Abstract:In this study, artificial antigens for Bupirimate (BP) were prepared by means of two-step procedure of glutaraldehyde method. The hapten BP was covalently attached to ovalbumin and Bovine Serum Albumin (BSA), respectively abbreviated as BP-OVA and BP-BSA. In addition some improvements were raised on the conditions for BP coupling of OVA or BSA. The conjugates were identified by UV spectrophotometry and SDS-PAGE. The results preliminarily indicated that the artificial antigen for BP synthesized successfully.
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