Novel 61-bp Indel of RIN2 Is Associated With Fat and Hatching Weight Traits in Chickens

Lin, Wujian; Ren, Tuanhui; Li, Wangyu; Liu, Manqing; He, Danlin; Liang, Shaodong; Luo, Wen; Zhang, Xiquan

doi:10.3389/fgene.2021.672888

Cited by 5 publications

(4 citation statements)

References 37 publications

(41 reference statements)

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“…An analysis of the expression of different genotypes in different tissues and a dual-luciferase experiment of QPCTL indicate that Oct-1, a potential transcription factor in A and B alleles, may inhibit gene expression by blocking the activity of the QPCTL promoter, which leads to significant differences in the growth rate of individuals with different genotypes in F2 resource populations. In our previous studies, we also found that intron binding to inhibitory transcription factors affect gene expression [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

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QPCTL Affects the Daily Weight Gain of the F2 Population and Regulates Myogenic Cell Proliferation and Differentiation in Chickens

Ren

Lin²,

Yang³

et al. 2022

Animals

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Molecular breeding can accelerate the process of animal breeding and improve the breeding efficiency. To date, many Indel molecular markers have been identified in livestock and poultry, but how Indels affect economic traits is not well understood. For molecular breeding, it is crucial to reveal the mechanism of action of Indels and to provide more accurate information. The purpose of this study was to investigate how the 52/224-bp multiallelic Indels of the chicken QPCTL promoter area affect the daily weight gain of chickens and the potential regulatory mechanism of the QPCTL gene. The analysis was conducted by association analysis, qPCR, dual-fluorescence assay and Western blotting. The results showed that Indels in the QPCTL promoter region were significantly associated with the daily weight gain in chickens and that QPCTL expression showed a decreasing trend in embryonic breast muscle tissues. Furthermore, QPCTL expression was significantly higher in breast muscle tissues of the AC genotype than in those of the AB and BB genotypes. Based on the transcriptional activity results, the pGL3-C vector produced more luciferase activity than pGL3-A and pGL3-B. In addition, overexpression of QPCTL promoted chicken primary myoblast (CPM) proliferation and inhibited differentiation. The results of this study suggest that Indels in the promoter region of the QPCTL gene may regulate the proliferation and differentiation of CPMs by affecting the expression of QPCTL, which ultimately affects the growth rate of chickens. These Indels have important value for the molecular breeding of chickens, and QPCTL can be used as a candidate gene to regulate and improve chicken growth and development.

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Section: Discussionmentioning

confidence: 99%

“…A 61 bp Indel in chicken RIN2 changes the transcriptional activity of RIN2 by affecting the transcription factor-binding site. This gene variation may affect fat deposition in chickens by influencing RIN2 gene expression [ 16 ]. To date, few studies have examined how Indels affect animal economic traits.…”

Section: Introductionmentioning

confidence: 99%

QPCTL Affects the Daily Weight Gain of the F2 Population and Regulates Myogenic Cell Proliferation and Differentiation in Chickens

Ren

Lin²,

Yang³

et al. 2022

Animals

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“…As previously described ( Li W. et al, 2019 ; Lin et al, 2021 ; Wei et al, 2022 ), the mixed linear model in SPSS software (version 24.0) was used to analyze the association between different genotypes of indel and the different phenotypic traits of F2 resource population from Xinghua chickens crossing with WRR chickens. The analysis model was as follows,

…”

Section: Methodsmentioning

confidence: 99%

Mining of chicken muscle growth genes and the function of important candidate gene RPL3L in muscle development

Lin

Xian²,

Ren³

et al. 2022

Front. Physiol.

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The birth weight of chickens does not significantly affect the weight at slaughter, while the different growth rate after birth was one of the important reasons for the difference in slaughter weight. Also, the increase in chickens’ postnatal skeletal muscle weight is the main cause of the slaughter weight gain, but which genes are involved in this biological process is still unclear. In this study, by integrating four transcriptome datasets containing chicken muscles at different developmental times or different chicken tissues in public databases, a total of nine candidate genes that may be related to postnatal muscle development in chickens were obtained, including RPL3L, FBP2, ASB4, ASB15, CKMT2, PGAM1, YIPF7, PFKM, and LDHA. One of these candidate genes is RPL3L, whose 42 bp insertion/deletion (indel) mutation significantly correlated with multiple carcass traits in the F2 resource population from Xinghua chickens crossing with White Recessive Rock (WRR) chickens, including live weight, carcass weight, half eviscerated weight, eviscerated weight, breast meat weight, wing weight, leg muscle shear force, and breast muscle shear force. Also, there was a very significant difference between different genotypes of the RPL3L 42 bp indel mutation in these trains. Further experiments showed that RPL3L was highly expressed in chicken skeletal muscle, and its overexpression could promote the proliferation and inhibit the differentiation of chicken myoblasts by regulating ASB4 and ASB15 expression. Our findings demonstrated that the RPL3L 42 bp indel may be one of the molecular markers of chicken weight-related traits.

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“…These features make Indel more convenient and more efficient for detection [ 8 ]. To date, many studies have revealed a correlation between indel variation and the growth traits, reproductive performance, and milk traits of livestock and poultry [ 9 , 10 , 11 , 12 ]. CNV as a format of structural variants has more significant impact on genetic diversity than SNP and indel [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Two Different Copy Number Variations of the SOX5 and SOX8 Genes in Yak and Their Association with Growth Traits

Zhang

Chu²,

Bao³

et al. 2022

Animals

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Copy number variation (CNV) is a structural variant with significant impact on genetic diversity. CNV has been widely used in breeding for growth traits, meat production or quality, and coat color. SRY-like box genes (SOXs) are a class of transcription factors that play a regulatory role in cell fate specification and differentiation. SOX5 and SOX8 belong to subgroups D and E of the SOXs, respectively. Previous studies have shown that SOX5 and SOX8 are essential in the development of bones. In this study, we explored the association between the growth traits and CNVs of SOX5 and SOX8 in 326 Ashidan yaks and detected mRNA expression levels in different tissues. Our results illustrated that CNVs of SOX5 and SOX8 were significantly associated with withers height at 18 months of age and chest girth at 30 months of age (p < 0.05). The CNV combination of SOX5 and SOX8 was significantly associated with withers height at 18 months of age (p < 0.01). SOX5 expression in the lung was significantly higher than in the heart, spleen, kidney, and muscle (p < 0.05). SOX8 expression in the lung was significantly higher than in the liver and muscle (p < 0.05). Our results provide evidence that the CNVs of SOX5 and SOX8 genes could be used as new markers for the selection of yak growth traits.

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Novel 61-bp Indel of RIN2 Is Associated With Fat and Hatching Weight Traits in Chickens

Cited by 5 publications

References 37 publications

QPCTL Affects the Daily Weight Gain of the F2 Population and Regulates Myogenic Cell Proliferation and Differentiation in Chickens

QPCTL Affects the Daily Weight Gain of the F2 Population and Regulates Myogenic Cell Proliferation and Differentiation in Chickens

Mining of chicken muscle growth genes and the function of important candidate gene RPL3L in muscle development

Two Different Copy Number Variations of the SOX5 and SOX8 Genes in Yak and Their Association with Growth Traits

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