi Arabia, examined to evaluate the role of bacteria and the likely significance of plasmids in their antibiotic resistance as ear infectious agents. Results Staphylococcus aureus and Pseudomonas aeruginosa are the most prevalent bacteria found in ear infections. The greatest number of major bacterial isolates were S. aureus (54%), followed by P. aeruginosa (13%), whereas a smaller number of isolates (3%) were from Streptococcus pyogenes, Bacillus subtilis , and Proteus vulgaris , respectively. Mixed growth was noted in 3.4% of instances. The isolation rate for Gram-positive organisms was 72%, while the rate for Gram-negative species was 28%. All the isolates had DNA greater than 14 kilobases. Hind III analysis of the plasmid DNA extracted from the resistant strains of ear infection demonstrated that antibiotic-resistance plasmids were extensively dispersed. Exotoxin A PCR amplification indicated 396 pb PCR-positive DNA for all identified samples, with the exception of three strains for which no band was observed. Patients in the epidemiological study ranged in number, but all were linked together for the purposes of the study because of their shared epidemiological characteristics. Conclusion Vancomycin, linezolid, tigecycline, rifampin, and daptomycin are all antibiotics that have been shown to be effective against S. aureus and P. aeruginosa . Microbiological pattern evaluation and antibiotic sensitivity patterns of the microorganisms providing empirical antibiotics are becoming increasingly crucial to minimize issues and the development of antibiotic-resistant strains.

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Novel Antimicrobial Treatment Strategy Based on Drug Delivery Systems for Acute Otitis Media

Cited by 9 publications

References 82 publications

In vitro and in silico biopotentials of phytochemical compositions and antistaphylococcal and antipseudomonal activities of volatile compounds of Argania spinosa (L.) seed oil

In vitro and in silico biopotentials of phytochemical compositions and antistaphylococcal and antipseudomonal activities of volatile compounds of Argania spinosa (L.) seed oil

Emerging Multiscale Biofabrication Approaches for Bacteriotherapy

Molecular Profile and the Effectiveness of Antimicrobials Drugs Against Staphylococcus aureus and Pseudomonas aeruginosa in the Diagnostic Approaches of Otitis Infection

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