Novel Anoxybacillus flavithermus AK1: A Thermophile Isolated from a Hot Spring in Saudi Arabia

Khalil, Amjad; Sivakumar, Neelamegam; Arslan, Muhammad; Qarawi, Sami

doi:10.1007/s13369-017-2622-z

Cited by 7 publications

(4 citation statements)

References 26 publications

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“…Further, the growth curve of the A7 strain indicated that this isolate is able to survive at a temperature range between 37°C to 65°C (Figure 4). This growth temperature range fits the range for moderate thermophiles (45-85°C), as Khalil et al (2017) reported. Meanwhile, hyperthermophiles and extreme thermophiles grew at 85-100 °C and >100°C, respectively.…”

Section: Resultssupporting

confidence: 87%

“…Notably, some Anoxibacillus genus were also isolated from hot springs. Previously, the A. flavithermus was reported to be able to survive in temperatures ranging from 55°C to 60°C (Khalil et al 2017). Anoxybacillus sp, was also isolated from Gazlıgöl hot spring in Turkey, and they produced some hydrolytic enzymes, including amylases, specifically α-amylase (Özdemir et al 2012;Bekler et al 2021), along with xylanase and pullulanase (Chai et al 2012;Ellis and Magnuson 2012;Goh et al 2013).…”

Section: Resultsmentioning

confidence: 99%

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Screening, isolation, and characterization of amylase-producing bacteria from Poring Hot Spring Sabah, Malaysia

et al. 2022

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Abstract. Fazal BZ, Budiman C, Amin Z, Ling CMW. 2022. Screening, isolation, and characterization of amylase-producing bacteria from Poring Hot Spring Sabah, Malaysia. Biodiversitas 23: 2807-2815. Thermostable ?-amylases are being used in a wide range of industries, including food, textiles, detergents, pharmaceuticals, and fine chemicals. A good source of thermostable ?-amylases in thermophilic bacteria is found in high-temperature habitats like hot springs. Hence, this study aimed to screen, isolate, and characterize amylase-producing bacteria (APB) from the Poring hot spring in Sabah. Sediment and water samples were collected from the hot springs, serially diluted, plated onto the Luria Bertani agar medium containing starch, and incubated at 60°C for 48 hours. The amylase-producing bacterium was identified by the halo formation around the colony after the agar medium was stained with Lugol's solution. Nine colonies were found to be able to form halo zones, with a creamy colony (A7 strain) producing the highest amylolytic index (4.24). Further characterization of the A7 strain showed that the isolate was a Gram-positive, rod-shaped bacterium, with a positive reaction upon oxidase and catalase tests. The 16S rRNA sequence showed that the A7 strain had 99.81% similarity with the Anoxybacillus flavithermus, and therefore identified as A. flavithermus A7 strain. Further, the growth curve analysis indicated that the A7 strain grew well at 60°C. The 3, 5-dinitrosalicyclic acid (DNS) assay showed the crude enzyme secreted by the A7 strain exhibited optimum amylase activity at 60°C with 8.6 x 10-2 U/ml. This is the first APB obtained from hot springs in Sabah and promising for further studies and applications.

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Screening, isolation, and characterization of amylase-producing bacteria from Poring Hot Spring Sabah, Malaysia

et al. 2022

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“…Brevibacillus borstelensis AK1 (strain 11) and Anoxybacillus flavithermus AK1 (strain 40) were isolated from hot springs in Saudi Arabia. 13,14…”

Section: Bacterial Strainsmentioning

confidence: 99%

Removal of heavy metals from contaminated water by thermophilic bacteria isolated from hot springs in Saudi Arabia

Khalil¹,

Shaikh²,

Tawabini³

2022

JBMOA

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The xenobiotic and heavy metals are major source of pollutants cause severe illness to the human body and harm ecosystem. Therefore, a study was designed to investigate role of microbes in removal to these contaminants. Two thermophilic bacterial strains (Brevibacillus borstelensis AK1.) and Anoxybacillus flavithermus AK1) were isolated from the Hot Springs of Saudi Arabia and categorised as S11 and S40 respectively. The strains were tested against eight heavy metals via Minimal inhibitory concentrations (MICs) to determine heavy metal resistance. Study revealed that B. borstelensis AK1 efficiently biodegrade Chromium and Nickle with MICs of 3 mm while A. flavithermus actively involved in removal of Chromium with MIC of 3.5 mm. In distilled water, the maximum removal (90%) of Aluminum was achieved by both bacterial strains. The S40 strain was effectively remove 21.6% manganese and 50.9% cadmium. The strain S11 was capable to remove 6.1% manganese. The highest rate of removal of Lead (97.4%) and aluminum (98.5%) was observed by S11 strains. The whole study was conducted on 24-hour incubation utilizing antibiotics cinoxacin, ciprofloxacin (70 µgmL-1) and ampicillin was (3 µgmL-1) for both strains (S11 and S40) as positive control. The current study provides baseline data regarding role of thermophiles in heavy metals removal from water and in future more studies are required to identify the genetic aspects and related pathways associated with the heavy metals removal.

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“…Brevibacillus borstelensis AK1 (strain 11) and Anoxybacillus flavithermus AK1 (strain 40) were isolated from hot springs in Saudi Arabia. 13,14…”

Section: Bacterial Strainsmentioning

confidence: 99%

Untitled

2022

JBMOA

View full text Add to dashboard Cite

The xenobiotic and heavy metals are major source of pollutants cause severe illness to the human body and harm ecosystem. Therefore, a study was designed to investigate role of microbes in removal to these contaminants. Two thermophilic bacterial strains (Brevibacillus borstelensis AK1.) and Anoxybacillus flavithermus AK1) were isolated from the Hot Springs of Saudi Arabia and categorised as S11 and S40 respectively. The strains were tested against eight heavy metals via Minimal inhibitory concentrations (MICs) to determine heavy metal resistance. Study revealed that B. borstelensis AK1 efficiently biodegrade Chromium and Nickle with MICs of 3 mm while A. flavithermus actively involved in removal of Chromium with MIC of 3.5 mm. In distilled water, the maximum removal (90%) of Aluminum was achieved by both bacterial strains. The S40 strain was effectively remove 21.6% manganese and 50.9% cadmium. The strain S11 was capable to remove 6.1% manganese. The highest rate of removal of Lead (97.4%) and aluminum (98.5%) was observed by S11 strains. The whole study was conducted on 24-hour incubation utilizing antibiotics cinoxacin, ciprofloxacin (70 µgmL -1 ) and ampicillin was (3 µgmL -1 ) for both strains (S11 and S40) as positive control. The current study provides baseline data regarding role of thermophiles in heavy metals removal from water and in future more studies are required to identify the genetic aspects and related pathways associated with the heavy metals removal.

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Novel Anoxybacillus flavithermus AK1: A Thermophile Isolated from a Hot Spring in Saudi Arabia

Cited by 7 publications

References 26 publications

Screening, isolation, and characterization of amylase-producing bacteria from Poring Hot Spring Sabah, Malaysia

Screening, isolation, and characterization of amylase-producing bacteria from Poring Hot Spring Sabah, Malaysia

Removal of heavy metals from contaminated water by thermophilic bacteria isolated from hot springs in Saudi Arabia

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