Novel advances in nanomaterial-based electrochemical sensing of the biomarker

Özçelikay, Göksu; Bakırhan, Nurgul K.; Özkan, Síbel A.

doi:10.1016/b978-0-12-822859-3.00004-3

Cited by 4 publications

(1 citation statement)

References 76 publications

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“…Nanomaterial-based biosensors are gaining traction to retain high sensitivity, resolution, and wider detection ranges for long-term use [5,6]. Operating in the nanoscale realm affords higher surface area to volume ratios and macromolecular crowding effects for enhanced capture of target analytes and improved sensor metrics, especially for building miniaturized dynamic health-monitoring electrochemical POC diagnostics [7]. Owing to their attractive and exceptional properties, metal-organic frameworks (MOFs) are being used as transducers or recognition elements [8] for building diagnostic nano-biosensors for high sensitivity, enhanced operational stability, and long shelf life.…”

Section: Introductionmentioning

confidence: 99%

Pysanka-Inspired Electrode Modification with Aptamer Encapsulation in ZIF-8 for Urine Creatinine Electrochemical Biosensing

Ganguly,

Paul,

Prasad

2023

Chemosensors

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Drawing inspiration from the several thousand beautiful Pysanky egg art of Ukraine, we have developed a novel material, Aptamer–Gold Nanoparticles (AuNPs)@ZIF-8, that can be used for building sensitive and highly stable POC biosensors for longitudinal health mapping. Here, we demonstrate a sensitive and specific novel electrochemical biosensor, made of a novel synthesized in situ encapsulated aptamer-AuNPs@ZIF-8 composite, for monitoring levels of creatinine (0.1–1000 μg/mL). In this work, we have reported the synthetic protocol for the first-of-a-kind in situ encapsulation of aptamer and AuNPs together in a ZIF-8 matrix, and explored the characteristic properties of this novel material composite using standard analytical techniques and its application for biosensor application. The as-synthesized material, duly characterized using various physicochemical analytical methods, portrays the characteristics of the unique encapsulation strategy to develop the first-of-a-kind aptamer and AuNP encapsulation. Non-faradaic Electrochemical Impedance Spectroscopy (EIS) and Chronoamperometry were used to characterize the interfacial electrochemical properties. The biosensor performance was first validated using artificial urine in a controlled buffer medium. The stability and robustness were tested using a real human urine medium without filtration or sample treatment. Being versatile, this Ukrainian-art-inspired biosensor can potentially move the needle towards developing the next generation of sample-in-result-out robust POC diagnostics.

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Section: Introductionmentioning

confidence: 99%

Pysanka-Inspired Electrode Modification with Aptamer Encapsulation in ZIF-8 for Urine Creatinine Electrochemical Biosensing

Ganguly,

Paul,

Prasad

2023

Chemosensors

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Advanced Diagnostic Techniques for Scrub Typhus: From Conventional Methods to Nanotechnology Solutions

Sumit,

Gupta,

Kaushal

2024

ChemistrySelect

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Scrub typhus is an acute illness caused by the bacteria Orientia tsutsugamushi and is transmitted by mites. Scrub typhus propagates via infected chigger bites (larval stage). Diagnosing scrub typhus is challenging due to its ability to mimic symptoms of other febrile illnesses. Conventional methods such as Weil‐Felix, ELISA, and PCR are available for diagnosing scrub typhus. The Weil‐Felix test was formerly used in developing nations to diagnose scrub typhus, but it is not recommended since it lacks both specificity and sensitivity. IFA and ELISA are superior for detecting scrub typhus; however, they are not as effective at detecting the illness in its early stages and require convalescent sampling to confirm positive samples. However, because the Orientia tsutsugamushi serotypes differ in their genetic makeup, PCR‐based approaches have become less applicable despite their higher specificity and sensitivity. Nanomaterial‐based biosensing could help detect scrub typhus early due to its high surface area, electrical conductance, and chemical stability. This study emphasizes the need for point‐of‐care detection of this under‐diagnosed but fatal disease, emphasizing the importance of nanobiosensors in early diagnosis. The pathophysiology of scrub typhus, standard diagnostic procedures, and the critical role that nanobiosensors play in the early detection of scrub typhus are discussed.

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