Novel Allelic Variants of Mycobacteria Isolated in Brazil as Determined by PCR-Restriction Enzyme Analysis of
            <i>hsp65</i>

Rocha, Adalgiza da Silva; Barreto, Angela Maria Werneck; Campos, Carlos Eduardo Dias; Silva, M. Villas-Bôas da; Fonseca, Leila S.; Saad, Maria Helena Féres; Degrave, Wim; Suffys, Philip Noël

doi:10.1128/jcm.40.11.4191-4196.2002

Cited by 34 publications

(16 citation statements)

References 24 publications

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“…This result is similar to that reported by da Silva Rocha et al (2002) that used the same method to characterize 20 M. kansasii clinical isolates collected from 16 different states of Brazil. The three genetic types were also the only ones detected among the 163 clinical isolates studied by Alcaide et al (1997), 111 (68%) of them were characterized as type I, 50 (31%) type II, and only 2 (1%) as type III.…”

Section: Discussionsupporting

confidence: 85%

“…During the period of the study, M. tuberculosis was the most often species identified, (82.9%) followed by MAC (9.9%) and M. kansasii (3.1%). These two species of NTM were the most frequently reported in Brazil and in another places around the world (Martínez-Moragón et al 2001, da Silva Rocha et al 2002. It is important to note that NTM frequently occur as transient commensals in the upper respiratory tract, intestine, and skin.…”

Section: Discussionmentioning

confidence: 98%

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Molecular characterization of Mycobacterium kansasii isolates in the State of São Paulo between 1995-1998

Chimara

Giampaglia

Martins

et al. 2004

Mem. Inst. Oswaldo Cruz

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 98%

Molecular characterization of Mycobacterium kansasii isolates in the State of São Paulo between 1995-1998

Chimara

Giampaglia

Martins

et al. 2004

Mem. Inst. Oswaldo Cruz

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“…Leão et al (21) reported that a variant strain of M. avium is sometimes misidentified by PRA as M. simiae II or M. kansasii I. Although allelic variations of some NTM species together with genotype sharing among many different species could be another reason for at least part of the findings in this study (7,21), it is unlikely due to the large numbers of PRA types analyzed by our group in an earlier study (7).…”

Section: Discussioncontrasting

confidence: 39%

A Comparison of Mycolic Acid Analysis for Nontuberculous Mycobacteria Identification by Thin‐Layer Chromatography and Molecular Methods

Leite

Rocha²,

Leite

et al. 2005

Microbiology and Immunology

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Nontuberculous mycobacteria (NTM), mycobacteria other than tuberculosis (MOTT), or simply atypical mycobacteria include those species not belonging to the Mycobacterium tuberculosis complex. It is widely acknowledged that as the incidence of tuberculosis decreased over the years, NTM infections have become more common (8). Even in developing countries like Brazil, where tuberculosis is still a major health care problem, NTM has emerged as the second most frequently disseminated mycobacterial infection among AIDS patients (11). Delays in an NTM diagnosis may Abstract: The development of fast, inexpensive, and reliable tests to identify nontuberculous mycobacteria (NTM) is needed. Studies have indicated that the conventional identification procedures, including biochemical assays, are imprecise. This study evaluated a proposed alternative identification method in which 83 NTM isolates, previously identified by conventional biochemical testing and in-house M. avium IS1245-PCR amplification, were submitted to the following tests: thin-layer chromatography (TLC) of mycolic acids and PCR-restriction enzyme analysis of hsp65 (PRA). High-performance liquid chromatography (HPLC) analysis of mycolic acids and Southern blot analysis for M. avium IS1245 were performed on the strains that evidenced discrepancies on either of the above tests. Sixty-eight out of 83 (82%) isolates were concordantly identified by the presence of IS1245 and PRA and by TLC mycolic acid analysis. Discrepant results were found between the phenotypic and molecular tests in 12/83 (14.4%) isolates. Most of these strains were isolated from non-sterile body sites and were most probably colonizing in the host tissue. While TLC patterns suggested the presence of polymycobacterial infection in 3/83 (3.6%) cultures, this was the case in only one HPLC-tested culture and in none of those tested by PRA. The results of this study indicated that, as a phenotypic identification procedure, TLC mycolic acid determination could be considered a relatively simple and cost-effective method for routine screening of NTM isolates in mycobacteriology laboratory practice with a potential for use in developing countries. Further positive evidence was that this method demonstrated general agreement on MAC and M. simiae identification, including in the mixed cultures that predominated in the isolates of the disseminated infections in the AIDS patients under study. In view of the fact that the same treatment regimen is recommended for infections caused by these two species, TLC mycolic acid analysis may be a useful identification tool wherever molecular methods are unaffordable.

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“…In two separate studies, Da Silva Rocha et al (8,9) Species identification. Species identification was confirmed by sequencing of region V of the rpoB gene, as described by Adékambi et al (2).…”

mentioning

confidence: 99%

Diversity of Pulsed-Field Gel Electrophoresis Patterns of Mycobacterium abscessus Type 2 Clinical Isolates

et al. 2011

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Outbreaks of infections caused by rapidly growing mycobacteria (RGM) related to surgical and cosmetic procedures have concerned Brazilian health care authorities since 2003 (5, 12, 19, 22-24, 28). More than 2,000 notifications received between 2004 and 2008 by the Brazilian National Health Surveillance Agency (ANVISA) reported cases of RGM surgical-site infections after laparoscopic, arthroscopic, or plastic surgeries (4, 19). Most isolates recovered from surgical patients studied so far showed three common features: an uncommon pattern when they were submitted to PCR-restriction enzyme analysis of the hsp65 gene (PRA-hsp65), called Mycobacterium abscessus type 2 by Devallois et al. (10) and others (19); two characteristic substitutions [C(2683)T and T(2874)C] in the sequence of region V of the rpoB gene (2, 19, 28); and two highly similar patterns with one band difference when they were typed by pulsed-field gel electrophoresis (PFGE) using DraI and AseI restriction enzymes (5,12,19,28

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Novel Allelic Variants of Mycobacteria Isolated in Brazil as Determined by PCR-Restriction Enzyme Analysis of hsp65

Abstract: Human isolates of

Cited by 34 publications

References 24 publications

Molecular characterization of Mycobacterium kansasii isolates in the State of São Paulo between 1995-1998

Molecular characterization of Mycobacterium kansasii isolates in the State of São Paulo between 1995-1998

A Comparison of Mycolic Acid Analysis for Nontuberculous Mycobacteria Identification by Thin‐Layer Chromatography and Molecular Methods

Diversity of Pulsed-Field Gel Electrophoresis Patterns of Mycobacterium abscessus Type 2 Clinical Isolates

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