Novel amelogenin-releasing hydrogel for remineralization of enamel artificial caries

Fan, Yuwei; Wen, Zezhang T.; Liao, Shunbao; Lallier, Thomas E.; Hagan, Joseph; Twomley, Jefferson T; Zhang, Jianfeng; Sun, Zhi; Xu, Xiaoming

doi:10.1177/0883911512458050

Cited by 39 publications

(36 citation statements)

References 45 publications

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“…Several strategies have been proposed to replicate the enamel microstructure using chemicals, polymers, amorphous calcium phosphate (ACP) nanoparticles, dendrimers, and hydrogels. 7 New approaches to enamel remineralization have included the use of b-sheet forming peptides, 8 electrospun mats of ACP/poly (vinylpyrrolidone) nanofibers, 9 amelogenin-releasing system, 10 and casein phosphopeptide stabilized ACP. 11 Yamagishi et al have developed a dental paste that upon application on the enamel surface, under acidic pH, resulted in the formation of organized nanocrystals of apatite.…”

Section: Introductionmentioning

confidence: 99%

Repairing human tooth enamel with leucine-rich amelogenin peptide–chitosan hydrogel

et al. 2016

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We have recently reported the repair of carious enamel using a full-length amelogeninchitosan hydrogel through guided stabilization and growth of mineral clusters. The objective of this study was to further evaluate the enamel repair potential of smaller amelogenin peptides like LRAP (leucine-rich amelogenin peptide) and compare their efficiency with their full-length counterpart. The demineralized tooth slices treated with a single application of LRAP-chitosan hydrogel for 3 days showed a dense mineralized layer consisting of highly organized enamel-like apatite crystals. Focus-ion beam technique showed a seamless growth at the interface between the repaired layer and native enamel. There was a marked improvement in the surface hardness after treatment of the demineralized sample with almost 87% recovery of the hardness value to that of sound enamel sections. This current approach can inspire the design of smaller peptide analogues based on naturally occurring amelogenin as a competent, low-cost, and safe strategy for enamel biomimetics to curb the high prevalence of incipient dental caries.

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Section: Introductionmentioning

confidence: 99%

Repairing human tooth enamel with leucine-rich amelogenin peptide–chitosan hydrogel

et al. 2016

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“…Fan et al developed an agarose−amelogenin hydrogel model to regenerate the enamel-like tissue. 23 In their model, agarose was used as a releasing agent to investigate the function of amelogenin in the remineralization of early enamel caries. Although the agarose hydrogel containing calcium and phosphate without amelogenin was used as the control group, the different concentration of the inorganic ions and the different diffusion mode gave different results between our study and theirs.…”

Section: Discussionmentioning

confidence: 99%

Agarose Hydrogel Biomimetic Mineralization Model for the Regeneration of Enamel Prismlike Tissue

Cao

Mei

et al. 2013

ACS Appl. Mater. Interfaces

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Laboratory studies have demonstrated that enamel-like mineralized tissue can be regenerated and used to repair enamel loss. This has implications for the management of noncarious tooth loss resulting from dental erosion, attrition, and abrasion. In this study, we designed a hydrogel biomimetic mineralization model for the regeneration of enamel-like mineralized tissue with a prismatic structure. The mineralized tissue, which was generated by the model on an etched enamel surface in the presence of 500 ppm fluoride, was analyzed with scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and the nanoindentation hardness test. The generated tissue had enamel prismlike layers containing well-defined hexagonal hydroxyapatite crystals. The modulus of elasticity and the nanohardness of the regenerated enamel prismlike tissue were similar to those of natural enamel. Thus, the regeneration of enamel using this hydrogel biomimetic mineralization model is a promising approach for the management of enamel loss.

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“…CPP‐ACP serves as a supersaturation calcium and phosphate reservoir on the enamel surface and promotes the mineralization of enamel subsurface lesions . Amelogenin can stabilize the ACP and alter the aggregation to produce oriented rod‐like HAP crystals . Kim et al reported that amelogenin can promote the formation of a well‐organized enamel‐like structure when it is added to mineralization medium containing fluoride,[129b] and an amelogenin‐containing chitosan hydrogel has also been developed for enamel regeneration that can promote the assembly of enamel‐like layers on mature enamel .…”

Section: Applications Of Biomineralizationmentioning

confidence: 99%

Biomineralization: From Material Tactics to Biological Strategy

et al. 2017

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Biomineralization is an important tactic by which biological organisms produce hierarchically structured minerals with marvellous functions. Biomineralization studies typically focus on the mediation function of organic matrices on inorganic minerals, which helps scientists to design and synthesize bioinspired functional materials. However, the presence of inorganic minerals may also alter the native behaviours of organic matrices and even biological organisms. This progress report discusses the latest achievements relating to biomineralization mechanisms, the manufacturing of biomimetic materials and relevant applications in biological and biomedical fields. In particular, biomineralized vaccines and algae with improved thermostability and photosynthesis, respectively, demonstrate that biomineralization is a strategy for organism evolution via the rational design of organism-material complexes. The successful modification of biological systems using materials is based on the regulatory effect of inorganic materials on organic organisms, which is another aspect of biomineralization control. Unlike previous studies, this study integrates materials and biological science to achieve a more comprehensive view of the mechanisms and applications of biomineralization.

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Novel amelogenin-releasing hydrogel for remineralization of enamel artificial caries

Cited by 39 publications

References 45 publications

Repairing human tooth enamel with leucine-rich amelogenin peptide–chitosan hydrogel

Repairing human tooth enamel with leucine-rich amelogenin peptide–chitosan hydrogel

Agarose Hydrogel Biomimetic Mineralization Model for the Regeneration of Enamel Prismlike Tissue

Biomineralization: From Material Tactics to Biological Strategy

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