NOTES: Transfer of Rhizobium japonicum Buchanan 1980 to Bradyrhizobium gen. nov., a Genus of Slow-Growing, Root Nodule Bacteria from Leguminous Plants

Jordan, D. C.

doi:10.1099/00207713-32-1-136

Cited by 538 publications

(305 citation statements)

References 9 publications

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“…Nevertheless, the position of these bacteria within the Bradyrhizobium group is unclear. Within the Bradyrhizobium group, two major clusters can be distinguished : one that includes Bradyrhizobium elkanii (Kuykendall et al, 1992) and Afipia genospecies 1 and 2 (Brenner et al, 1991) and the other including Bradyrhizobium japonicum and Bradyrhizobium liaoningense (Jordan, 1982 ;Xu et al, 1995), all other Afipia species and members of the genera Nitrobacter (Grundmann et al, 2000 ;Navarro et al, 1992 ;Orso et al, 1994 ;Sorokin et al, 1998), Agromonas (Ohta & Hattori, 1983), Blastobacter (Hirsch & Mu$ ller, 1985) and Rhodopseudomonas (Hougardy et al, 2000). Moreover, several 16S rRNA gene sequences of Afipia genospecies available in GenBank (Afipia genospecies 4-14), for which no description or strain is available, are distributed among very distant groups.…”

Section: Resultsmentioning

confidence: 99%

Description of Afipia birgiae sp. nov. and Afipia massiliensis sp. nov. and recognition of Afipia felis genospecies A.

Scola¹,

Mallet²,

Grimont³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

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Section: Resultsmentioning

confidence: 99%

Description of Afipia birgiae sp. nov. and Afipia massiliensis sp. nov. and recognition of Afipia felis genospecies A.

Scola¹,

Mallet²,

Grimont³

et al. 2002

International Journal of Systematic and Evolutionary Microbiology

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“…Until 1982, all bacteria isolated from root nodules were classified in the genus Rhizobium, and speciation was based on the formation of nodules with certain host plants, establishing the 'cross-inoculation group' concept (Fred et al, 1932;Jordan, 1982). Based on morphological and physiological patterns, the bacteria were then split into the genera Bradyrhizobium, which included relatively slow growers that produced an alkaline reaction in culture medium with mannitol as carbon source, and Rhizobium, which contained fast-growing acid producers (Jordan, 1982(Jordan, , 1984.…”

Section: Introductionmentioning

confidence: 99%

“…Based on morphological and physiological patterns, the bacteria were then split into the genera Bradyrhizobium, which included relatively slow growers that produced an alkaline reaction in culture medium with mannitol as carbon source, and Rhizobium, which contained fast-growing acid producers (Jordan, 1982(Jordan, , 1984. Initially, Bradyrhizobium japonicum was the only described species within the genus (Jordan, 1982(Jordan, , 1984, but reports of a large genetic and physiological variability among strains that nodulate soybean (Glycine max) led to the description of Bradyrhizobium elkanii a few years later (Kuykendall et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

RFLP analysis of the rRNA operon of a Brazilian collection of bradyrhizobial strains from 33 legume species

Germano

Menna

Mostasso

et al. 2006

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Genetic diversity in tropical rhizobial species is still poorly known. With the aim of increasing this knowledge, three ribosomal regions of 119 strains belonging to the official Brazilian culture collection of rhizobia and classified as Bradyrhizobium based on morphological and physiological characteristics in vitro were analysed by RFLP-PCR. The strains were isolated from 33 legume species, representing nine tribes and all three subfamilies; they all form very effective N 2 -fixing nodules and 43 of them are recommended for use in Brazilian commercial inoculants as the most effective for their hosts. For the 16S rRNA gene, type and reference strains of Bradyrhizobium japonicum fell into two major clusters, joined at a level of similarity of 50 %, which included 52 strains, 90 % of which were isolated from soybean. Two other clusters, joined at a similarity of 53 %, included reference strains of Bradyrhizobium elkanii, but not USDA 76 T ; furthermore, two other major clusters were identified and all strains were clustered at a final level of similarity of only 28 %. For the intergenic spacer (IGS) between genes coding for the 16S and 23S rRNA, strains were clustered at a final level of similarity of 27 %. Reference strains of B. japonicum fell into a major group with 51 strains, 84 % isolated from soybean, with a similarity of 59 %, while strains of B. elkanii fell into another major group, with a similarity of 55 %, clustering 44 strains, 59 % of which were isolated from hosts other than soybean. New clusters were also observed for the IGS region. The largest number of differences was detected in the analysis of the 23S rRNA gene, and 16 groups and isolated strains were joined at a very low level of similarity (16 %). In a combined analysis with the three ribosomal regions, the majority of strains isolated from soybean clustered with a similarity of 54 % with type and reference strains of B. japonicum, while most strains isolated from Brazilian indigenous legume species grouped with B. elkanii at a level of similarity of 46 %. All strains were clustered at a very low level of similarity (27 %), and at least two new clusters were clearly defined. These new clusters might be related to intraspecific differences or to novel subspecies, or even to novel species; indeed, strains from one of these clusters show higher 16S rRNA gene sequence similarity to members of the genus Burkholderia. The results obtained in this study emphasize the high level of diversity of symbiotic diazotrophic bacteria in the tropics that still remains to be determined. INTRODUCTIONThe Leguminosae (known as the Fabaceae in the USA) is one of the largest families of plants, with over 18 000 species classified into around 650 genera, representing approximately one-twelfth of all known flowering plants and occupying nearly all terrestrial biomes (Polhill & Raven, 1981). Many species within this family are capable of establishing symbioses with a group of bacteria collectively called rhizobia, of which the most important feature is the capacity...

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“…The genus Bradyrhizobium was proposed by Jordan (1982) for the slow-growing root nodule bacteria, in which colonies are generally less than or equal to 1 mm on yeast extract-mannitol agar (YMA) medium after 5-10 days incubation at 28 u C. At the time of writing, the genus contained 12 defined species: Bradyrhizobium japonicum (Jordan, 1982), B. elkanii (Kuykendall et al, 1992), B. liaoningense (Xu et al, 1995), B. yuanmingense (Yao et al, 2002), B. betae (Rivas et al, 2004), B. canariense (Vinuesa et al, 2005a), B. denitrificans (van Berkum et al, 2006), B. iriomotense (Islam et al, 2008), B. jicamae, B. pachyrhizi (Ramírez-Bahena et al, 2009), B. lablabi (Chang et al, 2011) and B. cytisi (Chahboune et al, 2011). With the exception of B. betae, all species are symbiotic nitrogenfixing bacteria associated with different legumes.…”

mentioning

confidence: 99%

Bradyrhizobium daqingense sp. nov., isolated from soybean nodules

Wang

Zhang

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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Thirteen slow-growing rhizobial strains isolated from root nodules of soybean (Glycine max L.) grown in Daqing city in China were classified in the genus Bradyrhizobium based on 16S rRNA gene sequence analysis. Multilocus sequence analysis of IGS, atpD, glnII and recA genes revealed that the isolates represented a novel clade in this genus. DNA-DNA relatedness lower than 42.5 % between the representative strain CCBAU 15774 T and the type strains of the closely related species Bradyrhizobium liaoningense USDA 3622 T , Bradyrhizobium yuanmingense CCBAU 10071 T and Bradyrhizobium betae LMG 21987 T , further confirmed that this group represented a novel species. CCBAU 15774 T shared seven cellular fatty acids with the three above-mentioned species, but the fatty acids 15 : 0 iso and summed feature 5 (18 : 2v6,9c and/or 18 : 0 anteiso) were unique for this strain. The respiratory quinone in CCBAU 15774 T was ubiquinone-10 and the cellular polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, cardiolipin and unknown aminolipid, polar lipid and phospholipid. In addition, some phenotypic features could be used to differentiate the novel group from the related species. On basis of these results, we propose the name Bradyrhizobium daqingense sp. nov., with CCBAU 15774 T (5LMG 26137 T 5HAMBI 3184 T 5CGMCC 1.10947 T ) as the type strain. The DNA G+C content of the type strain is 61.2 mol% (T m ).

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NOTES: Transfer of Rhizobium japonicum Buchanan 1980 to Bradyrhizobium gen. nov., a Genus of Slow-Growing, Root Nodule Bacteria from Leguminous Plants

Cited by 538 publications

References 9 publications

Description of Afipia birgiae sp. nov. and Afipia massiliensis sp. nov. and recognition of Afipia felis genospecies A.

Description of Afipia birgiae sp. nov. and Afipia massiliensis sp. nov. and recognition of Afipia felis genospecies A.

RFLP analysis of the rRNA operon of a Brazilian collection of bradyrhizobial strains from 33 legume species

Bradyrhizobium daqingense sp. nov., isolated from soybean nodules

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