Novel and sensitive high-performance liquid chromatographic method based on electrochemical coulometric array detection for simultaneous determination of catecholamines, kynurenine and indole derivatives of tryptophan

Antidepressant-Like Effect of Onion (Allium cepaL.) Powder in a Rat Behavioral Model of Depression

“…Neurotransmitter levels in the hypothalamus region were analyzed by a high-performance liquid chromatograph (HPLC) coupled with a coulometric array detector, using the method of Lakshmana and Raju 15) and Vaarmann et al 16) with some modifications. Briefly, the hypothalamus (50 mg) was mixed with 0.5 ml of ice-cold 0.12 M perchloric acid containing 0.1% cysteine, and 0.5 nmol of isoproterenol was then added as an internal standard.…”

Enhancement of chemiluminescence of the KIO4–luminol system by gallic acid, acetaldehyde and Mn2+: application for the determination of catecholamines

“…Because of the significance of catecholamines in clinical diagnosis and medical treatment, rapid and sensitive determination of these compounds in biological samples and pharmaceutical formulations is therefore very important. A variety of techniques, such as spectrophotometry, [1,2] fluorimetry [3,4] and electrochemistry, [5,6] have been utilized for the detection of catecholamines. However, some of these methods lack sensitivity and selectivity or require derivation steps or sophisticated instruments.…”

Determination of catecholamines by CE with direct chemiluminescence detection

“…Therefore, the determination of catecholamines in biological fluids is very important. Different methods, such as flow injection chemiluminescence (CL) detection [3,4], HPLC coupled with CL [5][6][7], fluorescence [8], and electrochemical (EC) [9,10] detection, have been used for the determination of catecholamines.…”