Novel antimicrobial cruzioseptin peptides extracted from the splendid leaf frog, Cruziohyla calcarifer

Cuesta, Sebastián; Reinoso, Camila; Morales, Felipe; Pilaquinga, Fernanda; Morán-Marcillo, Giovanna; Proaño-Bolaños, Carolina; Blasco-Zúñiga, Ailín; Rivera, Miryan; Meneses, Lorena

doi:10.1007/s00726-021-02986-w

Cited by 18 publications

(17 citation statements)

References 56 publications

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“…Similarly, the action mechanism for cell lysis revealed that both ACAP-V and ACAP-IV interact with chain A of bacterial cell membrane extrinsic protein (PDB Id: 2dw6). Related findings were reported for cruzioseptin peptides ( Cuesta et al, 2021 ) and flocculating polypeptides ( Garcia et al, 2019 ). This bacterial cell membrane enzyme (PDB Id: 2dw6) is vital in the synthesis of unsaturated fatty acids, a key component of the phospholipid bilayer ( Kopp et al, 2020 ), through a series of dehydration and isomerization reactions.…”

Section: Discussionsupporting

confidence: 78%

ADMET profiling and molecular docking of potential antimicrobial peptides previously isolated from African catfish, Clarias gariepinus

Okella

Okello²,

Mtewa³

et al. 2022

Front. Mol. Biosci.

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Amidst rising cases of antimicrobial resistance, antimicrobial peptides (AMPs) are regarded as a promising alternative to traditional antibiotics. Even so, poor pharmacokinetic profiles of certain AMPs impede their utility necessitating, a careful assessment of potential AMPs’ absorption, distribution, metabolism, excretion, and toxicity (ADMET) properties during novel lead exploration. Accordingly, the present study utilized ADMET scores to profile seven previously isolated African catfish antimicrobial peptides (ACAPs). After profiling, the peptides were docked against approved bacterial protein targets to gain insight into their possible mode of action. Promising ACAPs were then chemically synthesized, and their antibacterial activity was validated in vitro utilizing the broth dilution method. All seven examined antimicrobial peptides passed the ADMET screening, with two (ACAP-IV and ACAP-V) exhibiting the best ADMET profile scores. The ACAP-V had a higher average binding energy (−8.47 kcal/mol) and average global energy (−70.78 kcal/mol) compared to ACAP-IV (−7.60 kcal/mol and −57.53 kcal/mol), with the potential to penetrate and disrupt bacterial cell membrane (PDB Id: 2w6d). Conversely, ACAP-IV peptide had higher antibacterial activity against E. coli and S. aureus (Minimum Inhibitory Concentration, 520.7 ± 104.3 μg/ml and 1666.7 ± 416.7 μg/ml, respectively) compared to ACAP-V. Collectively, the two antimicrobial peptides (ACAP-IV and ACAP-V) are potential novel leads for the food, cosmetic and pharmaceutical industries. Future research is recommended to optimize the expression of such peptides in biological systems for extended evaluation.

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Section: Discussionsupporting

confidence: 78%

ADMET profiling and molecular docking of potential antimicrobial peptides previously isolated from African catfish, Clarias gariepinus

Okella

Okello²,

Mtewa³

et al. 2022

Front. Mol. Biosci.

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“…In this study, SPE cartridges enhanced the recovery of such peptides. It is therefore not surprising that ACAPs all show more than 54% hydrophobicity, a value much higher than those in the previously reported peptides like Cruzioseptin (Cuesta et al, 2021), A15_B (Okella et al, 2020), OVTp12 (Ma et al, 2020) among others. Interestingly, four (ACAP-I, ACAP-II, ACAP-IV, and ACAP-VII) out of the seven identified ACAPs demonstrated α-helix structure conformation.…”

Section: Discussionmentioning

confidence: 57%

Identification of Antimicrobial Peptides Isolated From the Skin Mucus of African Catfish, Clarias gariepinus (Burchell, 1822)

et al. 2021

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Antimicrobial peptides (AMPs) constitute a broad range of bioactive compounds in diverse organisms, including fish. They are effector molecules for the innate immune response, against pathogens, tissue damage and infections. Still, AMPs from African Catfish, Clarias gariepinus, skin mucus are largely unexplored despite their possible therapeutic role in combating antimicrobial resistance. In this study, African Catfish Antimicrobial peptides (ACAPs) were identified from the skin mucus of African Catfish, C. gariepinus. Native peptides were extracted from fish mucus scrapings in 10% acetic acid (v/v) and ultra-filtered using 5 kDa molecular weight cut-off membrane. The extract was purified using C18 Solid-Phase Extraction. The antibacterial activity was determined using the Agar Well Diffusion method and broth-dilution method utilizing Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922). Thereafter, Sephadex G-25 gel filtration was further utilized in bio-guided isolation of the most active fractions prior to peptide identification using Orbitrap Fusion Lumos Tribrid Mass Spectrometry. The skin mucus extracted from African Catfish from all the three major lakes of Uganda exhibited antimicrobial activity on E. coli and S. aureus. Lake Albert’s C. gariepinus demonstrated the best activity with the lowest MIC of 2.84 and 0.71 μg/ml on S. aureus and E. coli, respectively. Sephadex G-25 peak I mass spectrometry analysis (Data are available via ProteomeXchange with identifier PXD029193) alongside in silico analysis revealed seven short peptides (11–16 amino acid residues) of high antimicrobial scores (0.561–0.905 units). In addition, these peptides had a low molecular weight (1005.57–1622.05 Da) and had percentage hydrophobicity above 54%. Up to four of these AMPs demonstrated α-helix structure conformation, rendering them amphipathic. The findings of this study indicate that novel AMPs can be sourced from the skin mucus of C. gariepinus. Such AMPs are potential alternatives to the traditional antibiotics and can be of great application to food and pharmaceutical industries; however, further studies are still needed to establish their drug-likeness and safety profiles.

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“…Over the years, some antimicrobial peptides have been identi ed in the skin of different amphibian species. For example, dermatoxin, dermaseptin, plasticin, phyloseptin and cruzioseptin are peptides extracted from some amphibian species' skin [9]. Cruzioseptin (CZS) is named for its origin in Cruziohyla from the species Cruziohyla calcarifer from northeastern Ecuador, western Panama and southeastern Costa Rica.…”

Section: Introductionmentioning

confidence: 99%

Computational Modelling of Cruzioseptin-4 Extracted From the Frog Cruziohyla Calcarifer and Pictuseptin-1 Extracted From the Frog Boana Picturatus

RENGIFO-LEMA,

PROAÑO-BOLAÑOS,

CUESTA

et al. 2023

Preprint

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A computational study of the peptides Cruzioseptin-4 and Pictuseptin-1, identified in Cruziohyla calcarifer and Boana picturatus respectively, has been carried out. The studies on cruzioseptin-4 show that it is a cationic peptide with a chain of 23 amino acids that possess 52.17% of hydrophobic amino acids and a charge at pH 7 of + 1.2. Similarly, pictuseptin-1 is a 22 amino acids peptide with a charge at pH 7 of + 3 and 45.45% of hydrophobic amino acids. Furthermore, the predominant secondary structure for both peptides is alpha-helical. Finally, the molecular docking study indicates that the viable mechanism of action for both peptides is through a targeted attack on the cell membrane of pathogens via electrostatic interactions with different membrane components, leading to cell lysis. The physicochemical properties were predicted using Pep Calc and Biosyn; secondary structures using Jpred 4 and Predict Protein; while molecular docking was performed using Autodock VINA. Geometry optimization of the peptides was done using the ONIOM hybrid method with the HF/6-31G basis set implemented in the Gaussian09 program.

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Novel antimicrobial cruzioseptin peptides extracted from the splendid leaf frog, Cruziohyla calcarifer

Cited by 18 publications

References 56 publications

ADMET profiling and molecular docking of potential antimicrobial peptides previously isolated from African catfish, Clarias gariepinus

ADMET profiling and molecular docking of potential antimicrobial peptides previously isolated from African catfish, Clarias gariepinus

Identification of Antimicrobial Peptides Isolated From the Skin Mucus of African Catfish, Clarias gariepinus (Burchell, 1822)

Computational Modelling of Cruzioseptin-4 Extracted From the Frog Cruziohyla Calcarifer and Pictuseptin-1 Extracted From the Frog Boana Picturatus

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