Notice of Retraction: Application of Inter Simple Sequence Repeat (ISSR) to Detect Genetic Diversity of Tyrophagus putrescentiae (Schrank) (Acari: Acaridae)

Abstract

Tyrophagus putrescentiae (Schrank) is a dominant species in the pest mites damaging the stored products, economic crops and edible fungi. It is widely studied due to its economic importance. In this paper, the genetic structure and genetic diversity of T. putrescentiae in three geographic populations and two hosts' populations in Jiangxi Province of China were first studied by using ISSR marker. 12 primers were selected from 40 primers for their ability to produce clear and reproducible patterns of polymorphic bands. Those primers were then used for analysing genetic diversity within and among populations of T. putrescentiae collected in Ganzhou city (GZ), Jiujiang city (JJ) and another two populations sampled in meat bran and fungi from Nanchang city (NC-M and NC-F). A total of 121 DNA bands were yielded，84 of which were polymorphic and PPB was 69.42%. The levels of genetic diversity of four population could be ordered in abundance as JJ> NC-M> GZ> NC-F and A relatively high level of intraspecific genetic diversity was revealed: He=0.1576，I = 0.2259 and PPB =36.57% at population level, while He=0.2839, I=0.4118 and PPB=69.42% at species level. This study demonstrated that the level of genetic variation of the JJ population (H=0.2175; I=0.3127; PPB=51.24%) was higher than other populations. Nei's genetic diversity analysis showed a low frequency of gene flow (Gst=0.3496; Nm=0.9302) existed. Dendrogram constructed showed the population from same location could not be clustered form a specific clade. However, geographic difference caused stronger differentiation than habitat difference. The results indicated the geographic isolation influence to genetic differentiation is stronger than the host factor influence.