Novaprep® Vial Test is a suitable liquid-based cytology medium for high risk human papillomavirus testing by Hybrid Capture 2

Prétet, Jean‐Luc; Vidal, Chrystelle; Carval, Karine Le Bail; Ramanah, R.; Carcopino, Xavier; Cartier, Isabelle; Labouyrie, Eric; Kantélip, B.; Coumes-Marquet, S.; Riethmuller, Didier; Mougin, Christiane

doi:10.1016/j.jcv.2010.08.014

Cited by 5 publications

(6 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Reports indicate that LBC is an excellent method for reliably collecting cells on glass slides from samples with a small number of cells (6–10). LBC has also been used recently for purposes other than cytological examination, for example, in molecular biological tests and in the genotyping of the human papilloma virus in patients with uterine cervical lesions (11,12). The fixation solution used in LBC is alcohol-based, so the destruction of the DNA and RNA is limited and the structure is stable for a relatively long period.…”

Section: Introductionmentioning

confidence: 99%

Introduction and utility of liquid-based cytology on aspiration biopsy of peripheral nodular lesions of the lung

et al. 2013

View full text Add to dashboard Cite

In the present study, aspiration biopsy cytology (ABC) was used for the diagnosis of peripheral nodular lesions in the lung (PNLL), and liquid-based cytology (LBC) was carried out on the material collected to evaluate it in comparison with the conventional method (CM). The subjects comprised 130 cases that underwent computed tomography (CT)-guided ABC for PNLL. A total of 73 cases received a tumor resection, with a diagnosis based on the pathology, while 57 cases were followed up, as the tumor showed no change on the radiological examinations. Biopsy samples from these patients and lavage fluid from the aspiration needles were used for analysis. Cellular material was obtained by centrifugation of the lavage fluid, and samples were prepared by two methods, direct smearing and LBC according to the ThinPrep method. The samples were categorized into three diagnoses: i) Benign, ii) suspicion of malignancy and iii) malignant. Appropriate samples were collected in 72% of cases by LBC, but only in 36% of cases by the CM. There was no marked difference in cellular images between the two methods, with the exception of a few specific cases. LBC on its own provided sensitivity at 68%, specificity at 61% and accuracy at 65%, while a combination of LBC and biopsy markedly improved these figures to 94, 81 and 84%, respectively. The introduction of LBC is considered useful for the cytopathological diagnosis of PNLL by CT-guided ABC. LBC enables the examination of appropriate samples rich in cellular components and supports a biopsy-based diagnosis. A combination of these two methods provides even higher diagnostic accuracy, and LBC is considered an excellent method to evaluate these pathological samples.

show abstract

Section: Introductionmentioning

confidence: 99%

Introduction and utility of liquid-based cytology on aspiration biopsy of peripheral nodular lesions of the lung

et al. 2013

View full text Add to dashboard Cite

show abstract

“…According to the manufacturer, the hc2 test has CE In Vitro Diagnostics (IVD) certification for cell samples harvested in 3 media, namely STM (Qiagen), PreservCyt (Hologic) and SurePath (BD). Some studies have reported the potential use of cell samples processed with different liquid-based cytology media for hrHPV testing by hc2 [ 11 , 14 – 17 ]. We report here that the performance characteristics of the hc2 assay are appropriate for its intended use with cells stored in the new Novaprep® HQ+ medium with or without DTT.…”

Section: Discussionmentioning

confidence: 99%

“…In 2010, we conducted a clinical study to assess the suitability of the LBC Novaprep® Vial Test system (Novacyt, Vélizy-Villacoublay, France) for hrHPV DNA detection in cervical samples using the hc2 test. Very high agreement for hrHPV DNA detection was found between two consecutive cervical samples deposited in the Novaprep® Vial Test and in the reference medium STM (Qiagen) [ 11 ]. Since this study, the fixative composition of the Novaprep® system has been slightly modified and the product is now called Novaprep® HQ+ Orange.…”

Section: Introductionmentioning

confidence: 99%

Validation of Novaprep® HQ+ liquid-based cytology medium for high-risk human papillomavirus detection by hc2

Guenat¹,

Launay²,

Riethmuller³

et al. 2016

Infect Agents Cancer

Self Cite

View full text Add to dashboard Cite

BackgroundPreanalytical conditions determine the reliability and validity of bioassays. Therefore, the analytic performances of biological tests need to be determined when preanalytical steps differ from those recommended by the manufacturer. The objective of the study was to assess the analytic performance of the hc2 test for the detection of high-risk HPV DNA from cells stored in the new Novaprep® HQ+ medium.MethodsRepeatability, reproducibility, method comparison and stability (-20 °C, +4 °C, +20 °C and +40 °C up to six months) were evaluated from HPV16 and HPV18 positive cell lines diluted in the Novaprep® HQ+ medium and the reference Specimen Transport Medium (STM). A series of cervical samples with atypical squamous cells of undetermined significance (ASC-US) cytology and stored in the Novaprep® HQ+ medium was also tested.ResultsCoefficients of variation for repeatability and reproducibility were less than 8 %. Method comparison showed perfect agreement in hc2 results when the HPV-positive cells were diluted in HQ+ and reference media. Stability experiments demonstrated that the storage conditions did not alter the hc2 test results. Furthermore, clinical samples were adequately preserved for hc2 testing.ConclusionsOverall, our data show that the new Novaprep HQ+ medium is suitable for high-risk HPV testing by hc2.

show abstract

“…When one of the two cytological results (CC or NPS) was abnormal, the cytological specimen remaining in the LBC vial was screened for HPV with a polymerase chain reaction (PCR) HPV screening kit (Ref: MAD-003910M, Master Diagnostica®, Granada, Spain). A previous validation of the NovaPrep fixative medium for preservation of DNA for detection and genotyping HPV was published by Prétet [11]. …”

Section: Methodsmentioning

confidence: 99%

Preliminary Study of a New, Fully Automated System for Liquid-Based Cytology: The NovaPrep® Processor System

López-Cuervo¹,

Beltran

López

et al. 2011

Acta Cytologica

View full text Add to dashboard Cite

Objective: To evaluate a fully automated system for liquid-based cytology (LBC): the NovaPrep® Processor System (NPS), which is based on the new concept of double decantation, versus conventional cytology (CC), the gold standard for cytology. Study Design: We performed a preliminary comparative study involving 1,129 female patients who underwent sampling for a Pap test; the sample was first smeared for CC and then, using the remaining specimen on the brush, for LBC with the NPS. The performances of CC and NPS were evaluated for accuracy and compared using the gold standard of a combination of one of the two methods of pathological cytology with screening for positive human papilloma virus, quantification of cells (normal and pathological), and improvement in the quality of samples and reading time. Results: The results showed improvement in sensitivity (3.81% for CC vs. 4.52% for NPS) with a specificity superior to 90% for both, a markedly decreased number of unsatisfactory specimens, notably samples containing too many inflamed cells (7.4% for CC vs. 0.5% for NPS), and a shortening of the reading time, which was three times less using NPS. Conclusion: This preliminary study showed a gain in sensitivity, a drop in the number of unsatisfactory specimens and a reduction in reading time with NPS. The results achieved using this fully automated LBC procedure are very promising and will hopefully reduce the overall cost of cervical cancer screening in the future.

show abstract

Novaprep® Vial Test is a suitable liquid-based cytology medium for high risk human papillomavirus testing by Hybrid Capture 2

Cited by 5 publications

References 27 publications

Introduction and utility of liquid-based cytology on aspiration biopsy of peripheral nodular lesions of the lung

Introduction and utility of liquid-based cytology on aspiration biopsy of peripheral nodular lesions of the lung

Validation of Novaprep® HQ+ liquid-based cytology medium for high-risk human papillomavirus detection by hc2

Preliminary Study of a New, Fully Automated System for Liquid-Based Cytology: The NovaPrep® Processor System

Contact Info

Product

Resources

About