Novel alginate gel microspheres produced by impinging aerosols for oral delivery of proteins

Abstract

Lysozyme and insulin were encapsulated in alginate gel microspheres using impinging aerosols method. High loadings of around 50% weight/dry microspheres weight were obtained with encapsulation efficiencies of at least 48%. Environmental scanning electron microscopy revealed smooth spherical hydrated microspheres (30-60 µm) in diameter. No lysozyme or insulin release was measured in simulated gastric fluid (HCl, pH 1.2, 37°C). Total insulin release occurred in simulated intestinal fluid (SIF; phosphate buffer saline, pH 7.4, 37°C) in 8 h following 2 h incubation in SGF and was found to retain 75% activity using the ARCHITECT® assay. Lysozyme was released completely in SIF in 10 h following 2 h incubation in SGF and was found to exhibit at least 80% bioactivity using the Micrococcus lysodeikticus assay. The absence of protein release in HCl and the retention of high levels of biological activity demonstrate the potential of alginate gel microspheres, for improving oral delivery of biopharmaceuticals.

Natural Sunflower Pollen as a Drug Delivery Vehicle

“…Among the BSA release from coated pollen grains in simulated gastric and intestinal conditions, a more retarded release is observed in gastric conditions. A similar release profile was reported in a previous study also using alginate gel microspheres and alternatively, controlled release of drugs was also reported by constructing a self‐assembly of human serum albumin and l ‐α‐dimyristoylphosphatidic acid (DMPA) to form layer‐by‐layer assembly on drug crystals . The DMPA based multilayer approach controlled the drug release based on capsule wall thickness and this provides further evidence that greater control over protein release may be obtained by modulating the permeability of the coating layer.…”

Air Extrusion System for Ionotropic Alginate Microgel Particle Formation: A Review

“…Nitrogen gas can be applied for extrusion systems that require sterile or anaerobic conditions during the encapsulation process. Typically, these applications involve bioactive materials (bioencapsulation) such as microbial bacteria, yeast, animal cells, plant cells, food bioactives or micronutrients like fish oil and polyphenols, or pharmaceuticals 16–24. The size of droplets formed using air and nitrogen gas has not been found significantly different 25.…”

In-vitro digestion of different forms of bovine lactoferrin encapsulated in alginate micro-gel particles

“…1). This continuous micro-gel forming device was previously researched to encapsulate probiotics and pharmaceutical products (Hariyadi et al, 2012;Sohail, Turner, Coombes, Bostrom, & Bhandari, 2011). The Lf-alginate mixture was introduced from a nozzle into a close upright chamber at an air pressure of 500 kPa.…”