Notizen: Eine hochempfindliche spektrophotometrische Auswertemethode zur mikrobiologischen Bestimmung der B<sub>12</sub>-Vitamine mit Euglena gracilis var. bacillaris

Heinrich, H. C.; Lahann, H.

doi:10.1515/znb-1952-0712

Cited by 17 publications

(2 citation statements)

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“…The assay is carried out in a manner rather different from that described by Hutner et al (1956); instead of measuring the growth of the Euglena population turbidimetrically, the chlorophyll from the cells is extracted and measured colorimetrically by the procedure of Heinrich & Lahann (1952). Various chlorophyll-measuring techniques resembling this procedure have of course long been familiar to marine biologists, who use them to obtain estimates of the phytoplankton standing crop in the sea (e.g.…”

Section: Methodsmentioning

confidence: 99%

A method for the measurement of vitamin B₁₂concentration in sea water

Daisley¹

1958

J. Mar. Biol. Ass.

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The vitamin B12 concentration in sea water is so very low that only the most sensitive microbiological assay techniques are likely to be capable of measuring it, but the salt concentration is so high as to be inhibitory to most of the organisms conventionally used for the microbiological assay of the vitamin. Nevertheless, the consideration that vitamin B12 concentration may have an influence on the amounts and types of life present in sea areas has persuaded several investigators to attempt its measurement.The methods so far employed have each their own advantages and dis-advantages (Droop, 1954, 1955; Lewin, 1954; Sweeney, 1954; Cowey, 1956; Adair & Vishniac, 1958). The method to be described here offers advantages in sensitivity, permitting direct measurement of the vitamin in small quantities of oceanic waters after further dilution; samples to be measured pass through comparatively few different containers and thus there is less risk of their accumulating adventitious vitamin; simple and easily cleaned apparatus is used, and much less bench-work is involved than when methods other than dilution are used for lowering the salt concentration.

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Section: Methodsmentioning

confidence: 99%

A method for the measurement of vitamin B₁₂concentration in sea water

Daisley¹

1958

J. Mar. Biol. Ass.

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“…Any sensitive photoelectric instrument which has a linear output is suitable. Beer's law is not obeyed by suspension having an absorbance >1.0 or so; divergence is bad with microbes the size of Euglena (103). In such cases, cultures are diluted until Rayleigh scattering is negligible.…”

Section: Turbidimetricmentioning

confidence: 99%

Microbiological Assays

Hutner¹,

Cury²,

Baker³

1958

Anal. Chem.

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Deutsche Gesellschaft für Physiologische Chemie. Hamburg, den 25. bis 27. September 1952

1953

Angewandte Chemie

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Hambnrg, den 96. bls 97. September 1963 Die Tagung der Gesellschaft fand im Universitatskrankenbaus Eppendorf statt. Die gleichzeitig tagende Deutsche Qesellschajt fur Neurologic Verband einen Teil ihrer Vortrage mit dem Programm der Physiologischen Chemiker, wodurch einige spezielle, klinischdiagnostische Probleme, besonders zahlreicbe Ergebnisse der papierelektrophoretischen EiweiB-Analyse diskutiert wurden. Den Tagungsvorsitz fiihrte Prof. Dr. J . Kuhnau, der in seiner Begrii-Dungsansprache die Notwendigkeit und Niitzlichkeit der nationalen Tagungen betonttl, da sie eine vertraulichere Beeprechung der Themen erlaubten, als sie auf den groDen internationalen Kongressen moglich ist. Er begriindete damit den EntschluB der Gesellschaft, die Tagung, trots des wenige Wochen vorher stattgehabten Internationalen Biochemie-Kongresses in Pans, abruhalten, und sprach die Hoffnung aus, daB Verlauf und Ergebnisse der Tagung diesen EntschluD rechtfertigten.In der gemeinsamen Eroffnungs-Sitzung der beiden Gesellschaften wurden biochemische und medizinisehe Probleme des Nervensystems behandelt. Nervensystem nnd Zehtoiheohsel

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Notizen: Eine hochempfindliche spektrophotometrische Auswertemethode zur mikrobiologischen Bestimmung der B₁₂-Vitamine mit Euglena gracilis var. bacillaris

Cited by 17 publications

References 0 publications

A method for the measurement of vitamin B₁₂concentration in sea water

A method for the measurement of vitamin B₁₂concentration in sea water

Microbiological Assays

Deutsche Gesellschaft für Physiologische Chemie. Hamburg, den 25. bis 27. September 1952

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Notizen: Eine hochempfindliche spektrophotometrische Auswertemethode zur mikrobiologischen Bestimmung der B12-Vitamine mit Euglena gracilis var. bacillaris

Cited by 17 publications

References 0 publications

A method for the measurement of vitamin B12concentration in sea water

A method for the measurement of vitamin B12concentration in sea water

Microbiological Assays

Deutsche Gesellschaft für Physiologische Chemie. Hamburg, den 25. bis 27. September 1952

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Notizen: Eine hochempfindliche spektrophotometrische Auswertemethode zur mikrobiologischen Bestimmung der B₁₂-Vitamine mit Euglena gracilis var. bacillaris

A method for the measurement of vitamin B₁₂concentration in sea water

A method for the measurement of vitamin B₁₂concentration in sea water