Novel amino- and thio(substituted)-1,4-naphthoquinone (NQ) compounds: Synthesis and characterization

Synthesis, Characterization and Apoptosis Capabilities of a Novel Naphthoquinone‐Derived Compound in Triple‐Negative Breast Cancer

“…[34] Loss of mitochondrial integrity is observed in cells following apoptotic stimulation. For the mitochondrial membrane potential test, MDA-MB-231 cells were seeded into 6-well cell culture dishes at a cell number of 1×10 5 . The cells were treated with IC 90 doses of ZP-2 compound and incubated for 12 and 24 h at 37 °C, 5 % CO 2 .…”

“…Therefore, the level of phosphorylated γ-H2AX is valuable in assessing DNA repair. MDA-MB-231 cells were seeded into 6-well cell culture dishes as 1×10 5 . Cells were incubated for 24 h at 37 °C, 5 % CO 2 , then the ZP-2 compound was treated with IC 90 dose and incubated for 12 and 24 h at 37 °C, 5 % CO 2 .…”

“…Annexin-V is a Ca 2 + binding protein with a high affinity for phosphatidylserine (PS), and apoptotic cells can be visualized by labeling it with a fluorescent substance. The MDA-MB-231 cells were seeded into each of 6 wells as 1×10 5 and incubated at 37 °C, 5 % CO 2 for 24 h, then treated with the ZP-2 compound at IC 90 dose and incubated at 37 °C, 5 % CO 2 for 12 and 24 h. At the end of the incubation, apoptotic levels of cells were measured with Muse Annexin V & Dead Cell Kit by the Muse™ Cell Analyzer device.…”

“…The 1,4-naphthoquinone class includes natural and synthetic derivatives with biologically active properties, including antifungal, antimalarial, antiviral, antibacterial, antimicrobial, anticancer, and antiproliferative effects. [5,6] NQ compounds, such as Adriamycin (Doxorubicin), Mitomycin C, and Daunomycin, are used as chemotherapeutic agents. [7][8][9] Our previous studies have demonstrated the synthesis of NQ compounds and their biological activities.…”

“…Naphthoquinones (NQs) are promising cancer treatment compounds which induce cell death by inhibiting topoisomerase II and increasing intracellular ROS levels. The 1,4‐naphthoquinone class includes natural and synthetic derivatives with biologically active properties, including antifungal, antimalarial, antiviral, antibacterial, antimicrobial, anticancer, and antiproliferative effects [5,6] . NQ compounds, such as Adriamycin (Doxorubicin), Mitomycin C, and Daunomycin, are used as chemotherapeutic agents [7–9] .…”