Notes- Pyridylethylated d-α-Methylphenethylamines

Shapiro, Seymour L.; Rose, Ira M.; Testa, Frank C.; Freeman, L.P.

doi:10.1021/jo01063a626

Cited by 3 publications

(4 citation statements)

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“…It would seem that more than 229; of the albumin mRNA molecules in frog liver are not polyadenylated or possess very short polyadenylate sequence. Similar findings have been reported for other mRNA species, such as ovalbumin [21], globin [22], rat albumin [23,24], and frog vitellogenin [25].…”

Section: Isolutio~i Und Purificulion Qf a Lbumin Mcswnger Rnasupporting

confidence: 88%

“…It is possible that either these albumin mRNA molecules had not been polyadenylated during the processing of the initial mRNA transcripts [38] or they reflect aging of the mRNA molecules [39]. Similar findings have been reported for other mRNA species such as ovalbumin [21,40,41], globin [22,42], casein [43], vitellogenin [25] and rat liver albumin [23,24,44] mRNAs.…”

Section: Discussionsupporting

confidence: 60%

“…The genes coding for the plasma proteins albumin and vitellogenin constitute a very interesting system in which to study the molecular events governing the hormonal regulation of gene expression [29]. As vitellogenin mRNA has already been isolated and fully characterized [25] the next step in the analysis of such a system was to isolate, purify and characterize the mRNA coding for albumin. Various methods have been described forthe isolation ofalbumin mRNAfrom rat liver [23,24,30,44] and mouse liver [31].…”

Section: Discussionmentioning

confidence: 99%

“…Frog albumin mRNA appears to be very close in size to the minimal size required by translational and structural considerations common to characterized mRNAs. Extensive noncoding sequences are not apparent, in contrast to other messenger RNAs as for ovalbumin [21,32,33], cc-crystallin [34], rat a-fetoprotein [35], rat albumin [24] and vitellogenin [25]. On the other hand Brown and Papaconstantinou [31] have also failed to find extensive noncoding sequences in the albumin mRNA of the mouse.…”

Section: Discussionmentioning

confidence: 99%

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Isolation and Characterization of Xenopus laevis Albumin mRNA

Dimitriadis

1981

European Journal of Biochemistry

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The messenger RNA for albumin was isolated from the liver of male frogs. Purification was achieved using oligo(dT)-cellulose chromatography and sucrose gradient centrifugation under denaturing conditions. As judged by translational activity in a cell-free protein-synthesizing system derived from rabbit reticulocytes, albumin mRNA was enriched 259-fold as compared to the total ribonucleic acid of the liver cells. Purified albumin mRNA migrated after sucrose gradient centrifugation as a single symmetrical peak of approximately 17 S and also moved as a single band following denaturing agarose gel electrophoresis. Albumin mRNA possesses properties compatible with the presence of a poly(adeny1ic acid) sequence. Translation in vitro yielded a product which is immunoprecipitable with anti-(frog albumin) and which showed a single radioactive peak having a molecular weight of about 74000 in sodium dodecylsulfate/polyacrylamide gel electrophoresis. Complementary DNA was synthesized using reverse transcriptase and, as a template, purified albumin mRNA. Following hybridization under conditions of excess RNA, the rotljz of albumin mRNA was found to be 1.8 x 10-3mol . s . I-'.This result also confirmed that albumin mRNA had been isolated in a highly purified form.Albumin, a major serum protein synthesized and secreted by the liver, is one of several serum proteins whose synthesis has been suggested to be regulated by hormonal [l-41, developmental [5] and nutr'itional factors [6,7]. A very interesting finding is that the synthesis of albumin in vivo decreases following estradiol treatment, particularly after secondary hormonal exposure, in contrast to vitellogenin, whose synthesis is induced by the hormone [4,8]. However, although there is some evidence that a change in the transcriptional activity of the albumin gene may be of importance in the estrogen response [7], it is not sufficient to exclude the possibility that the deinduction of albumin synthesis reflects a translational control mechanism. The simultaneous deinduction of albumin synthesis in the same cells in which vitellogenin is induced by estrogen, offers a 'built-in' control for studying differential gene transcription and translation. In order to define possible regulation mechanisms of albumin biosynthesis further, isolation of albumin mRNA and preparation of a specific complementary DNA probe against this mRNA are required. Such molecular hybridization probes, constructed by reverse transcription of messenger RNAs of specific proteins, have been used to localise specific genes in chromatin fractions and study the regulation of these genes. In this way it has been shown that modifications of chromatin structure are involved in the regulation of the transcription of the globin [9], ovalbumin [lo], and vitellogenin [Ill.In this report we describe the purification of frog liver albumin mRNA with the help of oligo(dT)-cellulose chroAbbreviations. cDNA, complementary DNA; poly(A)-rich mRNA, inRNA possessing poly(adeny1ic acid) residues; rot, product of RNA concent...

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Section: Isolutio~i Und Purificulion Qf a Lbumin Mcswnger Rnasupporting

confidence: 88%